Sintesis Dan Pengembangan Nanofiber Reduced Graphene Oxide/Cuo Sebagai Elektroda Biosensor Glukosa Dengan Metode Electrospinning

Shivany, Ruth Anggristy (2022) Sintesis Dan Pengembangan Nanofiber Reduced Graphene Oxide/Cuo Sebagai Elektroda Biosensor Glukosa Dengan Metode Electrospinning. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penelitian tentang pengembangan sensor glukosa berbasis non-enzimatik mulai banyak dilakukan. Mayoritas sensor glukosa berbasis elektrokimia nonenzimatik bergantung pada respons arus oksidasi glukosa pada permukaan elektroda. Pengeksplorasian bahan-bahan baru sebagai material sensor glukosa terus berkembang. Penelitian ini bertujuan untuk mensintesis nanofiber campuran rGO/CuO sebagai elektroda biosensor dengan metode electrospinning. rGO didapatkan dari proses sintesis kimia dengan metode Hummer. rGO dipilih karena memiliki sifat memperluas permukaan kontak. Adapun CuO dipilih sebagai situs aktif pada elektroda. Nanopartikel rGO/CuO dengan variasi komposisi massa CuO dilarutkan dalam poly-vinyl alkohol (PVA) sebagai linker nanofiber. Metodeelectrospinning dilakukan untuk membentuk nanofiber dengan mengarahkan electrospun ke permukaan fluorine timah oksida (FTO) sehingga dapat digunakan langsung sebagai elektroda kerja. Hasil dari sintesis kemudian dikarakterisasi menggunakan X-Ray Difraction (XRD), Fourier Transform Infra Red (FTIR), Scanning Electron Microscope (SEM), dan pengujian Cyclic Voltammetry (CV). Pengujian sensor juga dilakukan dengan menggunakan Cyclic Voltammetry untuk mengetahui performansi dari elektroda nanofiber rGO/CuO- FTO. Hasil XRD menunjukkan ukuran kristal dari rGO dan CuO dalam komposit dengan penambahan CuO 0,2, 0,4, 0,6 gram masing-masing sebesar 23,11 nm, 26,81 nm, dan 28,37 nm. Pengujian FTIR menunjukkan gugus fungsi yang sesuai untuk komposit rGO/CuO. Hasil SEM menujukkan distribusi rGO dan CuO sebagai komposit dengan CuO menyebar ke setiap permukaaan rGO. Nanofiber rGO/CuO telah dihasilkan dengan ukuran diameter 265 nm. Hasil pengujian menunjukkan dengan semakin besarnya komposisi massa CuO terhadap komposit rGO/CuO dapat memperluas kontak untuk kinerja respon yang baik pada elektroda. Selain itu, elektroda nanofiber rGO/CuO dengan komposisi massa yang besar mendapat performansi yang baik terhadap glukosa, sehingga diperoleh nilai senstivitas sebesar 1,186 mA/mM-1 cm-2 , limit deteksi 0,041 mM dengan rentang linear 1-4 mM.
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The development of a non-enzymatic based glucose sensor has begun in the research. The majority of nonenzymatic electrochemically based glucose sensors rely on the current response of glucose oxidation at the electrode surface. The exploration of new materials as glucose sensor materials continues to grow. This study aims to synthesize nanofiber mixture of rGO/CuO as a biosensor electrode by electrospinning method. rGO was obtained from the chemical synthesis process by the Hummer method. rGO was chosen because it has the property of expanding the contact surface. The CuO was chosen as the active site on the electrode. The rGO/CuO nanoparticles with various mass compositions of CuO were dissolved in poly-vinyl alcohol (PVA) as a nanofiber linker. The electrospinning method is carried out to form nanofibers by directing the electrospun to the surface of fluorine tin oxide (FTO) so that it can be used directly as a working electrode. The results of the synthesis were then characterized uing X-Ray Diffraction (XRD), Fourier Transform Infra Red (FTIR), Scanning Electron Microscope (SEM), and Cyclic Voltammetry (CV) testing. Sensor testing was also carried out using CV to determine the performance of the rGO/CuO-FTO nanofiber electrodes. The XRD results showed the crystal sizes of rGO and CuO in the composite with the addition of CuO 0,2, 0,4, 0,6 grams were 23,11 nm, 26,81 nm, and 28,37 nm, respectively. The FTIR test showed a suitable functional group for the rGO/CuO composite. The SEM results show the distribution of rGO and CuO as a composite with CuO spreading to each surface of the rGO. rGO/CuO nanofibers have been produced with a diameter of 265 nm. The test results show that the greater the mass composition of CuO to the rGO/CuO composite can expand the contact for a good response performance at the electrode. In addition, the rGO/CuO nanofiber electrode with a large mass composition got good performance against glucose, so that a sensitivity value of 1.186 mA/mM1cm-2 was obtained, the detection limit was 0,041 mM with a linear range of 1-4 mM.

Item Type:	Thesis (Other)
Additional Information:	RSMt 677.4 Shi s-1 2022
Uncontrolled Keywords:	Biosensor glukosa, CuO, Nanofiber, rGO, Glucose sensor, Nanofiber
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA418.9.F5 Electrospinning. Nanofibers
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	23 Dec 2024 04:11
Last Modified:	23 Dec 2024 04:11
URI:	http://repository.its.ac.id/id/eprint/116034

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