Optimasi Kondisi Lingkungan Media Produksi Lipase Oleh Candida W 3.8 Dari Kawasan Mangrove Wonorejo Surabaya

Umar, Rurin Luswidya Artaty (2016) Optimasi Kondisi Lingkungan Media Produksi Lipase Oleh Candida W 3.8 Dari Kawasan Mangrove Wonorejo Surabaya. Undergraduate thesis, Institut Teknologi Sepuluh Nopember Surabaya.

[img]
Preview
Text
1512100037-undergraduate-theses-.pdf - Published Version

Download (1MB) | Preview

Abstract

Lipase (EC 3.1.1.3) adalah enzim kelas hidrolase, yang bekerja dalam hidrolisis triasilgliserol menjadi asam lemak dan gliserol. Lipase dapat diproduksi dari mikroorganisme. Salah satu kelompok mikroorganisme yang mampu memproduksi lipase adalah marine yeast. Produksi lipase dari marine yeast dapat dilihat dengan uji aktivitas lipase. Produksi lipase dari marine yeast dipengaruhi oleh beberapa faktor kondisi lingkungan, antara lain masa inkubasi, suhu, dan pH. Penelitian ini bertujuan mencari variasi kondisi lingkungan optimal untuk produksi lipase dari Candida W 3.8 Penelitian ini menggunakan Candida W 3.8 yang telah diremajakan, kemudian diinkubasi selama 48 jam pada suhu ruang. Isolat diinokulasi ke dalam media Yeast Malt Broth (YMB) untuk pembuatan kurva pertumbuhan. Kultur dimasukkan ke dalam media produksi dengan variasi kondisi lingkungan yang terdiri dari masa inkubasi serta pH dan suhu. Selanjutnya diuji aktivitas lipase dengan menggunakan p-Nitrophenyl Palmitate (pNPP). Absorbansi diukur dengan spektrofotometer pada panjang gelombang 410 nm. Hasil absorbansi dimasukkan ke dalam persamaan kurva standar p-Nitrophenol (pNP), selanjutnya dimasukkan ke dalam rumus aktivitas lipase. Nilai yang diperoleh dinyatakan dalam satuan aktivitas enzim yaitu U/ml. Desain penelitian menggunakan kombinasi dua faktor. Data yang diperoleh dianalisis secara deskriptif kuantitatif v Hasil penelitian didapatkan kondisi lingkungan optimal untuk produksi lipase oleh Candida W 3.8 adalah pada masa inkubasi 120 jam, serta rentang suhu 27⁰C - 45⁰C dan pH 7 =========================================================== Lipase (EC 3.1.1.3) is a hidrolase class enzyme, which works in triglyceride hydrolisis into fatty acid and glycerol. Lipase can be produced from microorganism, one of which is marine yeast. Lipase production from marine yeast can be seen in lipase activity test. It is affected by several environmental factors, such as pH, temperature, and incubation time. This research intends to look for the optimization variation on those environmental factors combination to produce lipase from Candida W 3.8 This research is conducted using rejuvenated Candida W 3.8 which was incubated for 48 hours in room temperature. Then the isolate were inoculated in media Yeast Malt Broth (YMB) in order to create growth curve. The culture then were being put into media production with various change in incubation time, pH, and temperature. Lipase activity were tested by using p- Nitrophenyl Palmitate (pNPP). Absorbency was measured by spektrofotometer on wavelength 410 nm. Absorbency result were put into equation of standard curve p-Nitrophenol (pNP), and then being used as an input on lipase activity formula. The result are stated in enzym activity unit, which is U/ml. Research design is being done by the combination of two factors, and the obtained data analyzed by using descriptive quantitative method. The optimum environment in order to produce lipase enzyme by using Candida W 3.8 is on 120 hours incubation time, temperature range in 27⁰C - 45⁰C and pH 7

Item Type: Thesis (Undergraduate)
Additional Information: RSB 572.76 Uma o-1
Uncontrolled Keywords: Aktivitas enzim, Candida W 3.8, lipase, marine yeast
Subjects: Q Science > QH Biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: EKO BUDI RAHARJO
Date Deposited: 11 Dec 2019 09:22
Last Modified: 11 Dec 2019 09:22
URI: https://repository.its.ac.id/id/eprint/72332

Actions (login required)

View Item View Item