Profil Metabolit dan Isolasi Asam Sinamat dari Resin Styrax spp. serta Sintesis Senyawa Turunannya

Mustamu, Beatrice Christina Theodora (2026) Profil Metabolit dan Isolasi Asam Sinamat dari Resin Styrax spp. serta Sintesis Senyawa Turunannya. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Indonesia merupakan salah satu produsen resin kemenyan terbesar di dunia. Namun, ekspor kemenyan saat ini masih dalam bentuk bahan mentah sehingga Indonesia kehilangan potensi nilai tambah yang signifikan dari kekayaan hayati lokalnya. Resin kemenyan ini merupakan produk komoditas utama yang dihasilkan oleh tumbuhan Styrax spp. Untuk memetakan variasi keunggulan profil kimia Styrax spp. dengan komprehensif, penelitian ini memanfaatkan instrumen GC–MS dan LC–MS. Identifikasi profil metabolit sekunder dilakukan terhadap empat belas sampel resin untuk mengevaluasi pengaruh lokasi geografis, topografi lahan, varietas, dan periode panen terhadap komposisi senyawa mayor. Penelitian dilanjutkan dengan mengeksplorasi metode isolasi asam trans-sinamat (2b) dari matriks ekstrak resin, serta menyintesis senyawa turunannya berupa benzil sinamat (1) melalui metode esterifikasi substitusi nukleofilik bimolekuler. Hasil analisis statistik menunjukkan bahwa topografi lahan dan varietas memberikan pengaruh signifikan terhadap profil metabolit, sedangkan lokasi geografis dan periode panen tidak menunjukkan pengaruh yang signifikan. Isolasi asam trans-sinamat (2b) dengan metode ekstraksi cair-cair terbukti paling efektif dengan rendemen asam trans-sinamat (2b) murni sebesar 6,82%, sedangkan metode pelarutan selektif dinilai kurang selektif dalam memisahkan senyawa target. Proses esterifikasi berhasil mengonversi isolat asam trans-sinamat (2b) menjadi benzil sinamat (1) dengan rendemen sebesar 83,84% dalam waktu 16 jam 40 menit. Kemurnian asam trans-sinamat (2b) hasil isolasi dan benzil sinamat (1) hasil sintesis dikonfirmasi melalui analisis kromatografi lapis tipis dan 1H-Nuclear Magnetic Resonance, yang menunjukkan bahwa senyawa target berhasil diperoleh dengan kemurnian tinggi dan struktur yang sesuai.
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Indonesia is one of the world’s largest producers of benzoin resin. However, current Styrax export patterns remain heavily dominated by raw materials, leading to a significant loss of potential added value from Indonesia’s local biodiversity. To comprehensively map the variations in the chemical profiles of Styrax spp., this study utilized GC–MS and LC–MS instrumentation. Identification towards fourteen Styrax spp. resin's secondary metabolite profile is done to evaluate the influence of geographical location, land topography, variety, and harvest period towards each sample’s major component. The study proceeded by exploring various isolation methods for trans-cinnamic acid (2b) from the resin extract matrix, as well as synthesizing its derivative, benzyl cinnamate (1), via bimolecular nucleophilic substitution. Statistical analysis revealed that land topography and variety had a significant influence on the metabolite profiles, whereas geographical location and harvest period showed no significant effect. The isolation of trans-cinnamic acid (2b) using the liquid-liquid extraction method proved to be the most effective, with a yield of 6.82%, whereas the selective dissolution method was deemed not selective enough for target compound isolation. Furthermore, the esterification process successfully converted trans-cinnamic acid (2b) isolate into benzyl cinnamate (1) with a yield of 83.84% within a reaction time of 16 hours and 40 minutes. The purity of both the isolated trans-cinnamic acid (2b) and the synthesized benzyl cinnamate (1) was confirmed via thin-layer chromatography and 1H-Nuclear Magnetic Resonance, indicating that the target compounds were successfully obtained with high purity and confirmed structural integrity.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Styrax spp., Asam trans-sinamat, Profil metabolit, Ekstraksi cair-cair, Substitusi nukleofilik bimolekuler
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD305.A2 O73 Esterification Q Science > QD Chemistry > QD63.S4 Separation (Technology)
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Beatrice Christina Theodora Mustamu
Date Deposited:	15 Jul 2026 04:57
Last Modified:	15 Jul 2026 04:57
URI:	http://repository.its.ac.id/id/eprint/134899

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