Biodekolorisasi Pewarna Metil Oranye Oleh Pseudomonas aeruginosa Yang Termobilisasi Dalam Matriks SA-PVA-BENTONIT

Misdar, Misdar (2022) Biodekolorisasi Pewarna Metil Oranye Oleh Pseudomonas aeruginosa Yang Termobilisasi Dalam Matriks SA-PVA-BENTONIT. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pewarna metil oranye (MO) merupakan pewarna sintetis yang banyak digunakan dalam industri tekstil. Limbah MO dapat bersifat beracun, mutagenik, dan karsinogenik, jika dibuang langsung ke lingkungan tanpa suatu penanganan yang tepat. Bioremediasi atau biodekolorisasi menggunakan kultur bakteri dapat menjadi solusi untuk mengurangi limbah MO. Akan tetapi, penggunaan bakteri dengan tehnik sel bebas memiliki beberapa kekurangan, salah satunya rentan terhadap kondisi perubahan atau tekanan lingkungan, seperti keterbatasan jumlah nutrisi. Tujuan pada penelitian ini adalah imobilisasi bakteri Pseudomonas aeruginosa pada matriks SA-PVA-Bentonit untuk mendekolorisasi MO. Hasil penelitian menunjukkan bahwa bakteri yang diimobilisasi dapat mendekolorisasi 50 mg/L MO sebesar 97,15% selama 10 hari, sedangkan bakteri sel bebas hanya mampu mendekolorisasi MO sebesar 43,17%. Jika dibandingkan terhadap sel bakteri mati yang diimobilisasi, matriks imobilisasi bakteri mati tetap mampu mendekolorisasi MO sebesar 57,58%. Hal tersebut menunjukkan bahwa bakteri P. aeruginosa yang terimobilisasi pada matriks SA-PVA-Bentonit memiliki kemampuan yang efektif dan efesien dalam mendekolorisasi MO (terutama sel hidup). Selain itu, pada penggunaan siklus berulang menunjukkan bahwa beads imobilisasi mampu digunakan hingga empat kali siklus, dengan persentase dekolorisasi MO yaitu 97,15; 93,10; 86,04; dan 41,23%. Kemudian pengujian karakterisasi juga dilakukan pada penelitian ini, seperti karakterisasi FTIR untuk mengidentifikasi gugus fungsi dan SEM untuk melihat kondisi topografi beads yang disintesis.
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Methyl orange (MO) dye is a synthetic dye that is widely used in the textile industry. MO waste can be toxic, mutagenic, and carcinogenic, if flowed out directly into the environment without any proper handling treatment. Bioremediation or biodecolorization using bacterial culture can be a solution to reduce MO waste. However, the use of bacteria using the free cell technique has several drawbacks, one of which is that it is susceptible to changing conditions or environmental stresses, such as limited amount of nutrients. The purpose of this study was to immobilize Pseudomonas aeruginosa bacteria on the SA-PVA-Bentonite matrix to decolorize MO. The results showed that immobilized bacteria could decolorize 50 mg/L MO by 97.15% for 10 days, while free cell bacteria were only able to decolorize MO by 43.17%. When compared to dead bacterial cells that were immobilized, the immobilized matrix of dead bacteria was still able to decolorize MO by 57.58%. These results show that P. aeruginosa bacteria immobilized on SA-PVA-Bentonite matrix have an effective and efficient ability to decolorize MO (especially living cells). In addition, the reuse cycles showed that the immobilized beads could be used up to four cycles, with the percentage of MO decolorization 97.15; 93.10; 86.04; and 41.23%. Then characterization tests were also carried out in this study, such as FTIR characterization to identify functional groups and SEM to see the topographic conditions of the synthesized beads.

Item Type:	Thesis (Other)
Additional Information:	RSKi 547.135 Mis b-1 2022
Uncontrolled Keywords:	Biodekolorisasi, Metil oranye, Pseudomonas aeruginosa, Imobilisasi, Bentonit. Biodecolorization, Methyl orange, Pseudomonas aeruginosa, Immobilization, Bentonite.
Subjects:	Q Science > QP Physiology > QP517.B5 Biodegradation.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	07 May 2026 04:11
Last Modified:	07 May 2026 04:11
URI:	http://repository.its.ac.id/id/eprint/133044

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