Enzim Kitinase dari Isolat Bakteri Kitinolitik untuk Menghambat Pertumbuhan Jamur yang Diisolasi dari Batuan Candi

Tiara, Salma (2025) Enzim Kitinase dari Isolat Bakteri Kitinolitik untuk Menghambat Pertumbuhan Jamur yang Diisolasi dari Batuan Candi. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pelapukan batuan yang ditemukan di beberapa tempat seperti di Cagar Budaya Candi Borobudur maupun Candi lainnya dapat disebabkan karena adanya interaksi antara jamur dengan batuan tersebut. Kitin yang menyusun dinding sel jamur merupakan biopolimer terbesar kedua di alam setelah selulosa. Pertumbuhan jamur yang tinggi dapat diatasi oleh metode enzimatis. Kitinase adalah enzim yang menghidrolisis kitin dengan mengubah polimer kitin menjadi kitooligosakarida (COS), kitinase dapat dihasilkan dari bakteri kitinolitik yang didapatkan dari cangkang maupun limbah perikanan. Penelitian ini bertujuan untuk mendapatkan crude kitinase dari isolat bakteri kitinolitik CK.12 yang berpotensi menghambat pertumbuhan jamur, mengetahui aktivitas crude kitinase dan mengetahui persentase pengaruh crude kitinase terhadap penghambatan pertumbuhan jamur yang diisolasi dari batuan candi. Dilakukan peremajaan isolat bakteri CK.12 yang selanjutnya dibuat kurva pertumbuhan sehingga dapat diketahui waktu stasionernya saat enzim diproduksi. Kemudian dibuat kultur starter bakteri untuk digunakan sebagai acuan waktu produksi crude enzim. Crude kitinase diperoleh dari ekstraksi bakteri kitinolitik CK.12. Aktivitas enzim kitinase dideteksi dengan reagen DNS (3,5-dinitrosalycilic acid) kemudian diukur dengan spektrofotometer. Aktivitas enzim tertinggi diperoleh setelah 24 jam sebesar 68,34 U/mL. Perlakuan kitinase bervariasi antara 0 (kontrol), 2,5 mL, dan 5 mL per total volume 10 mL. Aplikasi crude enzim kitinase dilakukan pada 2 spesies jamur yaitu Talaromyces purpureogenus dan Penicillium oxalicum secara destruktif selama 168 jam. Hasil penelitian menunjukkan bahwa crude kitinase dari isolat bakteri CK.12 mampu menghambat pertumbuhan jamur. Penghambatan biomassa jamur oleh crude enzim kitinase yang terbesar adalah perlakuan 5 mL dengan penghambatan Talaromyces purpureogenus sebesar 77,84% dan Penicillium oxalicum sebesar 95,31%.
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Weathering of rocks found in several places such as in the Borobudur Temple Cultural Reserve and other temples can be caused by the interaction between fungi and these rocks. The chitin that makes up the fungal cell wall is the second largest biopolymer in nature after cellulose. High fungal growth can be overcome by enzymatic methods. Chitinase is an enzyme that hydrolyzes chitin by converting chitin polymers into chitooligosaccharides (COS), chitinase can be produced from chitinolytic bacteria obtained from shells and fishery waste. This study aims to obtain crude chitinase from chitinolytic bacterial isolate CK.12 which has the potential to inhibit fungal growth, determine the activity of crude chitinase, and determine the percentage effect of crude chitinase on inhibiting fungal growth isolated from temple rocks. Rejuvenation of bacterial isolate CK.12 was carried out, which then made a growth curve so that the stationary time could be known when the enzyme was produced. Then a bacterial starter culture was made to be used as a reference for crude enzyme production time. Crude chitinase was obtained from the extraction of chitinolytic bacteria CK.12. Chitinase enzyme activity was detected with DNS (3,5-dinitrosalycilic acid) reagent and then measured by spectrophotometer. The highest enzyme activity was obtained after 24 hours at 68.34 U/mL. The chitinase treatment varied between 0 (control), 2.5 mL, and 5 mL per total volume of 10 mL. The application of crude chitinase enzyme was conducted on two fungal species, Talaromyces purpureogenus and Penicillium oxalicum destructively for 168 hours. The results showed that crude chitinase from bacterial isolate CK.12 was able to inhibit fungal growth. The greatest inhibition of fungal biomass by crude chitinase enzyme was the 5 mL treatment with inhibition of Talaromyces purpureogenus by 77.84% and Penicillium oxalicum by 95.31%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bakteri Kitinolitik, Batuan Candi, Crude Kitinase, Jamur, Chitin, Crude Chitinase, Fungi, Temple rocks.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Salma Tiara
Date Deposited:	01 Aug 2025 01:10
Last Modified:	01 Aug 2025 01:10
URI:	http://repository.its.ac.id/id/eprint/123903

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