Paper Analytical Device Terimobilisasi Bathopenanthroline Mikrokristal Untuk Deteksi Dopamin

Afkar, Daffa Dira Izzi Al (2026) Paper Analytical Device Terimobilisasi Bathopenanthroline Mikrokristal Untuk Deteksi Dopamin. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Penyakit Parkinson merupakan gangguan neurodegeneratif global yang banyak diderita manusia. Dopamin bisa dijadikan salah satu bioindikator untuk mendeteksi adanya penyakit parkinson. Akan tetapi, metode analisis dopamin sebagai bioindikator saat ini umumnya mahal, memerlukan instrumen canggih, dan waktu analisis yang lama. Oleh karena itu, dalam penelitian ini dikembangkan Microfluidic Paper-based Analytical Device yang terintegrasi dengan bathophenanthroline mikrokristal (BATH MCs) disintesis sebagai sensor kolorimetri yang ekonomis, praktis, dan sensitif untuk deteksi dopamin. BATH MCs menggunakan metode reprecipitasi dan dikarakterisasi menggunakan Field Emission Scanning Electron Microscopy (FESEM) serta Particle Size Analyzer (PSA), yang menunjukkan distribusi ukuran partikel rata-rata 715,7 nm. Prinsip deteksi didasarkan pada reaksi oksidasi-reduksi di mana dopamin mereduksi Fe³⁺ menjadi Fe²⁺, yang kemudian membentuk kompleks berwarna merah dengan BATH MCs pada permukaan µPAD. Perubahan intensitas warna dianalisis secara digital menggunakan perangkat lunak ImageJ pada photo light box. Pada penelitian ini, kondisi optimum ditentukan dengan menguji pengaruh FeCl3, menguji waktu pengeringan dari 0-40 menit, rentang konsentrasi pengujian dopamin sehingga didapatkan kondisi optimum deteksi diperoleh dengan penggunaan FeCl₃ sebagai agen pengoksidasi dan waktu pengeringan selama 10 menit. Kurva kalibrasi menunjukkan linearitas yang baik (R2 = 0,9838) pada rentang konsentrasi 0–4 mM. Batas deteksi (LOD) dan batas kuantifikasi (LOQ) yang diperoleh masing-masing sebesar 1,34 mM dan 4,06 mM. Sensor ini juga menunjukkan selektivitas yang tinggi terhadap dopamin dibandingkan dengan berbagai senyawa interferen seperti glukosa, urea, dan ion logam lainnya. Penelitian ini membuktikan potensi µPAD berbasis BATH MCs sebagai alat diagnostik alternatif yang murah dan portabel untuk deteksi penyakit neurodegeneratif.
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Parkinson’s disease is a global neurodegenerative disorder that affects a large number of people worldwide. Dopamine can be used as a biomarker for the detection of Parkinson’s disease; however, current analytical methods for dopamine determination are generally expensive, require sophisticated instrumentation, and involve long analysis times. Therefore, this study developed a microfluidic paper-based analytical device (µPAD) integrated with bathophenanthroline microcrystals (BATH MCs) as an economical, practical, and sensitive colorimetric sensor for dopamine detection. Bathophenanthroline microcrystals were synthesized using a reprecipitation method and characterized by field emission scanning electron microscopy (FESEM) and particle size analysis (PSA), revealing an average particle size distribution of 715.7 nm. The detection principle is based on an oxidation–reduction reaction in which dopamine reduces Fe³⁺ to Fe²⁺, followed by the formation of a red-colored complex with BATH MCs on the µPAD surface. Changes in color intensity were digitally analyzed using ImageJ software in a photo light box system. The optimum detection conditions were determined by evaluating the effect of FeCl₃ concentration, drying time (0–40 min), and dopamine concentration range, and the optimal condition was achieved using FeCl₃ as the oxidizing agent with a drying time of 10 min. The calibration curve exhibited good linearity (R² = 0.9838) over a concentration range of 0–4 mM, with limits of detection (LOD) and quantification (LOQ) of 1.34 mM and 4.06 mM, respectively. The developed sensor also demonstrated high selectivity toward dopamine compared to various interfering substances, including glucose, urea, and metal ions. These results demonstrate the potential of the BATH MCs-based µPAD as a low-cost and portable alternative diagnostic tool for the detection of neurodegenerative disease.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Dopamin, Paper-based Analytical Device, Bathophenanthroline, Mikrokristal, Kolorimetri.
Subjects:	Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Analytical Science and Chemical Instrumentation > 47203-(S1) Undergraduate Thesis
Depositing User:	Daffa Dira Izzi Al Afkar
Date Deposited:	03 Feb 2026 07:54
Last Modified:	03 Feb 2026 07:55
URI:	http://repository.its.ac.id/id/eprint/131817

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