Farisna, Septa Tri (2015) Potensi Bacillus Sebagai Bakteri Siderofor Dan Bioremoval Logam Besi (Fe). Undergraduate thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Beberapa strain Bacillus mampu menghasilkan siderofor.
Siderofor adalah senyawa pengkelat logam besi sebagai respon
kekurangan besi. Bacillus juga berpotensi sebagai bioremoval
logam besi. Tujuan dari penelitian ini adalah mendapatkan
Bacillus siderofor yang resisten logam besi dan mengetahui
kemampuan bioremovalnya.
Isolat bakteri yang digunakan adalah Bacillus endogenik
Kalimas Surabaya. Penapisan bakteri siderofor pada medium Fe-
CAS Agar. Uji resistensi dilakukan pada media nutrient agar-
FeCl3.6H2O dimulai dari 5 mg/L dan seterusnya. Uji bioremoval
besi dilakukan pada konsentrasi 9,496; 33,365; dan 33,594 mg/L.
Uji viabilitas dilakukan dengan menumbuhkan isolat yang telah
terpapar besi pada medium nutrient agar tanpa FeCl3.6H2O.
Bacillus A6, DA11, dan SS19 dapat menghasilkan siderofor
dan resisten Fe3+ 33,594 mg/L. Bacillus DA11 dapat meremoval
Fe3+ paling tinggi, yaitu 27,967 mg/L pada konsentrasi 33,594
mg/L dengan efisiensi bioremoval 83%. Semua Bacillus
mempunyai daya hidup setelah terpapar logam Fe dengan CFU ≥
300.
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Some Bacillus strains can produce siderophore. Siderophore
is a chelating agent for ferric iron as a response to low iron
environment. Bacillus also has potency as iron bioremoval. The
aim of this research is to get siderophore Bacillus strain which
can resist to iron and to know the ability of its bioremoval.
This research used Bacillus isolated from Kalimas Surabaya
ie: A6, DA11, S1, S6, and SS19. The strains were screened for
siderophore bacteria in Fe-CAS Agar medium. Resistance test
used nutrient agar-FeCl3.6H2O in 5 mg/L and so on. Ferric
bioreduction test was applied in 9,496; 33,365; and 33,594 mg/L.
The strains from Ferric bioremoval test were grown in nutrient
agar medium without FeCl3.6H2O.
Bacillus A6, DA11, and SS19 could produce siderophore and
also resisted to 33,594 mg/L Fe3+. Bacillus DA11 had the highest
ability of Ferric bioreduction, which was 27,967 mg/L from
33,594 mg/L Ferric with efficiency 83%. All Bacillus had high
viability after growing in Fe containing medium with CFU
≥300.
| Item Type: | Thesis (Undergraduate) |
|---|---|
| Additional Information: | RSBi 579.362 Far p |
| Uncontrolled Keywords: | Bacillus, Bioremoval, FeCl3.6H2O, Siderofor |
| Subjects: | Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Yeni Anita Gonti |
| Date Deposited: | 12 Feb 2020 03:25 |
| Last Modified: | 12 Feb 2020 03:25 |
| URI: | http://repository.its.ac.id/id/eprint/74825 |
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