Biodekolorisasi Metil Oranye Oleh Bacillus subtilis Yang Terimobilisasi Pada Matriks SA-PVA.

Rohma, Nova Ainur (2022) Biodekolorisasi Metil Oranye Oleh Bacillus subtilis Yang Terimobilisasi Pada Matriks SA-PVA. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Limbah dari zat warna metil oranye (MO) bersifat karsinogenik, mutagenik, dan mudah larut air sehingga dibutuhkan upaya dalam mengatasinya. Penelitian sebelumnya menyatakan bahwa Bacillus subtilis dapat mendekolorisasi zat warna MO, namun penggunaan sel bebas dari bakteri memiliki kelemahan karena bakteri tidak mampu bertahan dalam kondisi yang toksik dan tidak dapat digunakan kembali. Salah satu upaya yang dapat mengatasi masalah tersebut adalah dengan imobilisasi sel. Tujuan penelitian ini adalah mempelajari biodekolorisasi pewarna MO oleh B. subtilis yang terimobilisasi dalam matriks sodium alginate-polyvinyl alcohol (SA-PVA). Bakteri B. subtilis diimobilisasi menggunakan metode entrapment dengan komposisi matriks SA-PVA 2:4 (% b/v). Proses dekolorisasi MO dilakukan dengan metode sel bebas bakteri dan imobilisasi dengan variasi perlakuan bakteri hidup dan mati. Biodekolorisasi MO dilakukan pada media air dengan konsentrasi 50 ppm dalam keadaan statis dan diamati selama 10 hari inkubasi. Sel bebas bakteri hidup (FC-BSH) dapat mendekolorisasi MO sebesar 34,61%, sedangkan sel bebas bakteri mati (FC-BSM) hanya sebesar 7,44%. Kinerja biodekolorisasi oleh imobilisasi bakteri menunjukkan beads imobilisasi bakteri hidup (SA-PVA-BSH) memiliki keunggulan dalam mendegradasi MO sebesar 96,9%, sedangkan beads imobilisasi bakteri mati (SA-PVA-BSM) dan beads tanpa bakteri (SA-PVA-K) masing-masing hanya sebesar 26,43% dan 24,11%. Keberhasilan imobilisasi juga didukung oleh data spektra FTIR, mikrograf SEM, dan spektra EDX. Beads imobilisasi memiliki stabilitas dan reusability yang baik setelah tiga siklus dekolorisasi MO. Hasil ini menunjukkan bahwa B. subtilis yang terimobilisasi dalam matriks SA-PVA dapat digunakan sebagai kandidat baru untuk dekolorisasi MO yang efektif.
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Waste from methyl orange (MO) dyes is carcinogenic, mutagenic, and easily soluble in water, so efforts are needed to overcome it. Previous research stated that Bacillus subtilis can decolorize MO dyes, but the use of cells free from bacteria has a weakness because bacteria cannot survive in toxic conditions and cannot be reused. One of the efforts that can be solved this problem is the use of cell immobilization. This research aimed to study the biodecolorization of MO dye by B. subtilis immobilized in SA-PVA matrix. Bacteria were immobilized using the entrapment method with a matrix composition of Sodium alginate-polyvinyl alcohol (SA-PVA) with ratio 2:4 (% w/v). The decolorization process was carried out by the bacteria-free cell and immobilization methods with various treatments of live and dead bacteria. MO biodecolorization was carried out in aqueous media with a concentration of 50 ppm under static conditions and observed for 10 days of incubation. The free cell of live bacteria (FC-BSH) decolorized MO by 34.61%, while cells free of dead bacteria (FC-BSM) was only 7.44%. The biodecolorization performance by immobilized bacteria showed that immobilized living bacteria (SA-PVA-BSH) beads had advantages in degrading MO by 96.9%, while immobilized dead bacteria (SA-PVA-BSM), and immobilized without bacteria (SA-PVA-K) beads was only 26.43% and 24.11%, respectively. The success of immobilization was supported by FTIR spectra, SEM micrographs, and EDX spectra. The immobilized beads had good stability and reusability after three cycles of MO decolorization. These results indicated that B. subtilis immobilized in the SA-PVA matrix can be used as a new candidate for effective MO decolorization.

Item Type:	Thesis (Other)
Additional Information:	RSKi 547.135 Roh b-1 2022
Uncontrolled Keywords:	Bacillus subtilis, Imobilisasi, Sodium alginate, Polivinil alkohol, Metil oranye. Bacillus subtilis, Immobilization, sodium alginate, polyvinyl alcohol, methyl orange.
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	06 May 2026 02:31
Last Modified:	06 May 2026 02:31
URI:	http://repository.its.ac.id/id/eprint/132998

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