Seleksi In Vitro Kalus Tanaman Padi (Oryza sativa L.) Varietas Jeliteng pada Media NaCl untuk Mendapatkan Varian Tahan Salin

Ngizati, Eka Nurwakhidatul (2025) Seleksi In Vitro Kalus Tanaman Padi (Oryza sativa L.) Varietas Jeliteng pada Media NaCl untuk Mendapatkan Varian Tahan Salin. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Tanaman padi memiliki peran penting dalam perekonomian masyarakat sebagai komoditas pangan utama yang memenuhi kebutuhan dasar negara. Kebutuhan masyarakat akan beras terus meningkat seiring dengan pertumbuhan penduduk tetapi, upaya dalam meningkatkan produksi padi menghadapi kendala. Hal ini disebabkan adanya alih fungsi lahan dan disebabkan oleh faktor abiotik salah satunya adalah salinitas lahan. Dengan demikian, diperlukan varietas yang tahan terhadap kondisi salinitas tinggi salah satunya dengan pendekatan kultur in vitro. Penelitian ini bertujuan untuk menentukan media kultur yang paling optimal untuk pertumbuhan kalus tanaman padi (Oryza sativa L.) dan mengukur ketahanan padi varietas Jeliteng terhadap cekaman salinitas dengan perlakuan NaCl dengan konsentrasi 0, 2.500, 5.000, dan 7.500 ppm. Langkah kerja dalam penelitian ini meliputi induksi kalus, seleksi salinitas, pengamatan yang meliputi parameter warna kalus, tekstur kalus, diameter kalus, berat kalus, dan persentase kalus bertahan, dan analisis data. Berdasarkan hasil penelitian, Penambahan 2,4-D menghasilkan kalus dengan warna putih kekuningan sedangkan pada penambahan NAA dan BAP menghasilkan kalus dengan warna hitam dan bertekstur kompak. Pada media N6 dan N6+NAA 1,5 ppm tidak tumbuh kalus tetapi tumbuh akar. Penambahan ZPT 2,4-D 1 pada media menghasilkan rata-rata tertinggi pada persentase terbentuknya kalus, berat, dan diameter sehingga media 2,4-D 1 merupakan media yang optimal pada tahap induksi kalus. Padi varietas Jeliteng mampu bertahan pada cekaman salinitas tertinggi dengan media NaCl konsentrasi 7.500 ppn dengan persentase kalus yang browning sebesar 5%. ==========================================================================================================================================
Rice plants have an important role in the community’s economy as the main food commodity that fulfills the basic needs of the country. People's need for rice continues to increase along with population growth, but efforts to increase rice production face obstacles. This is due to land conversion and abiotic factors, one of which is land salinity. Thus, varieties that are resistant to high salinity conditions are needed, one of which is the in vitro culture approach. This research aims to determine the most optimal culture media for callus growth of rice plants (Oryza sativa L.) variety Jeliteng and measure the resistance of rice variety Jeliteng to salinity stress with NaCl treatment with concentrations of 0, 2,500, 5,000, and 7,500 ppm. Work steps in this study include callus induction, salinity selection, and observations that include parameters callus color, callus texture, callus diameter, callus weight, and percentage of callus survived, and data analysis.. The work steps in this study include callus induction, salinity selection, observations that include callus color parameters, callus texture, callus diameter, callus weight, and percentage of live callus. Based on the results of the study, the addition of 2,4-D produced callus with a white yellowish color while the addition of NAA and BAP produced callus with a black color and compact texture. On media N6 and N6+NAA 1.5 ppm did not grow callus but grew roots. The addition of ZPT 2,4-D 1 to the media produces the highest average on the percentage of callus formation, weight, and diameter so that 2,4-D 1 media is the optimal media at the callus induction stage. The Jeliteng rice variety is able to withstand the highest salinity stress with a NaCl concentration of 7.500 ppm with a browning callus percentage of 5%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Kalus, Kultur in vitro, NaCl, Salinitas, Tanaman padi Callus, In vitro culture, NaCl, Salinity, Rice plant
Subjects:	Q Science Q Science > QH Biology > QH301 Biology Q Science > QK Botany > QK731 Growth (Plants)
Divisions:	Faculty of Architecture, Design, and Planning > Architecture > 23101-(S2) Master Thesis
Depositing User:	Eka Nurwakhidatul Ngizati
Date Deposited:	01 Aug 2025 03:53
Last Modified:	01 Aug 2025 03:53
URI:	http://repository.its.ac.id/id/eprint/124500

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