Kajian Literatur Imobilisasi Urease menggunakan Variasi Jenis Matriks Biokomposit

Lolita, Gracia Pradnya (2021) Kajian Literatur Imobilisasi Urease menggunakan Variasi Jenis Matriks Biokomposit. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

Text 01311740000040-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2023. Download (2MB) | Request a copy

Abstract

Urease (urea amidohydrolase, EC 3.5.1.5) merupakan nikel metaloenzim yang menghidrolisis urea menjadi amonium dan karbonat. Urease bebas bersifat tidak stabil, sehingga aplikasi dan penggunaannya dalam industri menjadi terbatas. Hal ini dapat diatasi dengan teknik imobilisasi enzim. Studi literatur ini bertujuan untuk mengumpulkan berbagai informasi dan menyajikan gambaran mengenai performa aktivitas enzimatik urease terimobilisasi dengan metode entrapment dalam berbagai jenis matriks biokomposit guar gum-alginat, guar gum-agarosa, dan guar gum-kitosan sebagai matriks karier imobilisasi. Teknik entrapment untuk imobilisasi enzim mampu mengikat enzim yang dilekatkan pada pori gel dengan ukuran yang sesuai sehingga hanya substrat dan produk dengan ukuran lebih kecil dari pori gel yang dapat berdifusi keluar masuk pori. Biopolimer komposit memiliki keunggulan dalam imobilisasi enzim yaitu dapat menghasilkan film yang stabil dan kuat serta mampu mempertahankan aktivitas enzim. Penambahan guar gum sebagai matriks biokomposit imobilisasi bersama dengan alginat, agarosa, dan kitosan berpotensi meningkatkan efisiensi imobilisasi urease.
============================================================================================================
Urease (urea amidohydrolase, EC 3.5.1.5) is a nickel metalloenzyme that hydrolyzes urea into ammonium and carbonate. Free urease tends to be unstable, hence its application and usability in the industry are limited. This issue could be solved by enzyme immobilization techniques. This literature study aims to collect information and present an overview of the enzymatic activity performance of immobilized urease by entrapment method in various types of biocomposite matrix i.e. guar gum-alginate, guar gum-agarose, and guar gum-chitosan as carrier matrizx for urease immobilization. The entrapment technique for enzyme immobilization binds the enzymes attached to the gel pores so that only substrates and products with a size smaller than the gel pores can diffuse in and out of the pores. Composite biopolymer has advantages in enzyme immobilization, as it can produce stable and strong films and can maintain enzyme activity. The addition of guar gum as an immobilized biocomposite matrix combined with alginate, agarose, and chitosan has the potential to increase the efficiency of urease immobilization.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Biokomposit, Entrapment, Imobilisasi, Matriks,Urease Biocomposite, Entrapment, Immobilization, Matrix, Urease
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Gracia Pradnya Lolita
Date Deposited:	24 Jul 2021 06:37
Last Modified:	24 Jul 2021 06:41
URI:	http://repository.its.ac.id/id/eprint/84446

Actions (login required)

View Item