Studi In Vitro Pengaruh Senyawa Trisindolina 5 Terhadap Ekspresi Gen c-Myc dan cyclin D Pada Kultur Sel HeLa

Azzahro, Marifa Nisa (2025) Studi In Vitro Pengaruh Senyawa Trisindolina 5 Terhadap Ekspresi Gen c-Myc dan cyclin D Pada Kultur Sel HeLa. Other thesis, Insitut Teknologi Sepuluh Nopember.

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Abstract

Kanker serviks adalah tumor ganas yang mengancam 10% hingga 40% kesehatan wanita dimana penyebab utamanya karena infeksi dari human papillomavirus (HPV). Salah satu gen yang berperan dalam perkembangan dan proliferasi kanker adalah c-Myc yang dapat menginduksi ekspresi gen cyclin D dalam transisi fase G1 ke fase S pada siklus sel. Pengobatan kanker seperti kemoterapi atau radioterapi masih ditemukan subpopulasi sel HeLa yang berpotensi menghasilkan tumor kembali. Oleh karena itu, perlu dikembangkan pengobatan kanker yang bersifat spesifik dengan memanfaatkan bahan alami seperti Trisindolina yang merupakan senyawa turunan indol yang diisolasi dari bakteri Vibrio sp. yang bersimbiosis dengan spons laut Hyrtios altus. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa Trisindolina 5 terhadap ekspresi gen c-Myc dan cyclin D pada sel HeLa. Metode yang digunakan meliputi uji sitotoksisitas dengan metode MTT assay, dilanjutkan dengan uji ekspresi gen menggunakan teknik qRT-PCR. Hasil menunjukkan bahwa Trisindolina 5 mampu menurunkan viabilitas sel seiring peningkatan konsentrasi dengan sitotoksik lemah dimana nilai IC₅₀ sebesar 297,42 µg/mL. Selanjutnya, analisis ekspresi gen metode Livak mengindikasikan peningkatan ekspresi gen c-Myc tertinggi pada konsentrasi ½ IC₅₀ sebesar 20,11 kali lipat. Sementara gen cyclin D mengindikasikan penurunan tertinggi pada konsentrasi 4IC₅₀ dimana sebesar 0,014 kali lipat. Kesimpulan penelitian ini adalah Trisindolina 5 berpotensi sebagai agen penghambat proliferasi sel kanker melalui penurunan ekspresi cyclin D pada jalur siklus sel, namun belum cukup kuat untuk dijadikan agen antikanker langsung pada sel HeLa.
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Cervical cancer is a malignant tumor that threatens 10% to 40% of women's health where the main cause is infection from human papillomavirus (HPV). One of the genes that play a role in cancer development and proliferation is c-Myc which can induce cyclin D gene expression in the G1 to S phase transition of the cell cycle. Cancer treatments such as chemotherapy or radiotherapy still find subpopulations of HeLa cells that have the potential to produce tumors again. So it is necessary to develop specific cancer treatments by utilizing natural ingredients such as Trisindoline which is an indole derived compound isolated from Vibrio sp. bacteria that are symbiotic with the sea sponge Hyrtios altus. This study aims to determine the activity of Trisindoline 5 compound on c-Myc and cyclin D gene expression in HeLa cells. The method used includes cytotoxicity test with MTT assay method, followed by gene expression test using qRT-PCR technique. The results showed that Trisindoline 5 was able to reduce cell viability as the concentration increased with weakly active cytotoxicity where the IC₅₀ value was 297.42 µg/mL. Furthermore, Livak method gene expression analysis indicated the highest increase in c-Myc gene expression at a concentration of ½ IC₅₀ by 20.11 fold change. While the cyclin D gene indicated the highest decrease at a concentration of 4IC₅₀ by 0,014 fold change. The conclusion of this study is that Trisindolina 5 has the potential to be an agent inhibiting cancer cell proliferation by reducing cyclin D expression in the cell cycle pathway, but is not strong enough to be used as a direct anticancer agent in HeLa cells.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	c-Myc, cyclin D, HeLa, Kanker, Trisindolina 5.c-Myc, cyclin D, Cancer, HeLa, Trisindoline 5.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QL Zoology Q Science > QR Microbiology > QR 201.T84 Tumors. Cancer
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Marifa Nisa Azzahro
Date Deposited:	01 Aug 2025 06:33
Last Modified:	01 Aug 2025 06:33
URI:	http://repository.its.ac.id/id/eprint/125341

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