Modifikasi Struktur dan Uji Antidiabetes α-mangostin Hasil Isolasi Dari Ekstrak Etil Asetat Kulit Buah Garcinia mangostana Linn.

Umami, Khoiriyah (2020) Modifikasi Struktur dan Uji Antidiabetes α-mangostin Hasil Isolasi Dari Ekstrak Etil Asetat Kulit Buah Garcinia mangostana Linn. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Garcinia mangostana Linn. (Manggis) famili Clusiaceae merupakan sumber utama senyawa santon, banyak ditemukan sebagai suplemen untuk meningkatkan kesehatan dan kebugaran tubuh, termasuk juga sebagai obat untuk penderita diabetes mellitus. Pada penelitian ini, telah dapat diisolasi dan dimurnikan dua senyawa turunan santon, melalui pemisahan dengan cara maserasi pelarut etil asetat dilanjutkan fraksinasi dan refraksinasi dengan metoda kromatografi gradien pelarut yang ditingkatkan kepolarannya. Kedua senyawa yang dihasilkan, yaitu α-mangostin (1) sebanyak (21,66 g) dan 8-deoksigartanin (2) sebanyak (0,34 g) strukturnya ditentukan menggunakan teknik spektroskopi (UV-Vis, IR, HRESIMS, 1H-NMR, 13C-NMR), dan uji aktivitas antidiabetes dilakukan dengan metode penghambatan enzim α-glukosidase, dihasilkan nilai IC50 67,56 µM (1) dan 77,95 µM (2). Hasil struktur modifikasi (1) dengan asam asetat anhidrida, diperoleh 3,6-dimetil ester α-mangostin (3) sebanyak (13,5 mg). Penentuan struktur (3) dipantau melalui spektrum 13C-NMR. Pengujian aktivitas antidiabetes dengan enzim α-glukosidase memberikan nilai IC50 32,55 µM, data ini menunjukkan bahwa aktivitas antidiabetes senyawa (3) lebih aktif dibandingkan (1) ==================================================================================================================== Garcinia mangostana Linn. (Mangosteen) Clusiaceae family is the main source of xanthones compounds, commonly found as supplements to improve health and fitness, including drug for people with diabetic mellitus. In this research, two xanthone derivatives have been isolated and purified, through separation with maceration with ethyl acetate solvent and followed by fractionation and refractionation by the solvent gradient chromatography method which is increasing polarity. The two compounds produced, namely α-mangostin (1) in the amount of (21.66 g) and 8-deoxigartanin (2) in the amount of (0.34 g) the structure is determined using spectroscopic techniques (UV-Vis, IR, HRESIMS, 1H-NMR, 13C-NMR), and the antidiabetic activity test was performed with the α-glucosidase enzyme inhibition method, resulting in IC50 values of 67.56 µM (1) and 77.95 µM (2). Structural modifications produced (1) with acetic acid anhydride obtained 3,6-dimethyl ester-α-mangostin (3) as much (13.5 mg). The structure determination (3) is monitored with the 13C-NMR spectrum. Testing antidiabetic activity with the α-glucosidase enzyme gives an IC50 value of 32.55 µM, this data shows that the antidiabetic activity of compound (3) is more active than (1)

Item Type: Thesis (Masters)
Uncontrolled Keywords: Garcinia mangostana Linn., α-mangostin, 8-deoxigartanin, 3,6-dimethyl ester-α-mangostin, antidiabetic, Garcinia mangostana Linn., α-mangostin, 8-deoksigartanin, 3,6-dimetil ester-α-mangostin, antidiabetes
Subjects: Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions: Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User: Khoiriyah Umami
Date Deposited: 30 Nov 2020 22:54
Last Modified: 01 Dec 2020 02:04
URI: https://repository.its.ac.id/id/eprint/82290

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