Potensi Bacillus Sebagai Bakteri Siderofor Dan Bioremoval Logam Besi (Fe)

Farisna, Septa Tri (2015) Potensi Bacillus Sebagai Bakteri Siderofor Dan Bioremoval Logam Besi (Fe). Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Beberapa strain Bacillus mampu menghasilkan siderofor. Siderofor adalah senyawa pengkelat logam besi sebagai respon kekurangan besi. Bacillus juga berpotensi sebagai bioremoval logam besi. Tujuan dari penelitian ini adalah mendapatkan Bacillus siderofor yang resisten logam besi dan mengetahui kemampuan bioremovalnya. Isolat bakteri yang digunakan adalah Bacillus endogenik Kalimas Surabaya. Penapisan bakteri siderofor pada medium Fe- CAS Agar. Uji resistensi dilakukan pada media nutrient agar- FeCl3.6H2O dimulai dari 5 mg/L dan seterusnya. Uji bioremoval besi dilakukan pada konsentrasi 9,496; 33,365; dan 33,594 mg/L. Uji viabilitas dilakukan dengan menumbuhkan isolat yang telah terpapar besi pada medium nutrient agar tanpa FeCl3.6H2O. Bacillus A6, DA11, dan SS19 dapat menghasilkan siderofor dan resisten Fe3+ 33,594 mg/L. Bacillus DA11 dapat meremoval Fe3+ paling tinggi, yaitu 27,967 mg/L pada konsentrasi 33,594 mg/L dengan efisiensi bioremoval 83%. Semua Bacillus mempunyai daya hidup setelah terpapar logam Fe dengan CFU ≥ 300. ================================================================================================= Some Bacillus strains can produce siderophore. Siderophore is a chelating agent for ferric iron as a response to low iron environment. Bacillus also has potency as iron bioremoval. The aim of this research is to get siderophore Bacillus strain which can resist to iron and to know the ability of its bioremoval. This research used Bacillus isolated from Kalimas Surabaya ie: A6, DA11, S1, S6, and SS19. The strains were screened for siderophore bacteria in Fe-CAS Agar medium. Resistance test used nutrient agar-FeCl3.6H2O in 5 mg/L and so on. Ferric bioreduction test was applied in 9,496; 33,365; and 33,594 mg/L. The strains from Ferric bioremoval test were grown in nutrient agar medium without FeCl3.6H2O. Bacillus A6, DA11, and SS19 could produce siderophore and also resisted to 33,594 mg/L Fe3+. Bacillus DA11 had the highest ability of Ferric bioreduction, which was 27,967 mg/L from 33,594 mg/L Ferric with efficiency 83%. All Bacillus had high viability after growing in Fe containing medium with CFU ≥300.

Item Type: Thesis (Undergraduate)
Additional Information: RSBi 579.362 Far p
Uncontrolled Keywords: Bacillus, Bioremoval, FeCl3.6H2O, Siderofor
Subjects: Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology
Divisions: Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Yeni Anita Gonti
Date Deposited: 12 Feb 2020 03:25
Last Modified: 12 Feb 2020 03:25
URI: http://repository.its.ac.id/id/eprint/74825

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