Pengaruh Jenis Kultivar Melon (Cucumis melo L.) dan Usia Kematangan Buah Terhadap Total Karotenoid dan Ekspresi Gen-Gen Biosintesis Karotenoid

Putri, Deva Permata (2024) Pengaruh Jenis Kultivar Melon (Cucumis melo L.) dan Usia Kematangan Buah Terhadap Total Karotenoid dan Ekspresi Gen-Gen Biosintesis Karotenoid. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Melon merupakan buah komoditas yang mengalami kenaikan produksi hingga 118.711 ton pada tahun 2022. Sistem pengelolaan masa panen dan simpan buah harus diperhatikan untuk mempertahankan nilai ekonomi dan kandungan gizi buah. Buah yang matang ditandai dengan perubahan warna dari hijau menjadi kuning atau merah akibat peningkatan karotenoid. Peningkatan karotenoid dipengaruhi oleh ekspresi gen terkait biosintesis karotenoid, diantaranya adalah CmPSY, CmCRTISO, dan CmLCYB. Penelitian ini menggunakan tiga jenis kultivar melon, yaitu ‘Melona’, ‘Gama Melon Parfum’, dan ‘Tacapa Gold’ dengan tiga usia kematangan yang berbeda yaitu 15, 35, dan 55 DAA (Days After Anthesis). Tujuan dilakukannya penelitian ini adalah untuk mengetahui pengaruh jenis kultivar dan usia kematangan buah terhadap kandungan total karotenoid dan bagaimana ekspresi dari gen-gen biosintesis karotenoid. Total karotenoid ditentukan berdasarkan usia dan jenis kultivar buah, dan dianalisis menggunakan spektrofotometer. Ekspresi gen yang berkaitan dengan biosintesis karotenoid dianalisis menggunakan Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR). Hasil analisis menunjukkan jenis kultivar dan usia kematangan buah berpengaruh nyata terhadap kandungan total karotenoid pada buah, paling tinggi yaitu ‘Melona’ (2,497 ± 0,033 gr/mL FW), diikuti ‘Tacapa Gold’ (0,906 ± 0,002 gr/mL FW), dan ‘Gama Melon Parfum’ Parfum’ (0,841 ± 0,015 gr/mL FW). Ekspresi gen-gen biosintesis karotenoid (CmPSY, CmCRTISO, dan CmLCYB) mengalami peningkatan seiring usia kematangan buah pada semua jenis kultivar. Ekspresi gen-gen tersebut mengalami peningkatan rata-rata pada ‘Melona’, ‘Tacapa Gold’, dan ‘Gama Melon Parfum’ usia 55 DAA berturut-turut sebesar 8,526; 4,579; 4,439 fold dibandingkan pada usia 15 DAA.
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Melon is a commodity fruit that has increased production to 118,711 tons in 2022. The management system for harvesting and storing the fruit must be considered to maintain the economic value and nutritional content of the fruit. Ripe fruit is characterized by a change in color from green to yellow or red due to an increase in carotenoids. Carotenoid increase is influenced by the expression of genes related to carotenoid biosynthesis, including CmPSY, CmCRTISO, and CmLCYB. This study used three types of melon cultivars, namely 'Melona', 'Gama Melon Parfum', and 'Tacapa Gold' with three different ages of maturity namely 15, 35, and 55 DAA (Days After Anthesis). The purpose of this study was to determine the effect of cultivar type and age of fruit maturity on total carotenoid content and the expression of carotenoid biosynthesis genes. Total carotenoids were determined based on the age and type of fruit cultivar, and analyzed using a spectrophotometer. Expression of genes related to carotenoid biosynthesis was analyzed using Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR). The analysis showed that cultivar type and age of fruit maturity significantly influenced the total carotenoid content of the fruit, the highest being 'Melona' (2.497 ± 0.033 gr/mL FW), followed by 'Tacapa Gold' (0.906 ± 0.002 gr/mL FW), and 'Gama Melon Parfum' (0.841 ± 0.015 gr/mL FW). The expression of carotenoid biosynthesis genes (CmPSY, CmCRTISO, and CmLCYB) increased with the age of fruit maturity in all cultivars. The expression of these genes increased on average in 'Melona', 'Tacapa Gold', and 'Gama Melon Parfum' at age 55 DAA by 8.526; 4.579; 4.439 fold compared to age 15 DAA, respectively.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Carotenoid, Gama Melon Parfume, Gene Expression, Melona, Tacapa Gold, Karotenoid, Ekspresi Gen
Subjects:	Q Science Q Science > QK Botany Q Science > QK Botany > QK710 Plant physiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Deva Permata Putri
Date Deposited:	05 Jul 2024 02:10
Last Modified:	05 Jul 2024 02:10
URI:	http://repository.its.ac.id/id/eprint/108140

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