Optimasi Biodekolorisasi Metil Oranye Oleh Pseudomonas aeruginosa Yang Terimobilisasi Pada Matriks SA-PVA-Bentonit Dengan Response Surface Methodology

Halimah, Adedilla (2025) Optimasi Biodekolorisasi Metil Oranye Oleh Pseudomonas aeruginosa Yang Terimobilisasi Pada Matriks SA-PVA-Bentonit Dengan Response Surface Methodology. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Metil oranye (MO) merupakan jenis pewarna azo yang banyak digunakan di industri tekstil yang memiliki sifat toksik dan sulit terdegradasi secara alami. Pada penelitian ini dilakukan pengoptimalan proses biodekolorisasi MO menggunakan bakteri Pseudomonas aeruginosa yang terimobilisasi pada matriks kombinasi sodium alginat (SA), polivinil alkohol (PVA), dan bentonit menggunakan crosslinker kalsium klorida (CaCl2) 4% dan asam borat 5% (w/v) untuk meningkatkan stabilitas mekanik beads. Optimasi kondisi dilakukan menggunakan metode Response Surface Methodology (RSM) dengan desain Box-Behnken terhadap tiga variabel utama, yaitu pH, suhu, dan waktu inkubasi. Hasil menunjukkan bahwa kondisi optimum dicapai pada pH 9,3, suhu 30,1 °C, dan waktu inkubasi 1,1 hari dengan efisiensi biodekolorisasi sebesar 93,5%. Meskipun model menunjukkan nilai lack of fit yang signifikan, karakterisasi dengan SEM-EDX dan FTIR mengonfirmasi adanya perubahan struktur dan gugus fungsi pada permukaan beads setelah proses dekolorisasi, yang menunjukkan keberhasilan proses adsorpsi dan degradasi. Sistem ini juga menunjukkan kemampuan penggunaan ulang hingga tiga kali dengan efisiensi yang menurun bertahap. Dengan demikian, pendekatan biologis berbasis imobilisasi bakteri ini memiliki potensi sebagai metode pengolahan limbah tekstil yang ramah lingkungan.
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Methyl orange (MO) is a type of azo dye widely used in the textile industry, known for its toxic nature and resistance to natural degradation. In this study, the optimization of the MO biodecolorization process was carried out using Pseudomonas aeruginosa immobilized in a matrix composed of sodium alginate (SA), polyvinyl alcohol (PVA), and bentonite. The immobilization process employed calcium chloride (CaCl₂) 4% and boric acid 5% (w/v) as crosslinkers to enhance the mechanical stability of the beads. Optimization was conducted using Response Surface Methodology (RSM) with a Box-Behnken design involving three key variables: pH, temperature, and incubation time. The results showed that the optimal condition was achieved at pH 9.3, a temperature of 30.1 °C, and an incubation period of 1.1 days, resulting in a biodecolorization efficiency of 93.5%. Although the model exhibited a significant lack of fit, characterization using SEM-EDX and FTIR confirmed structural and functional group changes on the bead surface after the decolorization process, indicating successful adsorption and degradation. The system also demonstrated reusability up to three cycles with a gradual decrease in efficiency. Therefore, this biologically based approach using immobilized bacteria shows potential as an environmentally friendly method for treating textile wastewater containing azo dyes.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodecolorization, Pseudomonas aeruginosa, Methyl Orange, Immobilization, Response Surface Methodology
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Adedilla Halimah
Date Deposited:	05 Aug 2025 00:57
Last Modified:	05 Aug 2025 00:57
URI:	http://repository.its.ac.id/id/eprint/127210

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