Millah, Hadyata Karima (2024) Optimasi Biodekolorisasi Metil Oranye Oleh Pseudomonas aeruginosa Terimobilisasi pada Matriks SA-PVA-Bentonit dengan Response Surface Methodology (RSM). Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Metil Oranye (MO) merupakan pewarna azo anionik yang banyak digunakan dalam industri tekstil. Limbah pewarna MO berpotensi mengancam kesehatan manusia karena sifatnya yang beracun. Penghilangan MO dapat dilakukan dengan menggunakan bakteri Pseudomonas aeruginosa (PA) yang diimobilisasi dalam matriks SA-PVA-Bentonit dengan perbandingan komposisi 6:4:1 (% b/v). Kondisi optimum dekolorisasi MO yang belum diketahui telah diteliti dengan menggunakan response surface methodology (RSM). Optimasi oleh RSM dilakukan dengan variabel independen meliputi pH (4, 7, dan 10), suhu (30, 37.5, dan 45ºC), dan waktu inkubasi (1, 7, dan 11 hari) dengan %dekolorisasi sebagai variabel dependen (Y1) untuk mengetahui kondisi optimal dan pengaruh variabel independen terhadap dekolorisasi MO. Dekolorisasi optimal dicapai pada pH 6,64, suhu 35,083°C, dan waktu inkubasi 7,6 hari dengan prediksi %dekolorisasi sebesar 89,217%. Kinerja beads dalam kondisi optimal dievaluasi melalui uji penggunaan kembali selama beberapa siklus, dengan hasil secara berurutan sebesar 89,11; 90,6; 78,7; 90,23; dan 89,48%. Aplikasi bakteri PA yang diimobilisasi dalam matriks SA-PVA-Bentonit sebagai agen biodekolorisasi terbukti mampu menghilangkan pewarna MO secara efektif
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Methyl orange (MO) is an anionic azo dye widely used in the textile industry. MO dye waste
has the potential to threaten human health due to its toxic nature. MO removal can be done by
using Pseudomonas aeruginosa (PA) bacterium immobilized in SA-PVA-Bentonite matrix
with a composition ratio of 6:4:1 (% w/v). The unknown optimum conditions for MO
decolorization have been investigated using response surface methodology (RSM).
Optimization by RSM is carried out with independent variables including pH (4, 7, and 10),
temperature (30, 37.5, and 45 ºC), and incubation time (1, 6, and 11 days) with
%decolorization as dependent variable (Y1) to determine the optimal conditions and the
influence of independent variables on MO decolorization. Optimal decolorization was
achieved at pH 6.64, temperature 35.083°C, and incubation time 7.6 days with predicted
%decolorization of 89.217%. The performance of the beads under optimal conditions was
evaluated through reusability tests over several cycles, with results of 89.11, 90.6, 78.7, 90.23,
and 89.48%. The use of immobilized PA bacteria in SA-PVA-Bentonite matrix as
biodecolorization agent was shown to be able to effectively remove MO dye.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biodekolorisasi, Imobilisasi, Metil Oranye, Pseudomonas aeruginosa, RSM, Biodecolorization, Immobilization, Methyl Orange, Pseudomonas aeruginosa, RSM |
| Subjects: | Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Hadyata Karima Millah |
| Date Deposited: | 22 Oct 2024 06:40 |
| Last Modified: | 23 Jun 2025 02:50 |
| URI: | http://repository.its.ac.id/id/eprint/111758 |
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