Pratama, M. Mirza Handi (2025) Pengaruh Silicon Nanoparticles (SiNPs) Sebagai Elisitor In Vitro Terhadap Pertumbuhan dan Kandungan Metabolit Sekunder pada Peppermint (Mentha piperita L.). Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Peppermint (Mentha piperita L.) telah secara luas dikenal dengan kandungan metabolit sekundernya yang memiliki beragam manfaat. Kandungan metabolit sekunder peppermint dapat ditingkatkan melalui metode elisitasi menggunakan silicon nanoparticles. Elisitasi merupakan proses induksi sistem pertahanan tanaman guna meningkatkan produksi metabolit sekunder dari tanaman tersebut. Penelitian ini bertujuan mengetahui pengaruh SiNPs secara in vitro terhadap pertumbuhan dan kandungan metabolit sekunder pada peppermint. Penelitian dilakukan dengan penanaman eksplan pada media elisitasi SiNPs (0;50;100 ppm), dilanjutkan pengukuran parameter pertumbuhan, maserasi ekstrak, perhitungan persentase rendemen, pengukuran kadar TPC dan TFC serta analisis GC-MS. Hasil menunjukkan SiNPs 50 ppm secara signifikan meningkatkan pertumbuhan peppermint ditinjau dari rata-rata berat basah (0,38 gram/eksplan), jumlah tunas (3,83 tunas/eksplan), dan jumlah daun (32 daun/eksplan). SiNPs 50 ppm mampu meningkatkan kadar klorofil a, b, total klorofil dan karotenoid peppermint berturut-turut sebesar 28,14; 11,2; 39,34; 8,53 μg/ml. Kadar TPC dan TFC peppermint mengalami peningkatan signifikan berturut-turut sebesar 8,43 mg GAE/g ekstrak dan 37,16 mg QE/g ekstrak pada SiNPs 50 ppm. Persentase rendemen tertinggi sebesar 3,33% dihasilkan oleh SiNPs 100 ppm. Hasil analisis GC-MS menunjukkan SiNPs 50 ppm memiliki profil penyusun yang lebih banyak dibandingkan perlakuan kontrol dengan didominasi oleh kelompok monoterpen (80,95%) sedangkan perlakuan kontrol lebih didominasi oleh kelompok sesquiterpen (50%).
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Peppermint (Mentha piperita L.) is widely known for its secondary metabolites, which have a variety of benefits. The secondary metabolite content of peppermint can be increased through elicitation using silicon nanoparticles. Elicitation is the process of inducing the plant's defense system to increase the production of secondary metabolites from the plant. This study aims to investigate the effects of SiNPs in vitro on the growth and secondary metabolite content of peppermint. The study was conducted by planting explants in SiNP elicitation media (0; 50; 100 ppm), followed by measurements of growth parameters, extraction yield, percentage yield calculation, determination of TPC and TFC levels, and GC-MS analysis. The results showed that SiNPs at 50 ppm significantly increased peppermint growth, as evidenced by the average fresh weight (0.38 grams/explant), number of shoots (3.83 shoots/explant), and number of leaves (32 leaves/explant). SiNPs at 50 ppm were able to increase the levels of chlorophyll a, b, total chlorophyll, and carotenoids in peppermint by 28.14, 11.2, 39.34, and 8.53 μg/ml, respectively. The TPC and TFC levels of peppermint increased significantly by 8.43 mg GAE/g extract and 37.16 mg QE/g extract, respectively, at 50 ppm SiNPs. The highest yield percentage of 3.33% was produced by 100 ppm SiNPs. GC-MS analysis results showed that SiNPs at 50 ppm had a more diverse composition profile compared to the control treatment, dominated by the monoterpene group (80.95%), while the control treatment was more dominated by the sesquiterpene group (50%).
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Elisitasi, in vitro, Peppermint, SiNPs, Metabolit sekunder, Elicitation, In vitro, Peppermint, Secondary Metabolites, SiNPs. |
| Subjects: | Q Science Q Science > QK Botany Q Science > QK Botany > QK14.5 Botanical literature (Including works on herbals) Q Science > QK Botany > QK710 Plant physiology Q Science > QK Botany > QK731 Growth (Plants) S Agriculture > SB Plant culture S Agriculture > SB Plant culture > SB409.58 Plant propagation. Including in vitro propagation |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | M. Mirza Handi Pratama |
| Date Deposited: | 01 Aug 2025 07:26 |
| Last Modified: | 01 Aug 2025 07:26 |
| URI: | http://repository.its.ac.id/id/eprint/125311 |
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