Dwitasari, Nabilah Ayu (2025) Analisis Pengaruh Konsentrasi Propolis Dengan Metode Dip Coating Pada Crosslink Glutaraldehyde Terhadap Morfologi, Kuat Tekan, Bioaktivitas, dan Laju Release Pada Bovine Hydroxyapatite. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Untuk memperbaiki properties bovine hydroxyapatite, pemberian crosslinker dapat menjadi salah satu metode yang dapat diterapkan. Glutaraldehyde merupakan agen crosslinker yang umum digunakan pada material bioaktif karena efektif untuk meningkatkan sifat biomekanik dengan cara membentuk ikatan yang kuat dalam matriks. Di sisi lain, penambahan propolis pada material bone graft dapat memberikan sifat antiinflamasi karena mengandung senyawa flavonoid yang dapat meningkatkan sel fibroblas. Penelitian ini bertujuan untuk menganalisis pengaruh konsentrasi propolis dengan crosslinker glutaraldehyde terhadap morfologi, biokompabilitas, kuat tekan, serta laju release propolis pada Bovine Hydroxyapatite (BHA). BHA di-immerse pada larutan glutaraldehyde (0,1 dan 0,5%) selama 24 jam, dilanjut dengan proses dehydrothermal treatment (DHT) pada temperatur 120 °C selama 30 menit. Selanjutnya, BHA/glutaraldehyde diimerse dengan larutan propolis dengan variasi konsentrasi 25, 50, dan 75%(%volume) selama 24 jam dan dikeringkan selama 24 jam pada temperatur 45 °C. Hasil uji tekan scaffold BHA/glutaraldehyde/propolis menunjukkan peningkatan sebesar ± 9 MPa dibandingkan BHA murni. Peningkatan nilai kuat tekan terlihat pada konsentrasi propolis 50% diikuti penurunan pada konsentrasi propolis 75% pada seluruh variasi glutaraldehyde. Hasil porositas yang dianalisis menggunakan SEM juga menunjukkan hasil yang linear dengan hasil uji tekan. Penambahan konsentrasi propolis juga meningkatkan kadar air. Pada uji aktivitas antibakteri, penambahan persentase propolis mampu menghambat pertumbuhan bakteri S. Aureus hingga 25,4 mm pada sampel BHA/P75 namun penambahan konsentrasi glutaraldehyde menurunkan sifat antibakteri scaffold. Di sisi lain, konsentrasi glutaraldehyde pada scaffold BHA/glutaraldehyde/propolis mampu memperlambat difusi propolis dan pelepasan yang lebih terkendali (sustained release) selama 14 hari.
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To improve the properties of bovine hydroxyapatite, the use of a crosslinker can be an effective method. Glutaraldehyde is a commonly used crosslinking agent in bioactive materials because it effectively enhances biomechanical properties by creating strong bonds within the matrix. On the other hand, incorporating propolis into bone graft materials can provide anti-inflammatory properties, as propolis contains flavonoid compounds that can stimulate fibroblast cells. This study aims to analyze the effects of varying concentrations of propolis in combination with the glutaraldehyde crosslinker on the morphology, biocompatibility, compressive strength, and propolis release rate in Bovine Hydroxyapatite (BHA). BHA was immersed in glutaraldehyde solutions (0.1% and 0.5%) for 24 hours, followed by a dehydrothermal treatment (DHT) at 120°C for 30 minutes. The BHA/glutaraldehyde samples were then immersed in propolis solutions at concentrations of 25%, 50%, and 75% (v/v) for 24 hours and subsequently dried at 45°C for 24 hours. The compressive strength test results for the BHA/glutaraldehyde/propolis scaffold showed an increase of approximately 9MPa compared to pure BHA. The highest increase in compressive strength was observed at 50% propolis concentration, followed by a decrease at 75% propolis for all variations of glutaraldehyde. Porosity results analyzed using SEM also showed a linear correlation with the compressive test results. Increasing the concentration of propolis also led to higher water content. In antibacterial activity tests, increasing the percentage of propolis inhibited Staphylococcus aureus growth up to 25.4mm in the BHA/P75 sample. However, increasing the concentration of glutaraldehyde reduced the antibacterial properties of the scaffold. Conversely, the glutaraldehyde concentration in the BHA/glutaraldehyde/propolis scaffold was able to slow down propolis diffusion and achieve more controlled (sustained) release over 14 days.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Bovine Hydroxyapatite, Glutaraldehyde, Propolis, Scaffold, Tissue Engineering, Bovine Hydroxyapatite, Glutaraldehyde, Propolis, Scaffold, Tissue Engineering. |
| Subjects: | R Medicine > RD Surgery > RD755.5.C35 Orthopedic implants. T Technology > T Technology (General) > TA404 Materials--Biodegradation T Technology > TP Chemical technology > TP156.C64 Controlled release technology |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Nabilah Ayu Dwitasari |
| Date Deposited: | 01 Aug 2025 02:16 |
| Last Modified: | 01 Aug 2025 02:16 |
| URI: | http://repository.its.ac.id/id/eprint/124924 |
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