Evaluasi Daya Hidup Dan Daya Kerja Kapang Lipolitik Aspergillus Niger Pada Medium Cair

Andhita, Revina Glady Putri (2016) Evaluasi Daya Hidup Dan Daya Kerja Kapang Lipolitik Aspergillus Niger Pada Medium Cair. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Enzim lipolitik merupakan enzim yang memiliki peranan penting dalam bioteknologi modern. Enzim ini mampu menghidrolisis ikatan ester dan memecah trigliserida menjadi asam lemak bebas dan gliserol. Enzim lipolitik banyak dihasilkan dari mikroorganisme, salah satunya adalah kapang Aspergillus niger. Tujuan dari penelitian ini adalah untuk mengetahui daya hidup dan daya kerja kapang Lipolitik Aspergillus niger pada medium minyak goreng bekas dan air pencucian ikan pada periode tertentu. Metode yang digunakan dalam penelitian ini meliputi uji viabilitas, pengukuran biomassa, pengukuran aktivitas enzim, serta pengukuran jumlah degradasi lipid. Masa inkubasi yang dilakukan pada penelitian ini selama dua bulan dengan perlakuan setiap dua minggu sekali mulai dari minggu ke-1 hingga minggu ke-9 dengan menggunakan limbah pencucian ikan dan minyak goreng bekas. Hasil penelitian menunjukkan bahwa Aspergilllus niger mampu mempertahankan viabilitasnya pada kedua medium selama 9 minggu. Aktivitas lipolitik dan degradasi lipid tertinggi yaitu sebesar 0,12U/mL dan 91%pada medium limbah pencucian ikan. ======================================================================================================== Lipolytic enzyme is an enzyme that has an important role in biotechnology. Lipolytic enzyme can hydrolyze the ester bonds and break down triglycerides into free fatty acids and glycerol. Many lipolytic enzymes produced by microorganisms, one of which is Aspergillus niger. The purpose of this study was to determine the viability and ability of lipolytic fungus Aspergillus niger in used-cooking oil and fish-washing waste water medium in certain periods. The method used in this study include the viability test, measurement of biomass, enzyme activity measurement, and measurement of lipid degradation amount. The incubation period on this study is two months. The treatment was done in every two weeks, from week 1 to week 9, using fish-washing waste and used-cooking oil. The results of this study is Aspergilllus niger able to maintain viability in both medium for 9 weeks. The highest lipolytic activity and lipid degradation is 0,12U/mL and 91% on fish-washing waste water medium.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 572.57 Rgl e 3100016066560
Uncontrolled Keywords: Aspergillus niger, enzim, kapang, lipolitik, enzyme, mold , lipolytic
Subjects: Q Science > QK Botany > QK898.L56 Lipids
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: - Davi Wah
Date Deposited: 03 Mar 2020 04:32
Last Modified: 03 Mar 2020 04:32
URI: https://repository.its.ac.id/id/eprint/75186

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