UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara) TERHADAP Streptococcus iniae

KARTIKASARI, INTAN (2014) UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara) TERHADAP Streptococcus iniae. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Lantana camara merupakan salah satu tumbuhan dari famili verbenaceae yang banyak mengandung senyawa metabolit sekunder yaitu alkaloid, flavanoid, saponin dan tannin yang bisa berperan sebagai antibakteri. Streptococcus iniae merupakan salah satu bakteri pathogen ikan yang menyebabkan kelesuan, bisul exopthalmia dan meningoencephalitis. Penelitian ini bertujuan untuk mengetahui konsentrasi efektif ekstrak daun tembelekan yang mampu menghasilkan zona hambat tertinggi serta mengetahui nilai MIC (Minimum Inhibitory Concentration) dan MBC (Minimum Bactericidal Concentration) ekstrak daun tembelekan, penelitian ini menggunakan uji difusi cakram untuk menentukan zona hambat dan uji dilusi untuk menentukan MIC dan MBC Hasil dari penelitian ini adalah konsentrasi efektif ekstrak daun tembelekan (Lantana camara L) yang mampu menghasilkan zona hambat tertinggi adalah 400 mg/ml dengan diameter zona hambat 22,00 mm sedangkan nilai MIC dari ekstrak daun adalah 460 mg/ml dan tidak ditemukan nilai MBC pada kisaran konsentrasi 400 mg/ml sampai dengan 580 mg/ml. ============================================================ Lantana camara is one of the family Verbenaceae plant that contain many secondary metabolites which are alkaloids, flavonoids, saponins and tannins that can act as an antibacterial. Streptococcus iniae is one of the bacterial fish pathogen that causes lethargy, ulcers exopthalmia and meningoencephalitis. This study aims to determine the size of the diameter of the inhibition zone formed at each concentration of Lantana camara leaf extract against Streptococcus iniae, determine the minimum concentration of Lantana camara leaf extract that has the potential to inhibit the bacteria Streptococcus iniae, determine the minimum concentration of Lantana camara leaf extract potentially in kill the bacteria Streptococcus iniae. This study using the method disk difution test to measure of inhibition zone and tube dillution test with determination of MIC and MBC The results of this research are the effective concentration of leaf extract tembelekan (Lantana camara ) which is capable to produce the highest inhibition zone was 400 mg / ml with inhibition zone diameter of 22.00 mm while the value of MIC leaf extract is 460 mg / ml and the value of MBC was not found in concentration range of 400 mg/ml until 580 mg/ml

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 579.37 Kar u
Uncontrolled Keywords: Lantana camara, Streptococcus iniae, Zona hambat, MIC,MBC
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Mathematics and Science > Biology > (S1) Undergraduate Theses
Depositing User: Mrs Anis Wulandari
Date Deposited: 10 Jan 2017 02:57
Last Modified: 10 Jan 2017 02:57
URI: http://repository.its.ac.id/id/eprint/1424

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