Peran Bakteri Bacillus Cereus Dan Pseudomonas Putida Dalam Bioremediasi Logam Berat (Fe, Cu, Dan Zn) Pada Tanah Tercemar Minyak Bumi - The Function Of Bacillus Cereus And Pseudomonas Putida For Heavy Metals (Fe, Cu And Zn) Bioremediation In Petroleum Contaminated Soil

Farida, Annisa Nur and Dalyla, . (2016) Peran Bakteri Bacillus Cereus Dan Pseudomonas Putida Dalam Bioremediasi Logam Berat (Fe, Cu, Dan Zn) Pada Tanah Tercemar Minyak Bumi - The Function Of Bacillus Cereus And Pseudomonas Putida For Heavy Metals (Fe, Cu And Zn) Bioremediation In Petroleum Contaminated Soil. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kegiatan eksplorasi dan produksi minyak bumi sangat berpotensi mencemari lingkungan. Salah satu cara yang dapat dilakukan untuk mengatasi pencemaran minyak bumi adalah dengan metode Bioremediasi yang menggunakan mikroorganisme untuk menghilangkan kandungan logam berat pada minyak bumi yang mencemari tanah. Penelitian ini bertujuan menentukan efisiensi bioremediasi sebagai fungsi konsentrasi logam dengan parameter pengukuran kandungan logam, pH, temperatur dan konsentrasi bakteri pada pengolahan tanah yang terkontaminasi minyak bumi secara bioremediasi ex situ fase slurry bioreaktor. Ada tiga tahapan utama dalam penelitian ini yaitu tahap preparasi tanah dan bakteri, proses bioremediasi dengan bioreaktor, dan analisa konsentrasi logam (Cu, Zn dan Fe). Penelitian ini menggunakan 3 variabel bebas yaitu : konsentrasi mikroba dalam bioreaktor (10% dan 15%), perbandingan jumlah bakteri Bacillus cereus dan Pseudomonas putida yang digunakan (1:0, 1:1, 0:1), serta kandungan logam yang dianalisa (Fe, Cu dan Zn). Pertumbuhan bakteri diamati setiap lima hari dengan cara menghitung jumlah populasi menggunakan hemasitometer. Konsentrasi logam dianalisa dengan metode AAS. Dari hasil iv penelitian, pada variabel 10% bakteri, efisiensi bioremediasi terbesar baik untuk logam Fe, Cu, maupun Zn rata-rata diperoleh menggunakan bakteri B. cereus yakni sebesar 76,67% (Fe), 86,93% (Cu), dan 14,81% (Zn) dengan urutan logam teradsorp dari yang terbesar adalah Cu, Fe dan Zn. Sedangkan pada variabel 15% bakteri, efisiensi bioremediasi terbesar rata-rata diperoleh menggunakan bakteri campuran B.cereus : P.putida = 1:1 yakni sebesar 82,78% (Fe), 100% (Cu), dan 31,84% (Zn) dengan urutan logam teradsorp dari yang terbesar rata-rata adalah Cu, Fe dan Zn. Kemudian untuk efisiensi bioremediasi secara overall lebih bagus menggunakan variabel 15% (v/v) konsentrasi bakteri dalam bioreaktor daripada 10% konsentrasi bakteri. ======================================================================================================================== Exploration and production of crude oil has the potential to pollute the environment. One way that can be done to overcome the oil pollution is the bioremediation method that uses microorganisms to remove the heavy metal content in the petroleum contaminated soil. This study aim to determine the efficiency of bioremediation as a function of the concentration of metal with metal content measurement parameter, pH, temperature and concentration of bacteria in the processing of petroleum contaminated soil. There were three main stages in this research which were soil and bacteria preparation, bioremediation process, and analysis of metal concentrations (Cu, Zn and Fe). This study used three independent variables: the concentration of microbes in the bioreactor (10% and 15%), the ratio of the number of Bacillus cereus and Pseudomonas putida were used (1: 0, 1: 1, 0: 1), and the metal content was analyzed ( Fe, Cu and Zn). Bacterial growth was monitored every five days by counting the number of bacteria using hemocytometer. Metal concentrations were analyzed by AAS method. From the research, the variable of 10% bacteria, the largest bioremediation efficiency for Fe, Cu, and Zn obtained using B. cereus which amounted to 76.67% (Fe), 86.93% (Cu), and 14.81% (Zn) with the sequence adsorbed metal from the largest was Cu, Fe and Zn. While the variable of 15% bacteria, the largest bioremediation efficiency average was obtained using a mixture of bacteria B.cereus: P.putida = 1: 1 which was equal to 82.78% (Fe), 100% (Cu), and 31.84% (Zn) with the sequence of the largest absorbed metal average was Cu, Fe and Zn. Then for the overall efficiency of bioremediation was better to use variable 15% (v/v) concentration of bacteria in the bioreactor than 10% concentration of bacteria.

Item Type: Thesis (Undergraduate)
Additional Information: RSK 628.55 Far p
Uncontrolled Keywords: tanah tercemar minyak bumi, bioremediasi, logam berat, Bacillus cereus, Pseudomonas putida, Petroleum contaminated soil, bioremediation, heavy metals, Bacillus cereus, Pseudomonas putida.
Subjects: S Agriculture > S Agriculture (General) > S593.2 Soil Structure
T Technology > TD Environmental technology. Sanitary engineering > TD878.47 Soil remediation
Divisions: Faculty of Industrial Technology > Chemical Engineering
Depositing User: ansi aflacha
Date Deposited: 06 Dec 2019 03:27
Last Modified: 06 Dec 2019 03:27
URI: http://repository.its.ac.id/id/eprint/72244

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