Fragandi, Hanggi (2022) Biodekolorisasi Metil Oranye Oleh Bakteri Bacillus subtilis Terimobilisasi Pada Matriks Sa/Pva/Arang Aktif. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Metil oranye (MO), salah satu pewarna tekstil dari golongan azo yang digunakan pada industri tekstil, memiliki dampak negatif bagi kehidupan manusia dan lingkungan. Banyak upaya telah dilakukan untuk menemukan metode yang paling efektif dalam pengurangan pencemaran MO. Bakteri Bacillus Subtilis telah diidentifikasi sebagai agen biodekolorisasi MO, namun efisiensinya masih rendah.Oleh karena itu perlu dicari metode lain untuk mendapatkan efisiensi yang lebih tinggi. Dalam penelitian ini, kemampuan bakteri B. Subtilis untuk mendekolorisasi MO dengan metode imobilisasi menggunakan matriks Sodium Alginat/Polivinil Alkohol/Arang Aktif (SA/PVA/AA) telah diteliti. B. subtilis ditambahkan dalam matriks SA/PVA/AA dengan komposisi 2%:4%:1% (b/v). Variasi beads yang digunakan yaitu SA/PVA/AA, SA/PVA/AA/Bakteri Mati, dan SA/PVA/AA/Bakteri Hidup. Proses biodekolorisasi dilakukan dengan dua metode menggunakan sel bebas bakteri dan bakteri terimobilisasi pada larutan Mo konsentrasi 50 mg/L sebanyak 100 ml. Hasil penelitian diperoleh persentase kemampuan mendekolorisasi menggunakan metode imobilisasi lebih besar pada variasi beads SA/PVA/AA, SA/PVA/AA/Bakteri Mati, dan SA/PVA/AA/Bakteri Hidup secara berturut-turut 37,4%; 38,19%; 60,19%. Penggunaan sel bebas bakteri mati diperoleh persen dekolorisasi 11,7% dan bakteri hidup 21,27% dengan masa inkubasi selama 10 hari dalam keadaan statis pada suhu 37 °C. Reuseabilitas beads SA/PVA/AA/Bakteri Hidup dapat digunakan kembali selama dua siklus. Keberhasilan imobilisasi B. subtilis mengunakan matriks SA/PVA/AA dapat dibuktikan dengan spektra FTIR dan visualisasi morfologi SEM-EDX. Hasil ini menunjukkan bahwa B. subtilis terimobilisasi matriks SA/PVA/Arang Aktif dapat digunakan sebagai metode alternatif dalam mendekolorisasi MO dan mengurangi permasalahan limbah pewarna tekstil.
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Methyl orange (MO), one of the textile dyes of the azo group used in the textile industry, has a negative impact on human life and the environment. Many attempts have been made to find the most effective method of MO pollution reduction. The bacterium Bacillus subtilis has been identified as a MO biodecolorizing agent. However, its efficiency is still low. Therefore, it is necessary to look for other methods to obtain higher efficiencies. In this study, the ability of B. subtilis bacteria to decolorize MO by an immobilization method using the Sodium Alginate/Polyvinyl Alcohol/Activated Charcoal (SA/PVA/AA) matrix has been studied. B. subtilis is added to the SA/PVA/AA matrix with a composition of 2%:4%:1% (w/v). The varieties of beads used are SA/PVA/AA, SA/PVA/AA/Dead Bacteria, and SA/PVA/AA/Live Bacteria. The biodecolorization process is carried out by two methods using bacteria-free cells and immobilized bacteria in a MO solution with a concentration of 50 mg/L per 100 mL. The results of the study obtained a greater percentage of decolorization ability using immobilization methods in the variations in beads SA/PVA/AA, SA/PVA/AA/Dead Bacteria, and SA/PVA/AA/Live Bacteria, respectively, 37,4%; 38,19%; and 60,19%. The use of dead bacteria-free cells obtained a 11,7% decolorization percentage and 21,27% live bacteria with an incubation period of 10 days in a static state at a temperature of 37 °C. Reusability of beads SA/PVA/AA/Live Bacteria can be reused over two cycles. The success of the immobilization of B. subtilis using the SA/PVA/AA matrix can be proved by FTIR spectra and SEM-EDX morphological visualization. These results suggest that B. subtilis immobilized SA/PVA/Activated Carbon matrix can be used as an alternative method for decolorizing MO and reducing the problem of textile dye waste.
| Item Type: | Thesis (Other) |
|---|---|
| Additional Information: | RSKi 547.135 Fra b-1 2022 |
| Uncontrolled Keywords: | Biodekolorisasi, Metil Oranye, Bacillus Subtilis, Imobilisasi, Arang Aktif. Biodecolorization, Methyl Orange, Bacillus Subtilis, Immobilization, Carbon Active. |
| Subjects: | Q Science > QP Physiology > QP517.B5 Biodegradation. |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Mr. Marsudiyana - |
| Date Deposited: | 07 May 2026 01:19 |
| Last Modified: | 08 May 2026 01:39 |
| URI: | http://repository.its.ac.id/id/eprint/133028 |
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