Isolasi dan Identifikasi Protista dari Serasah Mangrove Wonorejo yang Berpotensi Menghasilkan Polyunsaturated Fatty Acid (PUFA)

Abdurrahman, Muhammad Hisyam (2024) Isolasi dan Identifikasi Protista dari Serasah Mangrove Wonorejo yang Berpotensi Menghasilkan Polyunsaturated Fatty Acid (PUFA). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Thraustochytrid merupakan protista laut penghasil PUFA termasuk DHA. Thraustochytrid
mampu mengakumulasi lipid dalam jumlah yang tinggi terutama golongan omega 3 (DHA) dan
mampu menghasilkan PUFA 30-50% dari total asam lemak dalam sel. Thraustochytrid dapat
ditemukan di daerah ekosistem mangrove termasuk air, sedimen, partikulat detritus daun
mangrove yang jatuh ke lantai ekosistem mangrove. Kawasan mangrove Wonorejo, Surabaya
belum banyak diteliti protista penghasil PUFA sehingga dilakukan penelitian untuk
mendapatkan protista yang berpotensi menghasilkan PUFA. Penelitian ini bertujuan untuk
mengetahui potensi protista penghasil asam lemak tak jenuh (PUFA) di kawasan mangrove
Wonorejo serta mengidentifikasi secara morfologi genus/ spesies protista yang berpotensi
menghasilkan asam lemak tak jenuh (PUFA). Metode yang digunakan adalah isolat protista
diambil dari serasah daun bakau berwarna kuning hingga coklat kehitaman dan kondisi basah
yang jatuh ke lantai ekosistem mangrove di Kawasan mangrove Wonorejo. Isolasi dilakukan
dengan metode direct plating di media GYEP Agar yang mengandung antibiotik dan antifungal.
Protista yang tumbuh akan di purifikasi hingga menghasilkan 1 jenis koloni lalu ditumbuhkan
di media GYEP agar yang telah ditambahkan Triphenyl Tetrazolium Chloride (TTC) untuk
seleksi protista penghasil PUFA. Koloni protista yang berpotensi menghasilkan PUFA akan
berwarna merah dan dilanjutkan dengan uji asam lemak menggunakan kromatografi gas massa
spektrofotometri (GC-MS). Isolat yang menghasilkan PUFA akan diidentifikasi secara
morfologi. Karakteristik morfologi Thraustochytrids terdiri dari bentuk dan ukuran koloni,
dinding sel, formasi zoospora, sel amoeboid, pembelahan binary sel, sel vegetatif, dan sel
zoosporangia. Hasil penelitian terisolasi 2 protista yang berpotensi menghasilkan asam lemak
tak jenuh PUFA (PolyUnsaturated Fatty Acid) dari serasah mangrove Wonorejo. Isolat tersebut
adalah AM2 B yang memiliki PUFA meliputi asam linoleat dan berpotensi menghasilkan
Omega 3 seperti ARA, EPA, dan DHA. Sedangkan Isolat AM3 A memiliki PUFA asam
linoleat. Kedua, spesies protista tersebut teridentifikasi genusnya dengan pendekatan morfologi
adalah protista Thraustochytrids dengan genus Aurantiochytrium

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Thraustochytrid is a marine protist that produces PUFA including DHA. Thraustochytrid
are able to accumulate high amounts of lipids, especially the omega 3 group (DHA) and are
able to produce PUFA 30-50% of the total fatty acids in cells. Thraustochytrid can be found in
mangrove ecosystem areas including water, sediment, mangrove leaf detritus particulates that
fall to the floor of the mangrove ecosystem. The mangrove area of Wonorejo, Surabaya has not
been studied much for protists that produce PUFA, so research was carried out to find protists
that have the potential to produce PUFA. This research aims to determine the potential of
protists producing unsaturated fatty acids (PUFA) in the Wonorejo mangrove area and to
identify morphologically the genus/species of protists that have the potential to produce
unsaturated fatty acids (PUFA). The method used is protist isolates taken from yellow to
blackish brown mangrove leaf litter and wet conditions that fall to the floor of the mangrove
ecosystem in the Wonorejo mangrove area. Isolation was carried out using the direct plating
method on GYEP Agar media which contains antibiotics and antifungals. The protists that grow
will be purified to produce 1 type of colony and then grown on GYEP agar media which has
been added with Triphenyl Tetrazolium Chloride (TTC) for selection of PUFA producing
protists. Protist colonies that have the potential to produce PUFA will be red and followed by a
fatty acid test using gas chromatography mass spectrophotometry (GC-MS). Isolates that
produce PUFA will be identified morphologically. The morphological characteristics of
Thraustochytrids consist of colony shape and size, cell walls, zoospore formation, amoeboid
cells, binary division cells, vegetative cells, and zoosporangia cells. The research results
isolated 2 protists that have the potential to produce PUFA (PolyUnsaturated Fatty Acid) from
the Wonorejo mangrove area. This isolate is AM2 B which has PUFA including linoleic acid
and has the potential to produce Omega 3 such as ARA, EPA and DHA. Meanwhile, isolate
AM3 A has PUFA linoleic acid. Second, the genus of the protist species identified using a
morphological approach is the protist Thraustochytrids with the genus Aurantiochytrium

Item Type:	Thesis (Other)
Uncontrolled Keywords:	PUFA, Protista, Thraustochytrid, TTC, Wonorejo; PUFA, Protist, Thraustochytrid, TTC, Wonorejo
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Muhammad Hisyam Abdurrahman
Date Deposited:	08 Aug 2024 14:08
Last Modified:	08 Aug 2024 14:08
URI:	http://repository.its.ac.id/id/eprint/114279

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