Seleksi In Vitro Tanaman Jagung (Zea Mays L.) Varietas Talango Dan Manding Terhadap Cekaman Salinitas

Finariyah, Fathin (2015) Seleksi In Vitro Tanaman Jagung (Zea Mays L.) Varietas Talango Dan Manding Terhadap Cekaman Salinitas. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Di Indonesia, kebutuhan akan Zea mays tiap tahunnya meningkat. Hal ini berbanding terbalik dengan sedikitnya lahan pertanian yang digunakan. Solusinya yakni memanfaatkan lahan marginal dengan kandungan garam tinggi. Tujuannya adalah untuk menguji pengaruh tingkat ketahanan varietas Talango dan Manding terhadap salinitas serta melihat adanya keragaman genetik galur hasil seleksi in vitro. Pada penelitian ini, untuk mendapatkan varietas Z. mays yang toleran terhadap salinitas dilakukan dengan menggunakan teknik in vitro. Pertama dilakukan induksi kalus (MS0+4ppm 2,4-D), kemudian kalus disubkultur pada medium seleksi (MS0+4ppm 2,4-D+konsentrasi NaCl (0, 2500, 5000, dan 7500 ppm)). Selanjutnya dilakukan analisis keragaman genetik dengan RAPD. Parameter ketahanan kalus pada perlakuan dapat dilihat dari perubahan morfologi warna kalus, persentase kalus hidup, pertambahan berat kalus yang semakin menurun. Analisis RAPD menunjukkan hasil bahwa terjadi polimorfisme pada kedua varietas dengan primer OPA 13, OPB 07, OPC 02, OPK 20, dan OPU 19. Hal ini membuktikan adanya perbedaan genetik antara kalus kontrol dan toleran. ================================================================================================ In Indonesia, needs for Zea mays annually increased. It is inversely proportional to fewer agricultural land that can be used. The solution that is utilizing marginal land with high salt content. The aim of this study are to test the effect of resistance level Talango and Manding varieties to salinity and to know the genetic diversity of in vitro selection yield strains. In this study, to obtain salinity tolerant Z. mays varieties, used in vitro techniques. First, callus was induced (MS0+4ppm 2,4-D), then subcultured on selection medium (MS0+4ppm 2,4- D+NaCl concentration (0, 2500, 5000, and 7500 ppm)). Then analysis of genetic diversity used RAPD. Callus resistance parameter on treatment could be seen by the callus morphological change of color, alive callus percentage, callus weight gain decreasing with increasing salinity concentration. Analysis RAPD showed that polymorphism occurs in both varieties with primer OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. This proves the existence of genetic differences between control and tolerant callus.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 584.92 Fin s
Uncontrolled Keywords: Cekaman Salinitas, RAPD, Seleksi In vitro, Z. mays
Subjects: Q Science > QK Botany
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Yeni Anita Gonti
Date Deposited: 25 Feb 2020 07:25
Last Modified: 25 Feb 2020 07:25
URI: https://repository.its.ac.id/id/eprint/75161

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