Gautama, Enrico and Karima, Aulia (2017) Produksi Gula Reduksi dari Sabut Kelapa Menggunakan Kombinasi Teknologi Air Subkritis (dengan Gas Penekan N2) dan Hidrolisis Enzimatik. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Sabut kelapa memiliki kandungan lignoselulosa yang dapat dimanfaatkan menjadi sumber energi. Pada penelitian ini, dilakukan kombinasi pretreatment air subkritis menggunakan gas penekan N2 dan hidrolisis enzimatik untuk meningkatkan dekomposisi kompleks lignoselulosa menjadi gula reduksi. Sabut kelapa diberi pretreatment air subkritis pada reaktor batch dengan variabel tekanan 60, 80, dan 100 bar, suhu 150, 170, dan 190 °C, serta waktu reaksi 20, 40 dan 60 menit. Produk yang dihasilkan dari pretreatment air subkritis yaitu berupa liquid yang mengandung gula dan padatan. Produk padatan kemudian dilanjutkan dengan hidrolisis enzimatik menggunakan campuran enzim selulase dan xilanase. Padatan hasil pretreatment juga dianalisa menggunakan TGA (thermogravimetric analysis) untuk mengetahui stabilitas termal dan analisa SEM (scanning electron microscopy) untuk mengetahui struktur morfologi permukaan padatan. Analisa XRD (x-ray diffraction) dilakukan untuk mengetahui struktur kristalin padatan dan analisa FTIR (fourier transform infrared spectroscopy) dilakukan untuk mempelajari perubahan komposisi kimia dalam padatan. Sedangkan, produk liquid dianalisa menggunakan metode DNS (dinitrosalicylic acid) untuk mendapatkan konsentrasi gula reduksi. Yield gula reduksi tertinggi oleh pretreatment air subkritis didapatkan sebesar 0,146 g/g sabut kelapa yang didapatkan pada kondisi operasi 80 bar, 150 °C dan 40 menit. Pada hidrolisis enzimatik, yield tertinggi dari padatan hasil hidrolisis enzimatik yaitu sebesar 9,91 g/g sabut kelapa pada sampel air subkritis dengan kondisi operasi suhu 150 oC, tekanan 100 bar dan waktu reaksi 40 menit. Sedangkan, berat total gula reduksi tertinggi didapatkan pada variabel 80 bar, 150oC dan 40 menit yaitu sebesar 1,4 g.
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Coconut husk as lignocellulosic biomass can be utilized to produce reducing sugars and then fermented into hydrogen, a potential alternative energy source for the limited fossil fuel. In this study, enzymatic hydrolysis combined with subcritical water pretreatment were used to significantly enhance the decomposition of lignocellulosic complex into simple sugars with several advantages over conventional processes. Coconut husk was pretreated by SCW in a batch reactor at 60, 80 and 100 bar, temperature of 150, 170 and 190 °C, and reaction time of 20, 40 and 60 minutes. The SCW pretreatment produced two products, sugar liquid and solid (SCW-treated substrate). The solid product was continued by enzymatic hydrolysis using mixture of pure cellulase and xylanase enzymes. The substrate was analyzed by TGA (thermogravimetric analysis) to study the thermal stability and SEM (scanning electron microscopy) to study the surface morphology of the substrate. XRD (X-Ray Diffraction) was used to study the crystal structure of the substrate while FTIR (fourier transform infrared spectroscopy) was used to study the changes in chemical composition. The liquid product was analyzed by DNS (dinitrosalicylic acid) method to obtain the concentration of sugar. Maximum yield of reducing sugar by SCW pretreatment was 0.146 g/g raw material obtained at 150 °C and 80 bar for 40 minutes of reaction time. Whereas, maximum yield of reducing sugar by enzymatic hydrolysis was 9.91 g/g raw material obtained at 150 oC and 100 bar for 40 minutes. The highest total mass of reducing sugar obtained at 80 bar, 150 oC and 40 minutes of 1,4 g.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Uncontrolled Keywords: | coconut husk; lignin; lignocellulose; subcritical water; sugar |
| Subjects: | Q Science > QD Chemistry > QD320 Cellulose. Hydrolysis T Technology > TP Chemical technology |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering > 24201-(S1) Undergraduate Thesis |
| Depositing User: | Enrico Gautama |
| Date Deposited: | 25 Sep 2017 07:12 |
| Last Modified: | 06 Mar 2019 03:53 |
| URI: | http://repository.its.ac.id/id/eprint/44282 |
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