Modifikasi Enzimatik Hidrolase Dan Oksidoreduktase Pada Ekstraksi Rice Bran Protein (RBP) Sebagai Kandidat Drug Carrier

Shavieka, Imada Mella (2026) Modifikasi Enzimatik Hidrolase Dan Oksidoreduktase Pada Ekstraksi Rice Bran Protein (RBP) Sebagai Kandidat Drug Carrier. Masters thesis, Institut Teknologi Sepuluh Nopember.

Text 6005242006-Master_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (4MB) | Request a copy

Abstract

Rice bran kaya akan protein dan senyawa bioaktif, namun pemanfaatannya masih terbatas. Salah satu kendala utama adalah ikatan protein dalam matriks lignoselulosa yang kompleks, sehingga diperlukan metode ekstraksi dan modifikasi yang tepat untuk meningkatkan nilai fungsionalnya. Penelitian ini bertujuan mengetahui pengaruh pre-treatment selulase-lakase terhadap residu lignoselulosa pada efektifitas ekstraksi rice bran protein (RBP) dan modifikasi enzimatik hasil ekstrak RBP sebagai kandidat drug carrier. Kombinasi enzim oksidoreduktase (lakase) dan hidrolase (selulase) digunakan sebagai agen pre-treatment untuk perubahan struktur rice bran guna mempermudah pelepasan protein saat ekstraksi secara ultrasonikasi. Pre-treatment optimal terdapat pada perlakuan kombinasi lakase selulase pada suhu 60°C dengan total protein tertinggi yaitu sebesar 0,70 ± 0,19 mg/ml. Modifikasi gugus fungsi protein yang dihasilkan dilakukan dengan cara hidrolisis parsial menggunakan protease dengan peningkatan DLC hingga 79,44 ± 6,84% yang terjadi pada volume protease 75 μl. Analisis gugus fungsi protein menggunakan Fourier Transform Infrared Spectroscopy (FTIR) menghasilkan pergeseran gugus fungsi terutama gugus –OH, –NH, Amida I dan II ketika dibandingkan dengan kontrol (tanpa modifikasi enzimatik). Pengujian karakter struktur dan morfologi partikel RBP dilakukan dengan Scanning Electron Microscope (SEM) dengan RBP yang dimodifikasi enzimatik dihasilkan struktur yang terfragmentasi dengan distribusi partikel yang lebih longgar yang mendukung proses adsorpsi molekul kurkumin dengan matriks RBP. Performa drug carrier dibuktikan dengan meningkatnya bioavaibilitas secara signifikan dengan total kurkumin mencapai 166,7 ± 24,6 ng/ml pada formula Cur-RBPLS. Hasil penelitian ini menunjukkan bahwa kombinasi pre-treatment selulase–lakase dan modifikasi protease efektif dalam menghasilkan RBP dengan sifat struktural dan fungsional yang mendukung aplikasinya sebagai kandidat drug carrier.
========================================================================================================================
Rice bran is rich in protein and bioactive compounds, but its utilization is still limited. One of the main obstacles is the protein binding in the complex lignocellulose matrix, so that appropriate extraction and modification methods are needed to increase its functional value. This study aims to determine the effect of cellulase-laccase pretreatment on lignocellulose residues on the effectiveness of rice bran protein (RBP) extraction and enzymatic modification of the resulting RBP extract as a drug carrier candidate. A combination of oxidoreductase (laccase) and hydrolase (cellulase) enzymes was used as a pretreatment agent to change the structure of rice bran to facilitate protein release during ultrasonic extraction. Optimal pretreatment was found in the combination of laccase cellulase at a temperature of 60°C with the highest total protein of 0.70 ± 0.19 mg/ml. Modification of the resulting protein functional groups was carried out by partial hydrolysis using protease with an increase in DLC of up to 79.44 ± 6.84% which occurred at a protease volume of 75 μl. Analysis of protein functional groups using Fourier Transform Infrared Spectroscopy (FTIR) resulted in a shift in functional groups, especially the –OH, –NH, Amide I and II groups when compared to the control (without enzymatic modification). Testing of the structural and morphological characteristics of RBP particles was carried out using a Scanning Electron Microscope (SEM) with enzymatically modified RBP resulting in a fragmented structure with a looser particle distribution that supports the adsorption process of curcumin molecules with the RBP matrix. Drug carrier performance was proven by a significant increase in bioavailability with a total curcumin reaching 166.7 ± 24.6 ng/ml in the Cur-RBPLS formula. The results of this study indicate that the combination of cellulase-laccase pre-treatment and protease modification is effective in producing RBP with structural and functional properties that support its application as a drug carrier candidate.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Cellulase, Extraction, Laccase, Protease, Rice bran, Ekstraksi, Lakase, Protease, Rice bran, Selulase
Subjects:	Q Science > QD Chemistry > QD1 Oxidation-reduction reaction. Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD320 Cellulose. Hydrolysis Q Science > QD Chemistry > QD63 Extraction Q Science > QP Physiology > QP609.P78 Proteolytic enzymes. Q Science > QP Physiology > QP801.B69 Biopolymers
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Imada Mella Shavieka
Date Deposited:	21 Jan 2026 02:50
Last Modified:	21 Jan 2026 02:50
URI:	http://repository.its.ac.id/id/eprint/129912

Actions (login required)

View Item