Nuraini, Fayza Rahmalia Sabila (2026) Analisis Pengaruh Variasi Konsentrasi Montmorillonit(MMT) dan Glutaraldehyde pada Impregnasi Chitosan(CS)-Pelikel Selulosa Bakteri(BC) terhadap Sifat Mekanik dan Biologis Wound Dressing Hydrogel BC/CS/MMT-GA. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Luka kronis merupakan permasalahan medis yang banyak terjadi pada penderita diabetes dan pasien pasca operasi karena proses penyembuhan yang lambat serta risiko infeksi yang tinggi. Bacterial cellulose (BC) berpotensi sebagai material wound dressing modern karena struktur nanofiber berpori, biokompatibilitas tinggi, dan kemampuan mempertahankan kelembapan, namun masih memiliki keterbatasan pada sifat mekanik dan aktivitas antibakteri. Penelitian ini bertujuan meningkatkan performa hidrogel BC melalui metode impregnasi ex situ menggunakan larutan kitosan (CS) 2% dengan penambahan montmorillonite (MMT) pada variasi konsentrasi 0, 1, 3, 5, dan 7% serta penggunaan glutaraldehyde (GA) sebagai agen crosslinking pada konsentrasi 0,5%, 1%, dan 2%. Karakterisasi dilakukan menggunakan FTIR, XRD, SEM, gel fraction, swelling, water vapor transmission rate (WVTR), uji tekan, aktivitas antibakteri, dan uji viabilitas sel (MTT assay). Hasil uji tekan menunjukkan peningkatan kekuatan tekan dari 1,75 MPa pada sampel tanpa MMT menjadi 3,14 MPa pada sampel dengan MMT 7%. Rasio pembengkakan dan nilai gel fraction meningkat seiring penambahan MMT dan GA, dengan nilai gel fraction tertinggi sebesar 96,958% pada sampel MMT 7% dan GA 2%, yang mengindikasikan terbentuknya jaringan hidrogel yang lebih stabil. Sebaliknya, nilai WVTR menurun akibat meningkatnya kerapatan struktur, dengan nilai tertinggi yang masih memenuhi standar wound dressing ideal sebesar 2418,237 g/(m²·day) pada sampel tanpa MMT dan 2276,564 g/(m²·day) pada sampel MMT 1%. Uji antibakteri menunjukkan terbentuknya zona hambat optimum sebesar 12,5 mm terhadap Staphylococcus aureus pada sampel MMT 3% dan 7% dengan GA 1%, serta 8 mm terhadap Escherichia coli pada sampel MMT 7% dengan GA 1%. Analisis SEM menunjukkan pengisian pori dan struktur yang semakin padat, sementara FTIR dan XRD mengonfirmasi interaksi fisik antar komponen tanpa perubahan struktur kristalin utama BC. Beberapa sampel, yaitu BC/CS-GA 0,5%, BC/CS/MMT1-GA 0,5%, BC/CS-GA 1%, BC/CS/MMT1-GA 1%, dan BC/CS-GA 2%, memenuhi karakteristik wound dressing ideal.
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Chronic wounds are a common medical problem in diabetics and post-operative patients due to slow healing processes and high risk of infection. Bacterial cellulose (BC) has potential as a modern wound dressing material due to its porous nanofiber structure, high biocompatibility, and moisture retention capabilities, but it still has limitations in terms of mechanical properties and antibacterial activity. This study aims to improve the performance of BC hydrogel through the ex situ impregnation method using a 2% chitosan (CS) solution with the addition of montmorillonite (MMT) at varying concentrations of 0, 1, 3, 5, and 7% and the use of glutaraldehyde (GA) as a crosslinking agent at concentrations of 0.5%, 1%, and 2%. Characterization was performed using FTIR, XRD, SEM, gel fraction, swelling, water vapor transmission rate (WVTR), compression test, antibacterial activity, and cell viability test (MTT assay). The compression test results showed an increase in compressive strength from 1.75 MPa in samples without MMT to 3.14 MPa in samples with 7% MMT. The swelling ratio and gel fraction value increased with the addition of MMT and GA, with the highest gel fraction value of 96.958% in the 7% MMT and 2% GA sample, indicating the formation of a more stable hydrogel network. Conversely, the WVTR value decreased due to the increased density of the structure, with the highest value still meeting the ideal wound dressing standard of 2418.237 g/(m²·day) in the sample without MMT and 2276.564 g/(m²·day) in the 1% MMT sample. Antibacterial testing showed the formation of an optimal inhibition zone of 12.5 mm against Staphylococcus aureus in the 3% and 7% MMT samples with 1% GA, and 8 mm against Escherichia coli in the 7% MMT sample with 1% GA. SEM analysis showed pore filling and increasingly dense structure, while FTIR and XRD confirmed physical interaction between components without changes in the main crystalline structure of BC. Several samples, namely BC/CS-GA 0.5%, BC/CS/MMT1-GA 0.5%, BC/CS-GA 1%, BC/CS/MMT1-GA 1%, and BC/CS-GA 2%, met the characteristics of an ideal wound dressing.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | selulosa bakteri, montmorillonite, glutaraldehyde, impregnasi, wound dressing, hidrogel. bacterial cellulose, montmorillonite, Glutaraldehyde, impregnation, wound dressing, hydrogel. |
| Subjects: | Q Science Q Science > QC Physics > QC173.4.C63 Composite materials Q Science > QH Biology > QH541.15.T68 Toxicity testing Q Science > QP Physiology > QP801.P64 Polymers--Biodegradation |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Fayza Rahmalia Sabila Nuraini |
| Date Deposited: | 22 Jan 2026 08:18 |
| Last Modified: | 22 Jan 2026 08:18 |
| URI: | http://repository.its.ac.id/id/eprint/130072 |
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