Dewi, Aulia An Nisaa (2022) Kelimpahan Relatif Gen Pendegradasi Hidrokarbon Pada Rhizosfer Tanaman Spesies Ternaturalisasi Tanah Tercemar Minyak Bumi Di Tambang Minyak Wonocolo, Bojonegoro, Jawa Timur. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Susunan rantai karbon pada minyak bumi yang kompleks mengakibatkan sulit untuk terurai didalam tanah sehingga mengakibatkan pencemaran pada tanah. Untuk mengatasi permasalahan tersebut, pada penelitian ini digunakan rhizoremediasi yang memanfaatkan asosisasi mutualistik tanaman dan mikrobia perakaran. Pemilihan spesies mikroorganisme yang menggunakan enzim-enzim spesifik dalam proses degradasi limbah minyak bumi ini sangat mempengaruhi proses bioremediasi sehingga diperlukan metode tertentu untuk eksplorasi gen-gen pengkode enzim degradasi minyak bumi. Tujuan penelitian ini adalah mengetahui perbedaan kelimpahan gen pendegradasi hidrokarbon (C230, PAH-RHD, nahAc, alkB, dan assA) pada rhizosfer tanaman kersen (Muntingia calabura) dan rumput gajah (Pennisetum purpureum) yang tumbuh di tanah tercemar tinggi minyak bumi dan tercemar rendah minyak bumi serta untuk mengetahui korelasinya terhadap sifat fisikokimia tanah dan kandungan TPH (Total Petroleum Hydrocarbon). Metode yang dilakukan yaitu pengambilan serta pengawetan sampel rhizosfer tanaman dan sampel tanah, ekstraksi DNA, optimasi PCR dan RT-PCR. Lalu dilakukan analisis kadar air dan kandungan TPH dengan metode uji gravimetri dan GC-MS. Analisis statistik dilakukan dengan T-test dan korelasi Spearman. Hasil dari penelitian ini diketahui bahwa tidak ada perbedaan yang signifikan antara kelimpahan gen pendegradasi hidrokarbon pada rhizosfer tanaman ternaturalisasi serta tidak ada korelasi antara kelimpahan gen pendegradasi hidrokarbon dengan fisikokimia tanah dan TPH.
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The complex arrangement of carbon chains in petroleum makes it difficult to decompose in the soil, resulting in soil pollution. To overcome this problem, rhizoremediation is used in this study which utilizes the mutualistic association of plants and root microbes. The selection of types of microorganisms that use certain enzymes in the degradation process of petroleum waste greatly affects the bioremediation process so that certain methods are needed to explore the genes encoding petroleum degradation enzymes. The purpose of this study was to determine differences in the abundance of hydrocarbon-degrading genes (C230, PAH-RHD, nahAc, alkB, and assA) in the rhizosphere of cherry (Muntingia calabura) and elephant grass (Pennisetum purpureum) growing on high-oil and low-pollution soils crude oil and determine its correlation with soil physicochemical properties and TPH (Total Petroleum Hydrocarbon) content. The methods used are the collection and preservation of plant rhizosphere samples and soil samples, DNA extraction, PCR optimization and RT-PCR. Then the water content and TPH levels were analyzed using the gravimetric and GC-MS test methods. Statistical analysis was performed by T-test and Spearman correlation. The results of this study showed that there was no significant difference between the abundance of hydrocarbon-degrading genes in naturalized plant rhizosphere and no correlation between the abundance of hydrocarbon-degrading genes with soil physicochemistry and TPH.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Minyak bumi, Muntingia calabura, Pencemaran tanah, Pennisetum purpureum, Rhizoremediasi, Muntingia calabura, Pennisetum purpureum, petroleum, rhizoremediation, soil pollution |
| Subjects: | Q Science > QH Biology Q Science > QK Botany > QK751 Plants--Effect of air pollution on |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Mr. Marsudiyana - |
| Date Deposited: | 17 Dec 2025 03:43 |
| Last Modified: | 17 Dec 2025 03:43 |
| URI: | http://repository.its.ac.id/id/eprint/129030 |
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