Degradasi Pewarna Azo Merah (Congo Red) Oleh Kapang Wonorejo

Saelan, Latifah Amaliah Binti (2015) Degradasi Pewarna Azo Merah (Congo Red) Oleh Kapang Wonorejo. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Industri tekstil merupakan salah satu industri yang menghasilkan limbah pewarna dengan volume yang besar. Salah satu jenis pewarna yang sering digunakan adalah pewarna azo. Pewarna azo mengandung struktur kompleks aromatik yang stabil dan tidak mudah didegradasi. Salah satu proses degaradasi yang dapat dilakukan yaitu secara biodegradasi dengan memanfaatkan kemampuan makhluk hidup seperti kapang. Kapang yang digunakan sebelumnya telah diketahui dapat mendegradasi pewarna azo Orange II. Sejumlah 21 isolat kapang koleksi Laboratorium Mikrobiologi dan Bioteknologi Jurusan Biologi ITS yang telah diisolasi dari kawasan mangrove Wonorejo digunakan untuk mengetahui potensi isolat tersebut dalam mendegradasi jenis pewarna azo merah (congo red) sebanyak 50 ppm dengan perlakuan tiga pH berbeda yakni pH 4, 5 dan 6. Potensi degradasi isolat kapang akan dilihat pada inkubasi hari ke-7 dan ke-14. Besar nilai konsentrasi degradasi diketahui dari nilai absorbasi pada panjang gelombang 500 nm yang telah dimasukkan ke dalam persamaan pada kurva standar masingmasing pH. Uji degradasi oleh kapang menghasilkan nilai degradasi pada pH 4 sebesar 14% oleh isolat Gliomastix sp. (LM 1020), pada pH 5 sebesar 12,44% oleh isolat Gliocladium sp. (LM 1019), dan pada pH 6 sebesar 12,25% oleh isolat Exophiala sp. (LM 1017) Hasil penelitian menunjukkan bahwa 21 isolat kapang koleksi Laboratorium Mikrobiologi dan Bioteknologi Jurusan viii Biologi ITS dapat mendegradasi pewarna congo red dengan nilai degradasi yang kurang dari 15%. ================================================================================================= The textile industry is one of the industries that produce the waste dye with a large volume. One type of dye that is often used is azo dyes. Azo dyes containing aromatic complex structure that is stable and not easily degraded. One degaradation process that can be done is by biodegradation, which is by leveraging the capabilities of living beings such as fungi. Fungi that was previously used found to degrade azo dye Orange II. Some 21 molds collection of the Laboratory of Microbiology and Biotechnology Department of Biology ITS which has been isolated from the mangrove areas Wonorejo used to determine the potential of these isolates to degrade kind of red azo dyes (congo red) of 50 ppm by treatment with three different pH ie pH 4, 5 and 6. The potential degradation of mold isolates will be seen on the 7th day of incubation and 14th. Great degradation value known with concentration of absorbtion is a wavelength of 500 nm which has been put into the equation in the standard curve of each pH. The degradation Test by fungi produce by isolate Gliomastix sp. (LM 1020) at a pH value of 4 is 14%, at pH 5 is 12.44% by isolate Gliocladium sp. (LM 1019), and at pH is 6 12.25% by isolate Exophiala sp. (LM 1017). In this study showed that 21 isolates of fungi collection of the Laboratory of Microbiology and Biotechnology Department of Biology, ITS can degrade congo red dye degradation value of less than 15%.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 579.53 Sae d
Uncontrolled Keywords: Degradasi, Kapang Wonorejo, Pewarna Azo.
Subjects: Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Yeni Anita Gonti
Date Deposited: 11 Feb 2020 07:49
Last Modified: 11 Feb 2020 07:49
URI: http://repository.its.ac.id/id/eprint/74811

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