Pengaruh Penambahan Endospora dan Sel Vegetatif Bakteri Karbonatogenik Untuk Memperkuat Biobeton

Aditama, Muhamad (2021) Pengaruh Penambahan Endospora dan Sel Vegetatif Bakteri Karbonatogenik Untuk Memperkuat Biobeton. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Bakteri karbonatogenik merupakan bakteri yang dapat mempresipitasi CaCO3. Beberapa spesies dapat membentuk endospora yang tahan terhadap lingkungan ekstrim, dapat menghasilkan CaCO3 yang merupakan salah satu komponen beton. Tujuan penelitian adalah mengetahui pengaruh endospora dan sel vegetatif bakteri karbonatogenik untuk memperkuat biobeton. Isolat yang digunakan adalah Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, dan Sporosarcina JA4. Produksi endospora dilakukan di media Yeast Urea (YU) broth dengan suhu 70°C selama 20 menit. Komposisi beton mengikuti SNI 7656:2012 dan uji kuat tekan beton menggunakan mesin compression testing sesuai SNI 1974-2011. Biobeton dengan penambahan endospora menghasilkan kuat tekan dan beban maksimal lebih baik daripada sel vegetative. Kuat tekan dan beban maksimal yang tertinggi terdapat pada biobeton dengan endospora Bacillus AK4 yaitu 20,93 Mpa dan 16.433,33 Kgf . ======================================================================================================== Carbonatogenic bacteria are bacteria that can precipitate CaCO3. Some species form endospores that are resistant to extreme environments and also can produce CaCO3 which is one of the components of the concrete. The purpose of this research is to find out the effect of adding carbonatogenic bacterial endospores and vegetative cell to strengthen the concrete structure. The isolates used were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosarcina JA4. Endospore production was carried out on Yeast Urea (YU) broth media at a temperature of 70°C for 20 minutes. Composition of concrete follows SNI 7656: 2012 and the compressive strength test of concrete uses a compression testing machine according to SNI 1974-2011. Bioconcrete with endospores produces better compressive strength and maximum load than vegetative cells. The highest compressive strength and maximum load were bioconcrete with Bacillus AK4 endospores, which is 20.93 Mpa and 16,433.33 Kgf.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Bakteri karbonatogenik, Biobeton, Endospora, YU broth Bioconcrete, Carbonatogenic bacteria, Endospore, YU- broth
Subjects: Q Science > QH Biology
Q Science > QR Microbiology
Q Science > QR Microbiology > QR74.8 Bacteria
Divisions: Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Muhamad Aditama
Date Deposited: 26 Aug 2021 07:26
Last Modified: 26 Aug 2021 07:26
URI: https://repository.its.ac.id/id/eprint/89754

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