Kajian Literatur Keanekaragaman Bacillus spp. Penghasil Keratinase dengan Pendekatan Analisis Molekuler 16S rRNA

Putri, Salma Arizka (2020) Kajian Literatur Keanekaragaman Bacillus spp. Penghasil Keratinase dengan Pendekatan Analisis Molekuler 16S rRNA. Undergraduate thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Keratin merupakan protein yang sulit didegradasi karena mempunyai ikatan sistein disulfida, hidrogen, dan interaksi hidrofobik. Keratinase banyak dimanfaatkan industri pakan ternak, beberapa bakteri mampu menghasilkan keratinase sehingga dapat digunakan sebagai alternatif bioteknologi di industri pakan ternak. Bulu ayam merupakan limbah yang mengandung keratin, dengan memanfaatkan Bacillus sebagai penghasil keratinase, maka bulu ayam dapat dijadikan ransum pakan yang berkualitas. Bacillus memiliki anggota spesies yang cukup beragam dan keanekaragamannya dapat dianalisis menggunakan marka gen 16S rRNA. Studi literatur ini membahas tentang keanekaragaman Bacillus spp. penghasil keratinase melalui komparasi sekuens 16S rRNA dengan database pada National Center for Biotechnology Information (NCBI). Studi ini juga akan menelaah secara detail aktivitas keratinolitik dari beberapa spesies Bacillus tersebut berdasarkan sumber pustaka ilmiah internasional yang bereputasi.
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Keratin is a hard-to-degrade protein because it has cysteine disulfide bonds, hydrogen, and hydrophobic interactions. Keratinase is widely used by the animal feed industry, some bacteria can produce keratinase, which can be used as an alternative in the biotechnology of the animal feed industry. Chicken feathers are wastes that contain keratin, by utilizing Bacillus as a producer of keratinase, the chicken feathers can be used as a quality feed. Bacillus has quite a variety of species members and its diversity can be analyzed using the 16S rRNA gene marker. This literature study discussed the diversity of keratinase-producing Bacillus spp. through comparisons of 16S rRNA sequences based on the database on the National Center for Biotechnology Information (NCBI). This study also analyzed the keratinolytic activity of some of the Bacillus species in detail based on reputable international scientific literature sources.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	16S rRNA, Bacillus spp., filogenetik, keratinase 16S rRNA, Bacillus spp., keratinase, phylogenetic
Subjects:	Q Science Q Science > QH Biology Q Science > QP Physiology > QP624 Molecular biology. Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Salma Arizka Putri
Date Deposited:	25 Aug 2020 06:27
Last Modified:	25 Aug 2020 06:27
URI:	http://repository.its.ac.id/id/eprint/80937

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