Nabilah, Badzlin (2022) Biodekolorisasi Metilen Biru Oleh Kultur Campuran Jamur Filamen Trichoderma Viride Dan Bakteri Ralstonia Pickettii Yang Terimobilisasi Dalam Matriks Natrium Alginat – Polivinil Alkohol-Bentonit. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Metilen biru (MB) merupakan salah satu pewarna sintetis yang digunakan dalam industri tekstil dan industri kertas, dimana pembuangannya dapat mencemari perairan. Pada penelitian ini dilakukan proses imobilisasi kultur campuran jamur T. viride dan bakteri R. pickettii ke dalam matriks Sodium alginate-Polivinil Alkohol-Bentonit (SA-PVA-Bentonit). Proses imobilisasi sendiri dilakukan dengan metode entrapment, dimana sel bakteri dan sel jamur dihomogenkan dengan matriks SA-PVA-Bentonit. Beads yang sudah terbentuk kemudian digunakan dalam proses biodekolorisasi MB. Berdasarkan hasil screening beads dapat diketahui komposisi SA-PVA-Bentonit 1/4/1% (%b/v) menghasilkan beads yang berbentuk bulat dan tidak tailing. Matriks dengan komposisi tersebut selanjutnya digunakan untuk proses imobilisasi kultur campuran T. viride dan R. pickettii. Pada saat proses biodekolorisasi MB, dapat diketahui kultur campuran terimobilisasi menunjukkan persentase MB removal tertinggi, yakni 97,88%. Proses biodekolorisasi tersebut menghasilkan 3 metabolit produk, yakni Azure A, Azure C, dan Thionine. Optimasi biodekolorisasi MB dengan metode RSM dilakukan untuk mengetahui kondisi optimum yang diperlukan beads dalam mendekolorisasi MB. Berdasarkan data hasil optimasi dapat diketahui bahwa kondisi suhu inkubasi 40 °C, konsentrasi MB mula – mula 50 mg/L, dan waktu inkubasi 72 jam menghasilkan persentase MB removal tertinggi, yakni sebesar 99,57%. Kondisi optimum tersebut digunakan dalam uji reusabilitas beads dan diperoleh hasil beads dapat digunakan hingga 3 kali siklus biodekolorisasi.
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Methylene blue (MB) is one of the synthetic dyes used in the textile and paper industries which its disposal can pollute the waters. In this study, mixed culture of T. viride and R. pickettii was immobilized into a Sodium Alginate-Polyvinyl Alcohol-Bentonite (SA-PVA-Bentonite) matrix. The immobilization process was carried out by the entrapment method, where the bacterium and the fungus cells were homogenized with the SA-PVA-Bentonite matrix. The beads that had been formed then used in the MB biodecolorization process. Based on the results of the beads screening test, it showed that the composition of SA-PVA-Bentonite 1/4/1% (%w/v) produced spherical shaped beads. Thus, the matrix with this composition was used for the immobilization process of mixed cultures of T. viride and R. pickettii. During the MB biodecolorization process, it could be seen that the immobilized mixed culture showed the highest MB removal percentage, which was 97.88%. The biodecolorization process itself produced 3 product metabolites Azure A, Azure C, and Thionine. Optimization of MB biodecolorization with RSM method was carried out to determine the optimum conditions required for beads to decolorize MB. The optimization result showed that the highest MB removal was achieved 99.57% when the incubation temperature was 40 °C, initial concentration was 50 mg/L, and the incubation period was 72 hours. These conditions were used in the beads reusability analysis and the results showed that the beads could be used up to 3 cycles of biodecolorization.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | RTKi 547.135 Nab b-1 2022 |
| Uncontrolled Keywords: | Biodekolorisasi; Metilen biru; Imobilisasi; Jamur filamen; Ralstonia pickettii. Biodecolorization; Methylene blue; Immobilization; Filamentous fungi; Ralstonia pickettii |
| Subjects: | Q Science > QP Physiology > QP517.B5 Biodegradation. |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis |
| Depositing User: | Mr. Marsudiyana - |
| Date Deposited: | 30 Apr 2026 08:29 |
| Last Modified: | 30 Apr 2026 08:29 |
| URI: | http://repository.its.ac.id/id/eprint/132947 |
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