Maria, Virda Putri (2022) Biodekolorisasi Metil Oranye Oleh Ralstonia pickettii Yang Terimobilisasi Pada Matriks Sa-Pva-Bentonit. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Salah satu pewarna yang banyak digunakan dalam industri tekstil adalah metil oranye (MO). Limbah MO juga terkenal sebagai pewarna karsinogen dan sulit terdegradasi sehingga dapat menyebabkan terganggunya ekosistem lingkungan. Biodekolorisasi menggunakan mikrooganisme dapat digunakan sebagai solusi dalam mengatasi limbah MO. Biodekolorisasi dibedakan menjadi dua metode yaitu metode sel bebas (free cell) dan metode imobilisasi sel. Namun, metode free cell lebih sensitif terhadap perubahan suhu dibandingkan imobilisasi sel. Bakteri Ralstonia pickettii berperan penting dalam dekolorisasi pewarna karena dapat memanfaatkan hidrokarbon aromatik pada MO sebagai sumber karbon dan energi. Oleh karena itu, penelitian ini menggunakan bakteri R. pickettii yang terimobilisasi pada matriks SA-PVA- bentonit dalam dekolorisasi 50 mg/L MO. Karakterisasi beads sebelum dan sesudah dekolorisasi MO telah dilakukan dengan FTIR dan SEM untuk mengidentifikasi gugus fungsi dan melihat morfologi serta topografi dari beads. Pada penelitian ini, didapatkan hasil dekolorisasi MO selama 10 hari oleh beads kosong, beads mati, dan beads hidup sebesar 33,48%, 33,74%, dan 66,63%. Beads imobilisasi ini dapat digunakan hingga dua kali siklus dalam dekolorisasi MO. Berbeda dengan hasil dekolorisasi MO oleh free cell hidup dan mati, hanya didapatkan masing-masing sebesar 27,45% dan 9,59%. Dari hasil tersebut dapat disimpulkan bahwa bakteri R. pickettii yang terimobilisasi pada matriks SA-PVA-bentonit lebih efektif dalam dekolorisasi MO dibandingkan bakteri free cell
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One of the dyes widely used in the textile industry is methyl orange (MO). MO waste is also known as a carcinogenic dye and is difficult to degrade so it can cause disruption of environmental ecosystems. Biodecolorization using microorganisms can be used as a solution in dealing with MO waste. Biodecolorization is divided into two methods, namely the free cell method and the cell immobilization method. However, the free cell method is more sensitive to temperature changes than cell immobilization. Ralstonia pickettii plays an important role in the decolorization of dyes because it can utilize the aromatics in MO as a source of carbon and energy. Therefore, this study used R. pickettii bacteria immobilized in SA-PVA-bentonite matrix in 50 mg/L MO decolorization. Characterization of the bead before and after dye decolorization of MO using FTIR and SEM to identify functional groups and observe the morphology and topography of the beads. In this study, the results of the decolorization of MO for 10 days by empty beads, dead beads, and live beads were 33,48%, 33,74%, and 66.63%, respectively. These immobilized beads can be used for up to two cycles in MO decolorization. In contrast, the results of MO decolorization by live and dead free cells were only 27,45% and 9,59%, respectively. From these results, it concluded that R. pickettii bacteria immobilized in SA-PVA-bentonite matrix were more effective in decolorizing MO compared to bacteria-free cells.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | RSKi 547.135 Mar b-1 2022 |
| Uncontrolled Keywords: | Biodekolorisasi, Metil Oranye, Ralstonia pickettii, Imobilisasi, Bentonit. Biodecolorization, Methyl orange, Ralstonia pickettii, Immobilization, Bentonite. |
| Subjects: | Q Science > QD Chemistry > QD75.2 Chemistry, Analytic |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Mr. Marsudiyana - |
| Date Deposited: | 07 May 2026 08:56 |
| Last Modified: | 08 May 2026 01:37 |
| URI: | http://repository.its.ac.id/id/eprint/132987 |
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