Fikoremediasi Air Tekontaminasi Logam Berat Pb Dengan Variasi Rasio C:N Oleh Mikroalga Chlorella sp. Dan Dunaliella sp.

Luthfiyah, Al Fatih Fadhilah Jihan (2025) Fikoremediasi Air Tekontaminasi Logam Berat Pb Dengan Variasi Rasio C:N Oleh Mikroalga Chlorella sp. Dan Dunaliella sp. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pada era global industri saat ini ditemukan air tercemar bersumber dari aktivitas pertanian,peternakan,industri,perhotelan yang memiliki nutrien tinggi dan kandungan logam berat Pb mencapai 1 mg/L. Hal ini akan berdampak pada makhluk hidup seperti gangguan saraf, gangguan fisiologis dan kematian. Penerapan teknologi seperti remediasi oleh mikroalga sering diterapkan dalam penyisihan Pb. Akan tetapi perlu dipertimbangkan keseimbangan rasio nutrisi C:N yang terkandung pada media. Nutrisi karbon dan nitrogen digunakan mikroalga untuk kebutuhan sumber energi dan sintetis protein. Penggunaan mikroalga hijau yaitu Chlorella sp. dan Dunaliella sp. adalah solusi yang dipilih dalam penerapan remediasi air tercemar Pb sebesar 76-80%. Jika dibandingkan jenis mikroalga merah dan cokelat yang lebih rendah persen penyisihan Pb. Selain nutrisi, intensitas cahaya sebagai sumber energi fotosintesis untuk pertumbuhan mikroalga pada rentang cahaya 2000 lux hingga 10.000 lux. Uji rasio C:N sangat penting untuk memastikan bahwa kondisi pertumbuhan mikroorganisme optimal dan nutrisi terpenuhi. Variasi rasio C:N yang digunakan penyisihan Pb 1 mg/L adalah 100:7,100:13,100:32 dengan variasi intensitas cahaya yang digunakan 2000,4000,6000 lux pada 100 % Chlorella sp.,100% Dunaliella sp., dan mixed culture. Variabel respon dari percobaan ini adalah penurunan kadar Pb,kepadatan sel mikroalga, pH, suhu,CO2, salinitas, dissolved oxygen, dan bikarbonat selama 14 hari. Kepadatan sel menggunakan hemositometer dan konsentrasi Pb menggunakan Spektrofotometer Serapan Atom. Hasil dari pengujian variasi rasio nutrisi C:N didapatkan rasio C:N seimbang dalam penyisihan Pb untuk single culture mikroalga Chlorella sp. adalah 100:13 intensitas cahaya 6000 lux dengan efisiensi biosorpsi 67,5% dan 100:32 6000 lux dengan nilai 65%. Secara uji statistik tidak signifikan maka tidak ada perbedaan nyata antar dua rasio C:N tersebut terhadap penyisihan Pb oleh mikroalga. Perbedaan 2% tersebut dipengaruhi kondisi salinitas di media yang lebih rendah pada 100:13. Sedangkan pada single culture mikroalga Dunaliella sp. 77% rasio C:N 100:32 intensitas cahaya 6000 lux. Mixed culture dihasilkan penyisihan Pb 51% pada rasio C:N 100:32 6000 lux Perbedaan ini didasari pada strategi penyisihan Pb dan struktur sel dua mikroalga ini berbeda. Hasil analisis varians ANOVA pada Dunaliella sp. dan mixed culture antara rasio C:N dan kepadatan sel adalah signifikan yaitu ada perbedaan nyata secara statistik
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In the current era of global industry, polluted water is found to originate from agricultural, livestock, industrial, and hospitality activities that have high nutrients and Pb heavy metal content reaching 1 mg/L. This will have an impact on living things such as nervous disorders, physiological disorders and death. The application of technologies such as remediation by microalgae is often applied in Pb removal. However, it is necessary to consider the balance of the C:N nutrient ratio contained in the media. Carbon and nitrogen nutrients are used by microalgae for energy sources and protein synthesis needs. The use of green microalgae, namely Chlorella sp. and Dunaliella sp. is the chosen solution in the application of Pb-polluted water remediation of 76-80%. When compared to red and brown microalgae which have a lower Pb removal percentage. In addition to nutrients, light intensity as a source of photosynthetic energy for microalgae growth in the light range of 2000 lux to 10,000 lux. Testing the C:N ratio is very important to ensure that optimal microorganism growth conditions and nutrients are met. The variation of C:N ratio used for Pb removal of 1 mg/L was 100:7, 100:13, 100:32 with variations in light intensity used 2000, 4000, 6000 lux on 100% Chlorella sp., 100% Dunaliella sp., and mixed culture. The response variables of this experiment were the decrease in Pb levels, microalgae cell density, pH, temperature, CO2, salinity, dissolved oxygen, and bicarbonate for 14 days. Cell density was measured using a hemocytometer and Pb concentration using an Atomic Absorption Spectrophotometer. The results of testing variations in the C:N nutrient ratio obtained a balanced C:N ratio in Pb removal for a single culture of Chlorella sp. microalgae were 100:13 light intensity of 6000 lux with a biosorption efficiency of 67.5% and 100:32 6000 lux with a value of 65%. Statistically, there is no significant difference between the two C:N ratios on Pb removal by microalgae. The 2% difference is influenced by the lower salinity conditions in the media at 100:13. While in the single culture of Dunaliella sp. microalgae 77% C:N ratio 100:32 light intensity 6000 lux. Mixed culture produced 51% Pb removal at a C:N ratio of 100:32 6000 lux. This difference is based on the Pb removal strategy and the different cell structures of the two microalgae. The results of the ANOVA analysis of variance on Dunaliella sp. and mixed culture between the C:N ratio and cell density are significant, meaning there is a statistically significant difference

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Chlorella sp., Dunaliella sp., Fikoremediasi,Pb, Rasio C:N Chlorella sp, C:N Ratio, Dunaliella sp, Pb, Phycoremediation
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD192.5 Bioremediation T Technology > TD Environmental technology. Sanitary engineering > TD756.8 Phycoremediation.
Divisions:	Faculty of Civil, Planning, and Geo Engineering (CIVPLAN) > Environmental Engineering > 25101-(S2) Master Thesis
Depositing User:	Al Fatih Fadhilah Jihan Luthfiyah
Date Deposited:	21 Jul 2025 01:25
Last Modified:	21 Jul 2025 01:39
URI:	http://repository.its.ac.id/id/eprint/120033

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