Hakim, Rafizaq (2026) Analisis Pengaruh Penambahan SrCl2 dan Na2HPO4 pada Hidrogel Injeksi berbasis HA-Alginate terhadap Karakterisasi Morfologi, Sifat Fisik, Mekanik, dan Biologi. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Injectable biomaterial hydrogel merupakan salah satu alternatif material yang menjanjikan untuk aplikasi rekayasa jaringan tulang karena dapat diaplikasikan secara minimal invasif dan mampu mengisi defek tulang dengan bentuk yang kompleks. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan strontium klorida (SrCl2) terhadap gelation time, sifat mekanik, morfologi, dan biokompatibilitas injectable biomaterial hidrogel berbasis nano-hydroxyapatite (HA), dicalcium phosphate dihydrate (DCPD), sodium alginate (Alg), dan glucono delta-lactone (GDL) dengan variasi konsentrasi SrCl2 dan Na2HPO4, kemudian dikarakterisasi melalui pengujian gelation time, viskositas dan injectability, analisis gugus fungsi menggunakan Fourier Transform Infrared (FTIR), identifikasi fase kristalin menggunakan X-ray Diffraction (XRD), pengamatan morfologi dan distribusi unsur menggunakan Scanning Electron Microscopy–Energy Dispersive X-ray (SEM–EDX), uji swelling, uji degradasi hingga hari ke-14, uji mekanik tekan pada beberapa waktu perawatan, uji aktivitas antibakteri, serta uji biokompatibilitas menggunakan metode MTT assay. Hasil penelitian menunjukkan bahwa penambahan SrCl2 mempercepat gelation time dari 14 ± 0.47 menit menjadi 6 ± 1.24 menit serta meningkatkan kekuatan tekan hingga 13.45 ± 0.23 MPa. Analisis SEM–EDX menunjukkan peningkatan ukuran pori dari 252.57 μm menjadi 531.26 μm serta distribusi unsur Sr yang relatif homogen dalam scaffold. Hasil FTIR dan XRD mengonfirmasi bahwa penambahan SrCl2 tidak membentuk gugus fungsi atau fase kristalin baru, namun memodifikasi interaksi ionik dan struktur scaffold yang telah ada. Uji degradasi menunjukkan peningkatan degradasi seiring waktu perendaman, dengan nilai tertinggi sebesar 40.37 ± 0.15 wt% pada hari ke-14, sementara uji MTT menunjukkan bahwa seluruh sampel bersifat tidak toksik dengan rentang viabilitas sel 82.56 ± 0.32% hingga 109.76 ± 0%. Berdasarkan hasil tersebut, dapat disimpulkan bahwa penambahan SrCl₂ mampu meningkatkan performa hidrogel injeksi HA/DCPD/Alg/GDL dengan tetap mempertahankan biokompatibilitas yang baik, sehingga berpotensi digunakan sebagai scaffold tulang injektabel.
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Injectable biomaterial hydrogels are promising materials for bone tissue engineering due to their minimally invasive application and ability to fill irregular bone defects. This study aims to evaluate the effect of strontium chloride (SrCl₂) addition on the gelation time, mechanical properties, morphology, and biocompatibility of injectable biomaterial hydrogels based on nano-hydroxyapatite (HA), dicalcium phosphate dihydrate (DCPD), sodium alginate (Alg), and glucono delta-lactone (GDL). The hydrogels were synthesized with varying concentrations of SrCl₂ and Na₂HPO₄, followed by characterization through gelation time, viscosity and injectability tests, Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy–Energy Dispersive X-ray (SEM–EDX) analysis, swelling and degradation tests up to 14 days, compressive strength testing at different curing times, antibacterial activity assessment, and biocompatibility evaluation using the MTT assay. The results demonstrated that the addition of SrCl₂ significantly reduced the gelation time from 14 ± 0.47 minutes to 6 ± 1.24 minutes and increased the compressive strength up to 13.45 ± 0.23 MPa. SEM–EDX analysis revealed an increase in average pore size from 252.57 μm to 531.26 μm with a relatively homogeneous distribution of Sr within the scaffold. FTIR and XRD results confirmed that the incorporation of SrCl₂ did not introduce new functional groups or crystalline phases but modified the ionic interactions and structural characteristics of the scaffold. Degradation tests showed a gradual increase in weight loss over time, reaching a maximum of 40.37 ± 0.15 wt% at day 14, while MTT assay results indicated that all samples were non-toxic with cell viability range between 82.56 ± 0.32% until 109.76 ± 0% in conclusion, the incorporation of SrCl₂ effectively enhances the functional performance of injectable hydrogels HA/DCPD/Alg/GDL while maintaining good biocompatibility, highlighting their potential as injectable bone scaffolds.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Hidrogel injeksi, scaffold tulang, biomaterial, strontium klorida, injectable hydrogel, bone scaffold, biomaterial, strontium chloride |
| Subjects: | R Medicine > R Medicine (General) > R857.M3 Biomedical materials. Biomedical materials--Testing. R Medicine > RD Surgery > RD755.6 Bone substitutes. T Technology > T Technology (General) |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Rafizaq Hakim |
| Date Deposited: | 13 Jul 2026 01:30 |
| Last Modified: | 13 Jul 2026 01:30 |
| URI: | http://repository.its.ac.id/id/eprint/134499 |
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