Faizah, Nurul (2023) Enkapsulasi Ekstrak Biji Alpukat dari Metode Ekstraksi Pulse Electric Field sebagai Material Antioksidan, Antibakteri dan Antikanker. Masters thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 6008211024-Master_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
6008211024-Master_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2025. Download (2MB) | Request a copy |
Abstract
Penelitian ini mengembangkan ekstraksi limbah biji alpukat yang kemudian dilanjutkan dengan penerapan teknologi enkapsulasi untuk menjaga stabilitas kandungan senyawa bioaktif tersebut. Penelitian ini bertujuan mempelajari pengaruh electric field strength (E) dan pulse frequency (f) pada ekstraksi dengan metode pulsed electric field (PEF) dan penerapan teknologi enkapsulasi untuk memeroleh partikel terenkapsulasi sebagai material antioksidan, antibakteri dan antikanker. Analisa karakteristik ekstrak biji alpukat (EBA) dan produk enkapsulasi untuk mengetahui performa produk. Kinerja tertinggi proses ekstraksi diperoleh pada 7,5 kV/cm dengan f = 250 Hz dengan yield, total phenolic content (TPC), antioxidant activity (AA), dan IC50 (inhibition concentration ) masing-masing adalah 18,80%, 15,14 mgGAE/g ekstrak, 95,56%, dan 90 µg/mL. Morfologi EBA setelah ekstraksi memiliki lebih banyak pori dan rongga pada sel membran yang terbentuk karena kekuatan medan listrik dan pulse frequency. Analisis GC-MS menunjukkan bahwa ekstrak tersebut mengandung senyawa bioaktif seperti polifenol, asam lemak, dan senyawa lainnya. Spektrum FTIR menunjukkan beberapa puncak yang merepresentasikan OH, C−H stretch, C=C, −C−H stretch, C−O stretch, dan vibrasi deformasi CH menunjukkan adanya antioksidan. Selanjutnya, EBA menunjukkan adanya zona hambat pada pertumbuhan mikroba pada pengamatan hari pertama dan relatif stabil hingga hari ketujuh. Hasil mikrokapsul dari proses spray drying memberikan nilai AA dan aktivitas antibakteri yang stabil. Analisa sitotoksik menunjukkan bahwa EBA memiliki sifat antibakteri terhadap sel kanker kulit.
======================================================================================================================================
Avocado seed as an agricultural by-product rich in bioactive compounds. This research maximizes the extraction of avocado seed waste and followed by the application of encapsulation technology to maintain the stability of the content of these bioactive compounds. This study aims to study the effect of electric field strength and pulse frequency on extraction using the pulsed electric field method and the application of encapsulation technology to obtain encapsulated particles as antioxidant, antibacterial and antibacterial materials. Analysis of the characteristics of avocado seed extract and encapsulation products was carried out to determine the performance of EBA and microcapsule products. The highest performance of the extraction process was obtained at 7.5 kV/cm with a pulse frequency of 250 Hz with extraction yield, total phenolic content, antioxidant activity, and IC50, respectively, 18.80%, 15.14 mgGAE/g extract , 95.56%, and 90 µg/mL . The morphology of EBA after extraction has more pores and cavities in the cell membrane which are formed due to the electric field strength and pulse frequency. GC-MS analysis showed that the extract contained bioactive compounds such as polyphenols, fatty acids and other compounds. The FTIR spectrum shows several peaks representing OH, C−H stretch, C=C, −C−H stretch, C−O stretch, and CH deformation vibration indicating the presence of antioxidants. Furthermore, EBA showed an inhibition zone on microbial growth on the first day of observation and was relatively stable until the seventh day. The results of the microcapsules from the spray drying process provide AA values and stable antibacterial activity. Cytotoxic analysis showed that EBA has antibacterial properties against skin cancer cells.
Actions (login required)
 |
View Item |