Asriana, Andi Nina (2016) Penggunaan Minyak Biji Johar (Senna Seamea Lam) Sebagai Minyak Immersi Dan Zat Pewarna Untuk Identifikasi Bakteri. Masters thesis, Institut Teknologi Sepuluh Nopember Surabaya.
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Abstract
Pada penelitian ini, telah dibuat minyak dari biji tanaman johar (Senna seamea
Lam) dengan metode ekstraksi menggunakan n-Heksana dan dilanjutkan dengan
pemurnian. Biji johar memiliki kadar minyak rendah yakni 13,6%. Minyak yang dihasilkan
berwarna kuning kehijauan, dengan indeks bias tinggi (1,44), viskositas bilangan asam dan
bilangan peroksida yang rendah (44,9 cp; 16,66 mg KOH/g minyak dan 5,54 meq O2/1000
g minyak). Hasil pengamatan menunjukkan minyak biji johar mampu mewarnai bakteri
Gram negatif dan bakteri tahan asam tapi tidak untuk bakteri Gram positif. Pewarnaan
menggunakan minyak biji johar tergolong pewarnaan sederhana
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Johar (Senna seamea Lam) seed oil has been made by n- Hexan solution extraction
and refining afterward. Johar seed contained a poor of oil i.e. 13,6%. This oil has a yellowgreen
colour,
with
high
index
refractive
(1,41)
and
lower
viscosity,
acid
value
and
peroxide
value
(44,9
cp;
16,66
mg
KOH/g
of
oil
dan
5,54
meq
O2/1000 g
of
oil).
The
results
showed
ability
of
johar
seed
oil
to
Gram-negative
bacteria
and
acid-fast
bacteria
staining
but
not
for
Gram-positive
bacteria
staining.
Johar
seed
oil
staining
method
included
in
the
modest
stain.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTKi 595.764 8 Asr p |
| Uncontrolled Keywords: | bakteri, minyak, staining, johar, fisika-kimia |
| Subjects: | Q Science > QR Microbiology > QR74.8 Bacteria T Technology > TP Chemical technology > TP669 Oils, fats, and waxes |
| Divisions: | Faculty of Mathematics and Science > Chemistry > 47101-(S2) Master Thesis |
| Depositing User: | EKO BUDI RAHARJO |
| Date Deposited: | 04 Sep 2020 02:31 |
| Last Modified: | 04 Sep 2020 02:31 |
| URI: | http://repository.its.ac.id/id/eprint/81765 |
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