Pengembangan Sistem Kolorimetri Untuk Deteksi Multipleks Dan Multisampel Dengan μPAD Termodifikasi Grafena-Paladium.

Chano, Ignacius Gilbert (2022) Pengembangan Sistem Kolorimetri Untuk Deteksi Multipleks Dan Multisampel Dengan μPAD Termodifikasi Grafena-Paladium. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 02311840000051-Undergraduate_Thesis.pdf Restricted to Repository staff only Download (2MB)

Abstract

Sistem lab-on-chip dalam hal ini microfluidic paper based analytical devices (μPAD) memiliki beberapa kelebihan diantaranya penggunaan material kertas yang dapat digunakan dalam sekali pakai dan mudah didapat. Khususnya μPAD dengan deteksi kolorimetri memiliki tantangan pengembangan pada aspek keseragaman warna deteksi, kecepatan dan sensitivitas rentang deteksi linier. Untuk itu, pada penelitian ini dikembangkan sistem kolorimetri dengan rekayasa pencahayaan dan geometri μPAD untuk mendapatkan keseragaman warna deteksi. Selain itu, katalis nanokomposit Grafena-Pd disintesis secara hidrotermal dan ditambahkan dalam reagen pada zona deteksi μPAD untuk mendeteksi dan meningkatkan sensitivitas deteksi senyawa bio neurotransmitter dan glukosa. Persebaran pencahayaan pada zona deteksi μPAD yang optimal diperoleh dengan konfigurasi pencahayaan LED strip atas-bawah. Adanya penambahan nanokomposit Grafena-Pd pada permukaan kertas μPAD mengakibatkan permukaan kertas yang semakin kasar serta hidrofobik. Pengujian kolorimetri yang dilakukan dengan analit senyawa bio, diperoleh karakteristik hasil pengukuran time constant sebesar 12,071 detik, rentang deteksi glutamat pada konsentrasi 0,003 – 12 mM dengan sensitivitas 33,800 mM-1 dan linieritas R2 0,7480 pada ruang warna B (biru), serta rentang deteksi glukosa pada konsentrasi 50 – 300 mg/dL dengan sensitivitas -0,399 dL/mg dan linieritas R2 0,6900 pada ruang warna B.
===================================================================================================================================
The lab-on-chip system, i.e., microfluidic paper-based analysis (μPAD), exhibits several advantages, including the use of disposable paper materials which is easy to obtain. Particularly, μPAD with colorimetric detection has faced various challenges with regard to color uniformity, time response of detection, sensitivity and linear detection range. In this study, a colorimetric system with optimized lighting condition as well as μPAD geometry was developed for multiplex and multisample detection with higher color uniformity. In addition, the Graphene- Pd nanocomposite catalyst was hydrothermally synthesized and added in the reagent at the μPAD detection zone to detect and enhance the sensitivity detection of bio-neurotransmitters and glucose. The optimal luminance distribution in the μPAD detection zone is obtained with a top-down LED strip light configuration. The addition of Graphene-Pd nanocomposite on the surface of μPAD paper resulted in a rougher and a more hydrophobic surface. Colorimetry experiments with bio-analyte compounds indicate characteristic time constants of sensor’s response 12.071 seconds, detection range of glutamate’s concentration 0.003 – 12 mM with sensitivity 33.800 mM-1 and linearity from R2 value 0.776 on color space B (blue), also detection range of glucose’s concentration 50 – 300 mg/dL with sensitivity -0.399 dL/mg and linearity from R2 value 0.6900 on color space B (blue).

Item Type:	Thesis (Other)
Additional Information:	RSF 621.381 536 Cha p-1 2022
Uncontrolled Keywords:	μPAD, Multipleks, Multisampel, Sistem Kolorimetri. μPAD, Multiplex, Multisample, Colorimetric System.
Subjects:	T Technology > TA Engineering (General). Civil engineering (General) > TA1573 Detectors. Sensors
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	11 May 2026 06:06
Last Modified:	11 May 2026 06:06
URI:	http://repository.its.ac.id/id/eprint/133119

Actions (login required)

View Item