Pengaruh Mediaum Limbah Organik Terhadap Aktivitas Enzim Lipase Dari Isolat Kapang Tanah Wonorejo

AKurniawati, lfia Rahma (2014) Pengaruh Mediaum Limbah Organik Terhadap Aktivitas Enzim Lipase Dari Isolat Kapang Tanah Wonorejo. Other thesis, Insititut Teknologi Sepuluh Nopember.

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Abstract

Meningkatnya akumulasi limbah cair organik yang mengandung lipid di lingkungan perlu ditanggulangi. Penelitian ini bertujuan untuk mengetahui aktivitas enzim yang dihasilkan dari isolat kapang Mortierella sp. T3.G1, Aspergillus niger T2.1, Penicillium sp.1 T4.E3 secara tunggal dan konsorsium dalam mendegradasi lipid pada medium limbah organik yang berbeda. Parameter yang diamati dalam penelitian ini adalah nilai degradasi lipid dan nilai aktivitas enzim lipase. Limbah cair organik yang digunakan adalah limbah cair pencucian ikan, limbah cair tangki septik dan limbah minyak goreng bekas. Data pengaruh medium limbah cair organik terhadap aktivitas enzim lipase dianalisis dengan menggunakan ANOVA oneway dan dilanjutkan dengan uji Duncan. Hasil menunjukkan bahwa medium dan isolat berpengaruh terhadap aktivitas lipase dengan nilai P=0,000. Korelasi nilai aktivitas dan nilai degradasi tertinggi ditunjukkan pada penambahan isolat Aspergillus niger dengan nilai aktivitas lipase sebesar 0,1511 U/mL dan degradasi lipid 83,95% pada limbah minyak goreng bekas, 0,0997 U/mL dan degradasi lipid 58,43% pada limbah pencucian ikan dan aktivitas lipase 0,0697 U/mL dan degradasi lipid 67,36% pada limbah tangki septik.
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Increasing the accumulation of organic waste liquid that containing lipids in the neighborhood need to overcome. This research is aimed to know the activity of an enzyme lipase from mold isolate Mortierella sp. T3.G1, Aspergillus niger T2.1, and Penicillium sp.1 T4.E3 singly and a consortium on different medium liquid waste organic. The parameters that are tested in this research is the degradation of lipid and the activity of an enzyme lipase. Organic waste liquid that used there are processing fish wastewater,septic tank wastewater and used cooking waste oil. The influence data of medium against the activity of an enzyme protease analyzed using Annova One-Way and continued with Duncan test. The result showed that medium and isolates affecting the activity of enzymes lipase with P value=0,000. Correlation of activity value and degradation value indicated that isolate Aspergillus niger is the highest in three organic waste liquid with activity lipase 0,0997 U/mL and degradation lipid 58,43 % on processing fish wastewater, activity lipase 0,1511 U/mL and degradation lipid 83,95 % on used cooking waste oil and lipase 0,0697 U/mL and degradation lipid 67,36 % on septic tank wastewater.

Item Type:	Thesis (Other)
Additional Information:	RSBi 660.634 Kur p-1, 2014
Uncontrolled Keywords:	Limbah Organik, Lipase, Degradasi Lipid, Lipase, Lipid Degradation, Organic Waste,
Subjects:	T Technology > TP Chemical technology > TP248.27.P55 Plant biotechnology.
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	10 Jan 2024 03:59
Last Modified:	10 Jan 2024 03:59
URI:	http://repository.its.ac.id/id/eprint/105435

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