Dekolorisasi Melanin Menggunakan Ko-Katalis Laccase Dan Manganese Peroxidase Dari Jamur Pelapuk Putih Trametes Polyzona

Wahyuwati, Frida Ayu (2022) Dekolorisasi Melanin Menggunakan Ko-Katalis Laccase Dan Manganese Peroxidase Dari Jamur Pelapuk Putih Trametes Polyzona. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Melanin adalah pigmen pewarna utama pada kulit, rambut, dan mata, serta berfungsi sebagai fotoproteksi terhadap paparan sinar ultraviolet. Namun, peningkatan sintesis melanin dapat menggelapkan kulit dan menyebabkan kulit hiperpigmentasi. Cara alternatif untuk mencerahkan kulit adalah dengan menghilangkan pigmen melanin. Melanin dapat didekolorisasi oleh jamur pelapuk putih (white-rot fungi) yang menghasilkan satu atau lebih enzim ligninolitik ekstraseluler. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh variasi waktu reaksi dan unit enzim terhadap persentase dekolorisasi melanin serta mengetahui morfologi melanin sebelum dan setelah perlakuan enzimatik dengan analisis scanning electron microscope (SEM). Metode yang digunakan dalam penelitian ini yaitu produksi laccase dan manganese peroxidase, pengukuran aktivitas enzim, pemurnian parsial dan pemekatan enzim, uji dekolorisasi melanin dengan spektrofotometer, dilanjutkan dengan analisis SEM sebelum dan setelah perlakuan enzimatik. Kemudian, rancangan percobaan dalam penelitian ini dilakukan secara deskriptif kuantitatif dan kualitatif.
Hasil penelitian menunjukkan bahwa persentase dekolorisasi melanin tertinggi yaitu sebesar 71,24% selama reaksi 24 jam dengan kombinasi unit enzim 10 U laccase + 5,1 U manganese peroxidase. Ko-katalis laccase dan manganese peroxidase berpengaruh dalam mendekolorisasi melanin dengan hasil analisis SEM. Hasil SEM melanin setelah perlakuan enzimatik menunjukkan morfologi melanin sintetik terkoyak, permukaan kasar dan berpori, serta memiliki ukuran yang kecil seragam dibandingkan dengan sebelum perlakuan (kontrol) yang memiliki permukaan halus, bentuk bulat cekung, dan ukuran lebih besar.
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Melanin is the main coloring pigment in skin, hair, and eyes, and functions as a photoprotection against exposure to ultraviolet light. However, an increase in melanin synthesis can darken the skin and cause hyperpigmentation of the skin. An alternative way to lighten the skin is to remove the melanin pigment. Melanin can be decolorized by white-rot fungi which produce one or more extracellular ligninolytic enzymes. The purpose of this study was to determine the effect of variations in reaction time and enzyme units on the rate of melanin decolorization, and to determine the morphology of melanin before and after enzymatic treatment using scanning electron microscope (SEM) analysis. The methods that used in this study were the production of laccase and manganese peroxidase, measurement of enzyme activity, partial purification and concentration of the enzyme, melanin decolorization test with a spectrophotometer, followed by SEM analysis for observations before and after enzymatic treatment. Then, the experiment in this study had been carried out with quantitative and qualitative descriptive.
The results showed that the highest rate of decolorization of melanin was 71.24% during a 24-hour reaction with a combination of 10 U laccase + 5,1 U manganese peroxidase enzyme units. Co-catalyst laccase and manganese peroxidase had an effect on decolorizing melanin by SEM analysis. The results of SEM melanin after enzymatic treatment showed that the morphology of synthetic melanin was torn apart, the surface was rough and porous, and had a small uniform size compared to the pre-treatment (control) which had a smooth surface, a concave round shape, and a larger size.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Laccase, Manganese peroxidase, Melanin, SEM, Trametes polyzona, Laccase, Manganese Peroxidase, Melanin, SEM, Trametes polyzona
Subjects:	Q Science > QH Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	18 Dec 2025 10:43
Last Modified:	18 Dec 2025 10:43
URI:	http://repository.its.ac.id/id/eprint/129073

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