Pengaruh Kombinasi Amilase Dan Cellobiose Dehydrogenase (CDH) Dalam Proses Penghilangan Kanji (Desizing) Tekstil

Arifin, Auliyya Intan Mellaniy (2026) Pengaruh Kombinasi Amilase Dan Cellobiose Dehydrogenase (CDH) Dalam Proses Penghilangan Kanji (Desizing) Tekstil. Masters thesis, Institut Teknologi Sepuluh Nopember.

Text 6005242003-Master_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (4MB) | Request a copy

Abstract

Kanji (pati) pada proses sizing tekstil dapat menyebabkan kain menjadi kusam serta menurunkan daya serap air dan zat warna, sehingga perlu dihilangkan melalui proses desizing. Proses desizing umumnya menggunakan bahan kimia yang berpotensi merusak kelembutan kain dan mencemari lingkungan, sehingga pengembangan metode berbasis enzim menjadi alternatif lebih ramah lingkungan. Amilase telah banyak digunakan sebagai agen desizing karena kemampuannya mendegradasi pati, namun penerapan kombinasi enzim dalam satu rangkaian proses industri untuk meningkatkan efektivitas desizing sekaligus pemutihan tekstil masih terbatas. Kombinasi amilase dan cellobiose dehydrogenase (CDH) berpotensi bekerja secara sinergis karena amilase berperan dalam hidrolisis pati, sedangkan CDH berkontribusi melalui oksidasi pengotor non-pati, seperti lignin, sehingga meningkatkan kecerahan kain. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh kombinasi amilase dan cellobiose dehydrogenase (CDH), suhu, serta waktu inkubasi terhadap efektivitas proses desizing tekstil dan struktur permukaan kain. Amilase diproduksi oleh Bacillus subtilis menggunakan media basal dan dikarakterisasi melalui pemurnian, penentuan kadar protein, serta titik isoelektrik, sedangkan CDH diproduksi oleh Trametes versicolor. Proses desizing dilakukan dengan variasi kombinasi enzim, suhu, dan waktu inkubasi, diikuti pencucian dan pengeringan kain. Efektivitas desizing dianalisis berdasarkan berat sisa kanji, kadar gula pereduksi menggunakan metode DNS, struktur serat kain, dan warna kain. Hasil penelitian menunjukkan kombinasi amilase dan CDH meningkatkan efektivitas desizing dibandingkan perlakuan tanpa kombinasi enzim. Perlakuan pada suhu 60°C menghasilkan penghilangan pati paling efektif, ditandai dengan hasil persentase penurunan berat kanji sebesar 99,69%, kadar gula pereduksi tertinggi sebesar 24,47 mg/ml, serta sisa pati (skala TEGEWA) 9. Namun, peningkatan kecerahan warna dan kualitas struktur permukaan serat paling optimal diperoleh pada perlakuan kombinasi enzim suhu 50°C dengan waktu inkubasi 48 jam, ditunjukkan oleh nilai intensitas RGB (keputihan warna) yang lebih tinggi (R: 228,8 G: 223,01 B: 221) serta permukaan serat yang lebih bersih rapat, dan homogen.
================================================================================================================================
Starch in the textile starching process can cause fabrics to become dull and reduce their water and dye absorption, so it needs to be removed through a desizing process. Conventional desizing processes generally use chemicals that have the potential to damage the softness of fabrics and pollute the environment, so the development of enzyme-based methods is a more environmentally friendly alternative. Amylase has been widely used as a desizing agent due to its ability to degrade starch, but the application of enzyme combinations in a series of industrial processes to increase the effectiveness of desizing and bleaching textiles is still limited. The combination of amylase and cellobiose dehydrogenase (CDH) has the potential to work synergistically beacause amylase plays a role in starch hydrolysis, while CDH contributes through the oxidation of non-starch impurities, such as lignin, thereby increasing fabric brightness. The purpose of this study was to evaluate the effect of combination of amylase and cellobiose dehydrogenase (CDH), temperature, and incubation time on the effectiveness of the textile desizing process and the fabric surface structure. Amylase is produced by Bacillus subtilis using basal media and is characterized through purification, determination of protein content, and isoelestric point, while CDH is produced by Trametes versicolor. The desizing process was carried out with various combinations of enzymes, temperatures, and incubation times, followed by washing and drying the fabric. The effectiveness of desizing was analyzed based on the residual starch weight, reducing sugar content using the DNS method, fabric fiber structure, and fabric color. The results showed that the combination of amylase and CDH increased the effectiveness of desizing compared to the treatment without enzyme combination. Treatment at a temperature of 60°C resulted in the most effective starch removal, characterized by a % starch weight reduction of 99,69%, and the highest reducing sugar content of 24,47 mg/ml, as well as a TEGEWA scale of 9. However, the most optimal increase in color brightness and fiber surface structure quality was obtined in the enzyme combination at a temperature of 50°C with an incubation time of 48 hours, indicated by higher RGB intensity values (R: 228,8 G: 223,01 B: 221) as well as a cleaner, denser, and more homogeneous fiber surface.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Amilase, CDH, Desizing, SEM, Tekstil, Amylase, CDH, Desizing, SEM, Tekstil
Subjects:	Q Science Q Science > QH Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Auliyya Intan Mellaniy Arifin
Date Deposited:	29 Jan 2026 04:07
Last Modified:	29 Jan 2026 04:07
URI:	http://repository.its.ac.id/id/eprint/130740

Actions (login required)

View Item