Monitoring Aktivitas Antioksidan dan Antidiabetes Fraksi dari Ekstrak Etil Asetat pada Kulit Batang Garcinia latissima Miq.

Thoyyibah, Qurrota A`yun (2020) Monitoring Aktivitas Antioksidan dan Antidiabetes Fraksi dari Ekstrak Etil Asetat pada Kulit Batang Garcinia latissima Miq. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Genus Garcinia (Fam. Cluciaciae) dikenal sebagai sumber senyawa fenolat yang telah dilaporkan memiliki berbagai aktivitas biologis yaitu antioksidan, antidiabetes, antikanker, dll. Pada penelitian ini dilakukan monitoring aktivitas antioksidan dan antidiabetes terhadap sejumlah fraksi dari ekstrak etil asetat kulit batang G. latissima Miq. (tanaman endemik pulau Maluku, Indonesia). Proses ekstraksi dilakukan dengan metode maserasi dengan pelarut etil asetat menghasilkan 240 g dari 2 kg kulit batang kering G. latissima Miq. Selanjutnya, ekstrak etil asetat difraksinasi dengan metode kromatografi cair vakum (KCV) pada kolom silika gel dan dielusi dengan campuran pelarut n-heksana:etil asetat dengan peningkatan polaritas sehingga diperoleh sembilan fraksi (A-I). Ekstrak etil asetat dan semua fraksi diuji aktivitas antioksidan dengan radikal DPPH dan antidiabetes dengan α-glukosidase dari baker’s yeast dan rat intestinal acetone powder. Data hasil monitoring menunjukkan bahwa fraksi H dan I yang merupakan fraksi polar memiliki aktivitas antioksidan dan antidiabetes terbaik daripada fraksi lainnya. Nilai penghambatan (IC50) antioksidan fraksi H 0,03 µg/mL dan fraksi I 0,06 µg/mL. Nilai penghambatan (IC50) antidiabetes dengan yeast fraksi H 102,33 µg/mL dan fraksi I 29,51 µg/mL, sedangkan IC50 antidiabetes dengan rat intestinal fraksi H 43,65 µg/mL dan fraksi I 75,86 µg/mL. ====================================================================================== The genus Garcinia (Fam. Cluciaciae) is known as a source of phenolic compounds which are reported to have biological activities: antioxidants, antidiabetic, and anticancer etc. In this study, the monitoring was carried out for the antioxidant and antidiabetic activity of a number of fractions from the ethyl acetate extract of stem bark G. latissima Miq. (endemic plants, the Maluku Islands, Indonesia). The extraction process uses the maceration method with ethyl acetate solvent produced 240 g extract from 2 kg from dry bark of G. latissima Miq. Furthermore, the ethyl acetate extract was carried out by fractionation by vacuum liquid chromatography (VLC) over the silica gel column and eluted using a mixture of n-hexane and ethyl acetate, with increasing polarity, and were obtained nine fractions (A-I). The ethyl acetate extract and the all fractions were tested for antioxidant activity using radical DPPH and antidiabetic activity using α-glukosidase from baker’s yeast and rat intestinal acetone powder. Based on monitoring data, it shown that the polar fraction H and I has the best inhibition activity than the other positive fractions. Antioxidant inhibititon value (IC50) of fraction H was 0.03 µg/mL and fraction I was 0.06 µg/mL. Antidiabetic inhibititon value (IC50) using yeast of fraction H was 102.33 µg/mL and fraction I was 29.51 µg/mL, on the other hand antidiabetic inhibititon value (IC50) using rat intestinal of fraction H was 43.65 µg/mL and fraction I was 75.86 µg/mL.

Item Type: Thesis (Masters)
Uncontrolled Keywords: antioksidan, antidiabetes, ekstrak etil asetat, Garcinia latissima, kulit batang, antioxidant, antidiabetic, ethyl acetate extract, Garcinia latissima, stem bark
Subjects: Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Q Science > QD Chemistry > QD63 Extraction
Divisions: Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis
Depositing User: Qurrota A`yun Thoyyibah
Date Deposited: 03 Sep 2020 04:21
Last Modified: 03 Sep 2020 04:21
URI: https://repository.its.ac.id/id/eprint/81726

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