Potensi Crude Kitinase dari Bakteri isolat K8 sebagai Antijamur Ascomycota

Salsabila, Afrilya Dina (2024) Potensi Crude Kitinase dari Bakteri isolat K8 sebagai Antijamur Ascomycota. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 5005201012_Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2026. Download (2MB) | Request a copy

Abstract

Permukan batuan candi yang terpapar oleh lingkungan terbuka menyebabkan pelapukan batuan, salah satunya disebabkan oleh jamur. Telah diketahui dalam penelitian sebelumnya pada cagar budaya candi Brahu dan candi Tikus terdapat dua spesies jamur Ascomycota yang dominan terbukti merusak batuan, yaitu Penicillium oxalicum dan Talaromyces purpureogenus. Upaya pencegahan kerusakan akibat jamur dapat dilakukan secara enzimatis dengan ekstrak kasar (crude) kitinase dari isolat bakteri limbah perikanan. Tujuan dari penelitian ini adalah mengetahui aktivitas crude kitinase bakteri isolat K8 dari limbah perikanan dan potensinya sebagai antijamur untuk beberapa spesies Ascomycota. Penelitian dilakukan dengan memproduksi crude kitinase dari bakteri isolat K8 yang terbukti memiliki kemampuan mendegradasi kitin. Aktivitas crude enzim diukur dengan reagen DNS (asam 3,5- dinitrisalisilat). Kedua spesies jamur Ascomycota dalam media PDB dengan total volume 5 mL diberi perlakuan crude enzim dengan variasi volume enzim yaitu 5 mL, 2,5 mL, dan 0 mL atau tanpa kitinase sebagai kontrol. Uji penghambatan dilakukan secara destruktif selama 7 hari, pengukuran dilakukan pada jam ke 0, 24, 48, 72, 96, 120, 144, dan 168. Hasil penelitian menunjukkan setelah inkubasi 168 jam terjadi penghambatan biomassa jamur P. oxalicum perlakuan kitinase 2,5 mL sebesar 73.61% dan kitinase 5 mL sebesar 80.52%. Penghambatan jamur T. purpureogenus oleh perlakuan kitinase 2,5 mL sebesar 75.06% dan perlakuan kitinase 5 mL sebesar 85.12%. Hasil tersebut menunjukkan perlakuan kitinase 2,5 mL dan 5 mL berbeda nyata dengan kontrol.
==============================================================================================================================
The surface of the temple rocks being exposed to the open environment causes rock weathering, one of which is caused by fungi. In previous research that at the Brahu and Tikus temple cultural reserves there were two dominant species of Ascomycota fungi that were proven to damage rocks, namely Penicillium oxalicum and Talaromyces purpureogenus. Efforts to prevent damage caused by fungi can be done enzymatically with crude chitinase extract from fishery waste bacteria. The aim of this research is to determine the crude chitinase activity of isolate bacteria K8 from fishery waste and its potential as an anti-fungal for several Ascomycota species. The research was carried out by producing crude chitinase from K8 isolate bacteria which was proven to have the ability to degrade chitin. Crude enzyme activity was measured with DNS reagent (3,5-dinitrisalicylic acid). The two species of Ascomycota fungi in PDB media with total volume 5 mL were treated with crude enzyme with varying enzyme volumes, namely 5 mL, 2.5 mL, and 0 mL or without chitinase as a control. The inhibition test was carried out destructively for 7 days, measurements were carried out at 0, 24, 48, 72, 96, 120, 144, and 168 hours. The results showed that after 168 hours of incubation there was inhibition of P. oxalicum fungal biomass treated with chitinase 2.5 mL was 73.61% and 5 mL chitinase was 80.52%. Inhibition of the T. purpureogenus fungus by 2.5 mL chitinase treatment was 75.06% and the 5 mL chitinase treatment was 85.12%. These results showed that the 2.5 mL and 5 mL chitinase treatments were significantly different from the control.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bakteri Kitinolitik, Batuan Candi, Jamur Ascomycota, Kitin, Kitinase, Ascomycota Fungi, Chitin, Chitinase, Chitinolytic Bacteria, Temple Rock
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Afrilya Dina Salsabila
Date Deposited:	12 Aug 2024 03:57
Last Modified:	28 Aug 2024 08:14
URI:	http://repository.its.ac.id/id/eprint/114718

Actions (login required)

View Item