Sintesis, Karakterisasi, Dan Analisis Performa BHA Hasil Modifikasi Permukaan Secara Kimia Dengan Kemampuan Slow Release Protein Secretome

Khoirunnisa, Syahrin Haifa (2025) Sintesis, Karakterisasi, Dan Analisis Performa BHA Hasil Modifikasi Permukaan Secara Kimia Dengan Kemampuan Slow Release Protein Secretome. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penelitian ini bertujuan untuk menganalisis pengaruh penambahan sodium alginate dan PCL (Polycaprolactone), serta pengaruh modifikasi permukaan secara kimia pada scaffold BHA/Alg/PCL terhadap mechanical propertiesnya, laju degradasi, dan kemampuan slow release sekretom untuk aplikasi protein delivery system. Sistem pengantaran protein semakin mendapat perhatian sebagai metode yang efisien dalam mengirimkan protein terapeutik, namun tantangan utama dalam penggunaannya adalah pengendalian laju pelepasan protein dan stabilitas matriks yang digunakan. Dalam penelitian ini penambahan PCL bertujuan untuk meningkatkan kestabilan scaffold dan kontrol terhadap kecepatan pelepasan protein, sementara sodium alginat diharapkan dapat memperlambat degradasi BHA. Variasi komposisi SA yang digunakan yaitu 2, 4, dan 6 wt.% dengan variasi penambahan PCL sebesar 10, 15, dan 20 wt.%. Proses pembuatan scaffold dilakukan dengan teknik dip coating dan karakterisasi dilakukan meliputi compressive strength, wettability untuk menganalisis hidrofilisitas, fourier transform infrared spectroscopy (FTIR) untuk menganalisis komposisi scaffold dan menganalisis pelepasan secretome dari scaffold selama 2 minggu, x-ray diffraction (XRD), dan scanning electron microscopy (SEM) untuk melihat morfologi scaffold. Hasil penelitian menunjukkan bahwa penambahan PCL berpengaruh terhadap sifat mekanik, wettability, porositas dan performa swelling serta degradation scaffold BHA/Alg/PCL. Sedangkan, sodium alginate tidak memiliki pengaruh secara signifikan. Scaffold S4P15 (4% SA dan 15% PCL) memiliki sifat hidrofilik, ukuran porositas 5,003 μm, compressive strength 15,52 ± 3,17 MPa, swelling ratio 11,04 ± 0,84%, weight remaining 10,42 ± 1%, dan viability cell sebesar 81,26 ± 3,81%, yang mana hasil ini mendekati kriteria sebagai scaffold untuk mengatasi kerusakan critical size pada mandibular bone.
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This study aims to analyze the effect of the addition of sodium alginate and PCL (Polycaprolactone), as well as the effect of chemical surface modification on the BHA/Alg/PCL scaffold, on its mechanical properties, degradation rate, and slow-release capability of secretomes for protein delivery system applications. Protein delivery systems have gained increasing attention as an efficient method for delivering therapeutic proteins, but the main challenges in their use are controlling the protein release rate and the stability of the matrix used. In this study, the addition of PCL aims to enhance scaffold stability and control the protein release rate, while sodium alginate is expected to slow down the degradation of BHA. The sodium alginate compositions used were 2, 4, and 6 wt.%, with PCL additions of 10, 15, and 20 wt.%. The scaffold preparation process was conducted using the dip coating technique, and characterization included compressive strength, wettability to analyze hydrophilicity, Fourier Transform Infrared Spectroscopy (FTIR) to analyze the scaffold composition and secretome release from the scaffold over 2 weeks, X-ray diffraction (XRD), and scanning electron microscopy (SEM) to observe the scaffold morphology. The results showed that the addition of PCL influenced the mechanical properties, wettability, porosity, and performance of swelling and degradation of the BHA/Alg/PCL scaffold. Sodium alginate, on the other hand, did not have a significant effect. The S4P15 scaffold (4% SA and 15% PCL) exhibited hydrophilic properties, a porosity size of 5.003 μm, compressive strength of 15.52 ± 3.17 MPa, swelling ratio of 11.04 ± 0.84%, weight remaining of 10.42 ± 1%, and cell viability of 81.26 ± 3.81%, which are close to the criteria for scaffolds to address critical-size damage in mandibular bone.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	PCL, Protein delivery system, Secretome, Slow Release, Sodium Alginate PCL, Protein delivery system, Secretome, Slow Release, Sodium Alginate
Subjects:	R Medicine > R Medicine (General) > R857.M3 Biomedical materials. Biomedical materials--Testing. R Medicine > RD Surgery > RD755.5.C35 Orthopedic implants. R Medicine > RD Surgery > RD755.6 Bone substitutes.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 27101-(S2) Master Thesis
Depositing User:	Syahrin Haifa Khoirunnisa
Date Deposited:	01 Aug 2025 02:27
Last Modified:	01 Aug 2025 02:27
URI:	http://repository.its.ac.id/id/eprint/125004

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