Farida, Annisa Nur and Dalyla, . (2016) Peran Bakteri Bacillus Cereus Dan Pseudomonas Putida Dalam Bioremediasi Logam Berat (Fe, Cu, Dan Zn) Pada Tanah Tercemar Minyak Bumi - The Function Of Bacillus Cereus And Pseudomonas Putida For Heavy Metals (Fe, Cu And Zn) Bioremediation In Petroleum Contaminated Soil. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Kegiatan eksplorasi dan produksi minyak bumi sangat
berpotensi mencemari lingkungan. Salah satu cara yang dapat
dilakukan untuk mengatasi pencemaran minyak bumi adalah
dengan metode Bioremediasi yang menggunakan mikroorganisme
untuk menghilangkan kandungan logam berat pada minyak bumi
yang mencemari tanah. Penelitian ini bertujuan menentukan
efisiensi bioremediasi sebagai fungsi konsentrasi logam dengan
parameter pengukuran kandungan logam, pH, temperatur dan
konsentrasi bakteri pada pengolahan tanah yang terkontaminasi
minyak bumi secara bioremediasi ex situ fase slurry bioreaktor.
Ada tiga tahapan utama dalam penelitian ini yaitu tahap preparasi
tanah dan bakteri, proses bioremediasi dengan bioreaktor, dan
analisa konsentrasi logam (Cu, Zn dan Fe). Penelitian ini
menggunakan 3 variabel bebas yaitu : konsentrasi mikroba dalam
bioreaktor (10% dan 15%), perbandingan jumlah bakteri Bacillus
cereus dan Pseudomonas putida yang digunakan (1:0, 1:1, 0:1),
serta kandungan logam yang dianalisa (Fe, Cu dan Zn).
Pertumbuhan bakteri diamati setiap lima hari dengan cara
menghitung jumlah populasi menggunakan hemasitometer.
Konsentrasi logam dianalisa dengan metode AAS. Dari hasil
iv
penelitian, pada variabel 10% bakteri, efisiensi bioremediasi
terbesar baik untuk logam Fe, Cu, maupun Zn rata-rata diperoleh
menggunakan bakteri B. cereus yakni sebesar 76,67% (Fe),
86,93% (Cu), dan 14,81% (Zn) dengan urutan logam teradsorp
dari yang terbesar adalah Cu, Fe dan Zn. Sedangkan pada variabel
15% bakteri, efisiensi bioremediasi terbesar rata-rata diperoleh
menggunakan bakteri campuran B.cereus : P.putida = 1:1 yakni
sebesar 82,78% (Fe), 100% (Cu), dan 31,84% (Zn) dengan urutan
logam teradsorp dari yang terbesar rata-rata adalah Cu, Fe dan
Zn. Kemudian untuk efisiensi bioremediasi secara overall lebih
bagus menggunakan variabel 15% (v/v) konsentrasi bakteri dalam
bioreaktor daripada 10% konsentrasi bakteri.
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Exploration and production of crude oil has the potential to
pollute the environment. One way that can be done to overcome
the oil pollution is the bioremediation method that uses
microorganisms to remove the heavy metal content in the
petroleum contaminated soil. This study aim to determine the
efficiency of bioremediation as a function of the concentration of
metal with metal content measurement parameter, pH,
temperature and concentration of bacteria in the processing of
petroleum contaminated soil. There were three main stages in this
research which were soil and bacteria preparation, bioremediation
process, and analysis of metal concentrations (Cu, Zn and Fe).
This study used three independent variables: the concentration of
microbes in the bioreactor (10% and 15%), the ratio of the
number of Bacillus cereus and Pseudomonas putida were used (1:
0, 1: 1, 0: 1), and the metal content was analyzed ( Fe, Cu and
Zn). Bacterial growth was monitored every five days by counting
the number of bacteria using hemocytometer. Metal
concentrations were analyzed by AAS method. From the
research, the variable of 10% bacteria, the largest bioremediation
efficiency for Fe, Cu, and Zn obtained using B. cereus which
amounted to 76.67% (Fe), 86.93% (Cu), and 14.81% (Zn) with
the sequence adsorbed metal from the largest was Cu, Fe and Zn.
While the variable of 15% bacteria, the largest bioremediation efficiency average was obtained using a mixture of bacteria
B.cereus: P.putida = 1: 1 which was equal to 82.78% (Fe), 100%
(Cu), and 31.84% (Zn) with the sequence of the largest absorbed
metal average was Cu, Fe and Zn. Then for the overall efficiency
of bioremediation was better to use variable 15% (v/v)
concentration of bacteria in the bioreactor than 10% concentration
of bacteria.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSK 628.55 Far p |
| Uncontrolled Keywords: | tanah tercemar minyak bumi, bioremediasi, logam berat, Bacillus cereus, Pseudomonas putida, Petroleum contaminated soil, bioremediation, heavy metals, Bacillus cereus, Pseudomonas putida. |
| Subjects: | S Agriculture > S Agriculture (General) > S593.2 Soil Structure T Technology > TD Environmental technology. Sanitary engineering > TD878.47 Soil remediation |
| Divisions: | Faculty of Industrial Technology > Chemical Engineering |
| Depositing User: | ansi aflacha |
| Date Deposited: | 06 Dec 2019 03:27 |
| Last Modified: | 06 Dec 2019 03:27 |
| URI: | http://repository.its.ac.id/id/eprint/72244 |
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