Aktivitas Senyawa N'-E-(2-Bromobenzylidene) Benzohydrazide Dan N'-E-(2-Nitrobenzylidene) Benzohydrazide Terhadap Kadar Apoptosis Pada Cancer Stem Cells (Csc)

Azhaar, Nadia (2022) Aktivitas Senyawa N'-E-(2-Bromobenzylidene) Benzohydrazide Dan N'-E-(2-Nitrobenzylidene) Benzohydrazide Terhadap Kadar Apoptosis Pada Cancer Stem Cells (Csc). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pengobatan kanker menggunakan kemoterapi masih menyisakan subpopulasi Cancer Stem Cell (CSC). CSC mampu melakukan cell renewal, sel diferensiasi, stemness, mudah rekurensi, dan resisten terhadap terapi konvensional. Selain itu, CSC memiliki kemampuan untuk meningkatkan anti-apoptosis. Usaha pencarian obat antikanker terus dilakukan sampai saat ini. Senyawa hidrazon merupakan kelompok senyawa organik yang berasal dari reaksi antara aldehid atau keton dengan hidrazina. Hidrazon mengandung ikatan azometin -CH=NNH- yang mempunyai aktivitas antikanker, antitumor, antioksidan, antiinflamasi, dan antikonvulsan. Penelitian ini bertujuan untuk mengetahui aktivitas Senyawa N'-E-(2bromobenzylidene) benzohydrazide dan N'-E-(2-nitrobenzylidene) benzohydrazide terhadap kadar apoptosis pada Cancer Stem Cell (CSC). Senyawa yang digunakan adalah N'-E-(2bromobenzylidene) benzohydrazide dan N'-E-(2-nitrobenzylidene) benzohydrazide. Kontrol positif digunakan obat kanker komersil yakni Doxorubicin. Uji apoptosis digunakan metode flowcytometry. Data apoptosis kemudian dianalisis secara statistic menggunakan uji non parametrik yakni uji Kruskal-Wallis H pada IBM Statistic 26.0 version. Hasil yang didapat yaitu Senyawa N'-E-(2-bromobenzylidene) benzohydrazide memiliki persentase apoptosis tertinggi pada konsentrasi ½ IC50 yakni sebesar 2,27%. Selanjutnya, senyawa N'-E-(2nitrobenzylidene) benzohydrazide memiliki persentase apoptosis tertinggi pada konsentrasi 1 IC50 yakni sebesar 1,77%. Senyawa Doxorubicin memiliki persentase apoptosis yaitu sebesar 45,20% dan 54,30%. Kesimpulan dari penelitian ini yaitu Senyawa N'-E-(2-bromobenzylidene) benzohydrazide dan N'-E-(2-nitrobenzylidene) benzohydrazide mampu menginduksi apoptosis dengan kadar apoptosis masing-masing 2,27% dan 1,77%.
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Cancer treatment using chemotherapy still leaves a subpopulation of Cancer Stem Cells (CSC). CSC is capable of cell renewal, cell differentiation, stemness, easy recurrence, and resistance to conventional therapy. In addition, CSC has the ability to enhance anti-apoptosis. The search for anticancer drugs continues to this day. Hydrazone compounds are a group of organic compounds derived from the reaction between aldehydes or ketones with hydrazine. Hydrazone contains azomethin -CH=NNH- bonds which have anticancer, antitumor, antioxidant, anti-inflammatory, and anticonvulsant activities. This study aims to determine the activity of compounds N'-E-(2 bromobenzylidene) benzohydrazide and N'-E-(2 nitrobenzylidene) benzohydrazide on levels of apoptosis in Cancer Stem Cells (CSC). The compounds used were N'-E-(2-bromobenzylidene) benzohydrazide and N'-E-(2nitrobenzylidene) benzohydrazide. The positive control used a commercial cancer drug, namely Doxorubicin. Apoptosis test used flowcytometry method. The apoptotic data was then analyzed statistically using a non-parametric test, namely the Kruskal-Wallis H test on IBM Statistic 26.0 version. The results obtained are that the compound N'-E-(2-bromobenzylidene) benzohydrazide has the highest percentage of apoptosis at a concentration of IC50 which is 2.27%. Furthermore, the compound N'-E-(2-nitrobenzylidene) benzohydrazide had the highest percentage of apoptosis at a concentration of 1 IC50 which was 1.77%. Doxorubicin compounds have apoptotic percentages of 45.20% and 54.30%, respectively. The conclusion of this study is that the compounds N'-E-(2-bromobenzylidene) benzohydrazide and N'-E-(2nitrobenzylidene) benzohydrazide were able to induce apoptosis with apoptotic levels of 2.27% and
1.77%, respectively.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Apoptosis, Benzaldehida, Benzohidrasida, Cancer Stem Cell, Doxorubicin, Apoptosis, Benzaldehyde, Benzohydracide, Cancer Stem Cell, Doxorubicin.
Subjects:	Q Science > QH Biology Q Science > QH Biology > QH601 Cell membranes
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Eko Sulistiono
Date Deposited:	30 Sep 2025 08:32
Last Modified:	30 Sep 2025 08:32
URI:	http://repository.its.ac.id/id/eprint/128491

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