Aplikasi Cellobiose dehydrogenase (CDH) Sebagai Agen Biopestisida dalam Mengurangi Pertumbuhan Ralstonia solanacearum

Aisyah, Amilatus (2026) Aplikasi Cellobiose dehydrogenase (CDH) Sebagai Agen Biopestisida dalam Mengurangi Pertumbuhan Ralstonia solanacearum. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Ralstonia solanacearum merupakan salah satu patogen tular tanah yang menyebabkan penyakit layu batang terutama pada tanaman keluarga Solanaceae seperti cabai, tomat, kentang, dan terong. Penanganan penyakit layu batang umumnya dilakukan dengan menggunakan pestisida kimia yang dapat mencemari lingkungan serta menyebabkan resistensi pada bakteri patogen. Cellobiose dehydrogenase (CDH) dikenal sebagai enzim yang memiliki sifat antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi CDH sebagai agen biopestisida dalam mengurangi pertumbuhan bakteri R. solanacearum. Pada penelitian ini digunakan CDH dari jamur Trametes versicolor sebagai agen antibakteri dalam mengurangi pertumbuhan bakteri R. solanacearum penyebab penyakit layu batang. Metode penelitian meliputi produksi CDH dengan cara submerged fermentation menggunakan substrat kertas tisu, pemurnian dan karakterisasi CDH. Biopestisida dibuat dengan mereaksikan CDH aktivitas 2 U/mL dengan substrat diantaranya laktosa, maltosa, dan selulosa mikrokristalin (MCC). Aplikasi dan evaluasi biopestisida CDH dilakukan menggunakan metode inhibisi zona bening, MIC (Minimum Inhibitory Concentration) dan MBC (Minimum Bactericidal Concentration). Hasil penelitian menunjukkan jenis dan konsentrasi substrat CDH sebagai biopestisida berpengaruh terhadap pertumbuhan R. solanacearum. Jenis substrat laktosa paling berpengaruh diantara jenis substrat lain (maltosa dan MCC) serta bersifat bakteriostatik terhadap R. solanacearum pada konsentrasi 300 mM dengan diameter pembentukan zona bening 25,52 mm dan % inhibisi 97,5%. Jenis substrat maltosa berpengaruh sebagai bakteriostatik terhadap R. solanacearum pada konsentrasi 450 mM dengan diameter pembentukan zona bening 17,3 mm dan % inhibisi 98,85%. Sedangkan jenis substrat MCC tidak berpengaruh baik sebagai bakteriostatik maupun bakterisidal dengan diameter zona bening 2,53 mm dan % inhibisi yang tidak memenuhi nilai MIC.
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Ralstonia solanacearum is a soilborne pathogen that causes bacterial wilt disease, primarily affecting plants in the Solanaceae family such as chili peppers, tomatoes, potatoes, and eggplants. The control of bacterial wilt disease is generally carried out using chemical pesticides, which can pollute the environment and promote pathogenic bacterial resistance. cellobiose dehydrogenase (CDH) is recognized as an enzyme with antibacterial properties. This study aimed to investigate the effect of CDH application as a biopesticide agent in reducing the growth of R. solanacearum. In this research, CDH derived from the fungus Trametes versicolor was used as an antibacterial agent to inhibit the growth of R. solanacearum, the causative agent of bacterial wilt disease. The research methods included CDH production through submerged fermentation using tissue paper substrate, purification, and characterization of CDH. A biopesticide formulation was prepared by reacting CDH with an activity of 2 U/mL with various substrates including lactose, maltose, and microcrystalline cellulose (MCC). The application and evaluation of CDH biopesticide were conducted using the clear zone inhibition method, MIC (Minimum Inhibitory Concentration), and MBC (Minimum Bactericidal Concentration) assays. The results demonstrated that both the type and concentration of CDH substrate as a biopesticide significantly affected the growth of R. solanacearum. Lactose substrate showed the most significant effect among the tested substrates (maltose and MCC) and exhibited bacteriostatic properties against R. solanacearum at a concentration of 300 mM with a clear zone diameter of 25.52 mm and 97.5% inhibition. Maltose substrate showed bacteriostatic activity against R. solanacearum at a concentration of 450 mM with a clear zone diameter of 17.3 mm and 98.85% inhibition. Conversely, MCC substrate showed no significant bacteriostatic or bactericidal effect, with a clear zone diameter of 2.53 mm and inhibition percentage that did not meet the MIC threshold.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Agen antibakteri, Biopestisida, Cellobiose dehydrogenase, Ralstonia solanacearum, Trametes versicolor, Antibacterial agent, Biopesticide, Cellobiose dehydrogenase, Ralstonia solanacearum, Trametes versicolor.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QK Botany > QK710 Plant physiology Q Science > QK Botany > QK731 Growth (Plants) Q Science > QR Microbiology > QR74.8 Bacteria S Agriculture > S Agriculture (General) > S654.5 Biofertilizers
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Amilatus Aisyah
Date Deposited:	06 Jul 2026 00:48
Last Modified:	06 Jul 2026 00:49
URI:	http://repository.its.ac.id/id/eprint/134300

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