Respon Morfologi, Anatomi, Fisiologi Dan Molekuler Tanaman Porang (Amorphophallus muelleri Blume) Asal Bulbil Pada Cekaman Salinitas

Febriawan, Zulfan (2022) Respon Morfologi, Anatomi, Fisiologi Dan Molekuler Tanaman Porang (Amorphophallus muelleri Blume) Asal Bulbil Pada Cekaman Salinitas. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Porang (Amorphophallus muelleri Blume) merupakan tanaman umbi-umbian yang dikenal memiliki kandungan glukomanan yang tinggi. Glukomanan yang tinggi menyebabkan permintaan akan porang juga tinggi sehingga terjadi perluasan budidaya porang pada lahan sub-optimal salah satunya lahan salin. Namun, habitat alami porang adalah kawasan hutan yang umumnya dianggap baik secara fisik dan kimiawi. Lahan salin tersebut dapat menyebabkan cekaman salinitas yang mengakibatkan perubahan terhadap morfologi, anatomi, fisiologi dan molekuler tanaman, sehingga cekaman salinitas menjadi faktor pembatas pertumbuhan dan produktivitas tanaman. Oleh karena itu, penelitian ini bertujuan untuk mengetahui respon morfologi, anatomi, fisiologi, dan molekuler tanaman porang asal bulbil pada cekaman salinitas. Penelitian dilakukan dengan menggunakan rancangan acak lengkap (RAL). Pemberian cekaman salinitas dengan konsentrasi NaCl 0,50,100,150 dan 200 mM selama 14 hari. Parameter yang diukur yaitu aspek morfologi, anatomi, fisiologi dan molekuler. Data dianalisis menggunakan Anova One-Way kemudian dilanjutkan dengan uji Tukey. Respon morfologi menunjukkan adanya beda nyata pada parameter panjang akar, berat dan diameter umbi batang (100-200 mM), tinggi tanaman (150-200 mM), dan luas daun (50-200 mM) dibandingkan dengan kontrol. Respon anatomi tanaman porang menunjukkan adanya peningkatan persentase stomata menutup (100%) dan kerapatan stomata (4%-24%) pada konsentrasi 50-200 Mm. Respon fisiologi menunjukkan adanya beda nyata pada klorofil (100-200 mM) dan kandungan glukomanan (50-200 mM) serta penurunan pada kecepatan transpirasi dan Net Assimilation Rate. Respon molekuler ditandai dengan adanya peningkatan ekspresi relatif gen SuSy2 (1,5-1,55 kali) dan penurunan (0,91-1,2 kali). CSLA3 mengalami peningkatan (1,91-3 kali) dan penurunan 1,93 kali dibanding kontrol. Oleh karena itu, cekaman salinitas berpengaruh terhadap respon morfologi, anatomi, fisiologi dan molekuler tanaman porang.
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Porang (Amorphophallus muelleri Blume) is a tuber plant known to have high glucomannan content. High glucomannan causes the demand for porang is also high, so there is an expansion of porang cultivation on sub-optimal land, which is saline land. However, the natural habitat of the porang is a forest area, which is generally considered to be both physically and chemically. The saline land can cause salinity stress which results in changes to plant morphology, anatomy, physiology and molecular, so that salinity stress becomes a limiting factor for plant growth and productivity. Therefore, this study aimed to determine the morphological, anatomical, physiological, and molecular responses of porang plants from bulbil under salinity stress. The study was conducted using a completely randomized design (CRD). Treatments of salinity stress with concentrations of NaCl 0, 50,100,150 and 200 Mm for 14 days. Parameters measured were morphological, anatomical, physiological and molecular aspects. The data were analyzed using Anova One-Way and continued with the Tukey test. Morphological responses showed significant differences in the parameters of root length, weight and tuber diameter (100-200 mM), plant height (150-200 mM), and leaf area (50-200 mM) compared to controls. The anatomical response of porang plants showed an increase in the percentage of stomatal closure (100%) and stomatal density (4%-24%) at a concentration of 50-200 Mm. The physiological response showed a significant difference in chlorophyll (100-200 mM) and glucomannan content (50-200 mM), also decrease in transpiration rate and Net Assimilation Rate. The molecular response was characterized by an increase in the relative expression of the SuSy2 gene (1.5-1.55 fold) and a decrease (0.91-1.2 fold). CSLA3 increased (1.91-3 fold) and decreased 1.93 fold compared to control. Therefore, salinity stress affects the morphological, anatomical, physiological and molecular responses of porang plants.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	CSLA3, Pertumbuhan, Produktivitas, Salinitas, SuSy2,Growth, Productivity, Salinity
Subjects:	Q Science > QK Botany > QK710 Plant physiology
Divisions:	Faculty of Natural Science > Biology > 46101-(S2) Master Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	15 Dec 2025 01:26
Last Modified:	15 Dec 2025 01:26
URI:	http://repository.its.ac.id/id/eprint/128957

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