Saelan, Latifah Amaliah Binti (2015) Degradasi Pewarna Azo Merah (Congo Red) Oleh Kapang Wonorejo. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Industri tekstil merupakan salah satu industri yang
menghasilkan limbah pewarna dengan volume yang besar. Salah
satu jenis pewarna yang sering digunakan adalah pewarna azo.
Pewarna azo mengandung struktur kompleks aromatik yang
stabil dan tidak mudah didegradasi. Salah satu proses degaradasi
yang dapat dilakukan yaitu secara biodegradasi dengan
memanfaatkan kemampuan makhluk hidup seperti kapang.
Kapang yang digunakan sebelumnya telah diketahui dapat
mendegradasi pewarna azo Orange II.
Sejumlah 21 isolat kapang koleksi Laboratorium
Mikrobiologi dan Bioteknologi Jurusan Biologi ITS yang telah
diisolasi dari kawasan mangrove Wonorejo digunakan untuk
mengetahui potensi isolat tersebut dalam mendegradasi jenis
pewarna azo merah (congo red) sebanyak 50 ppm dengan
perlakuan tiga pH berbeda yakni pH 4, 5 dan 6. Potensi
degradasi isolat kapang akan dilihat pada inkubasi hari ke-7 dan
ke-14. Besar nilai konsentrasi degradasi diketahui dari nilai
absorbasi pada panjang gelombang 500 nm yang telah
dimasukkan ke dalam persamaan pada kurva standar masingmasing
pH.
Uji degradasi oleh kapang menghasilkan nilai degradasi
pada pH 4 sebesar 14% oleh isolat Gliomastix sp. (LM 1020),
pada pH 5 sebesar 12,44% oleh isolat Gliocladium sp. (LM
1019), dan pada pH 6 sebesar 12,25% oleh isolat Exophiala sp.
(LM 1017) Hasil penelitian menunjukkan bahwa 21 isolat kapang
koleksi Laboratorium Mikrobiologi dan Bioteknologi Jurusan
viii
Biologi ITS dapat mendegradasi pewarna congo red dengan nilai
degradasi yang kurang dari 15%.
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The textile industry is one of the industries that produce the
waste dye with a large volume. One type of dye that is often used
is azo dyes. Azo dyes containing aromatic complex structure that
is stable and not easily degraded. One degaradation process that
can be done is by biodegradation, which is by leveraging the
capabilities of living beings such as fungi. Fungi that was
previously used found to degrade azo dye Orange II.
Some 21 molds collection of the Laboratory of Microbiology
and Biotechnology Department of Biology ITS which has been
isolated from the mangrove areas Wonorejo used to determine the
potential of these isolates to degrade kind of red azo dyes (congo
red) of 50 ppm by treatment with three different pH ie pH 4, 5
and 6. The potential degradation of mold isolates will be seen on
the 7th day of incubation and 14th. Great degradation value
known with concentration of absorbtion is a wavelength of 500
nm which has been put into the equation in the standard curve of
each pH.
The degradation Test by fungi produce by isolate Gliomastix
sp. (LM 1020) at a pH value of 4 is 14%, at pH 5 is 12.44% by
isolate Gliocladium sp. (LM 1019), and at pH is 6 12.25% by
isolate Exophiala sp. (LM 1017). In this study showed that 21
isolates of fungi collection of the Laboratory of Microbiology and
Biotechnology Department of Biology, ITS can degrade congo
red dye degradation value of less than 15%.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 579.53 Sae d |
| Uncontrolled Keywords: | Degradasi, Kapang Wonorejo, Pewarna Azo. |
| Subjects: | Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yeni Anita Gonti |
| Date Deposited: | 11 Feb 2020 07:49 |
| Last Modified: | 11 Feb 2020 07:49 |
| URI: | http://repository.its.ac.id/id/eprint/74811 |
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