Kamila, Farinda Atfa (2026) Green Synthesis dan Karakterisasi AgNP Terkonjugasi Turmeron Bioreduktor Daun Dewa (Gynura pseudochina L. (DC)) Teraplikasi Antibakteri Salmonella typhi dengan Aktivasi Fotodinamik dan Sonodinamik. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5001221157-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5001221157-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (6MB) | Request a copy |
Abstract
Infeksi bakteri yang disertai meningkatnya resistensi terhadap antibiotik konvensional mendorong pengembangan agen antibakteri alternatif berbasis nanoteknologi. Penelitian ini bertujuan mensintesis dan mengkarakterisasi AgNP menggunakan metode green synthesis berbasis ekstrak daun dewa (Gynura pseudochina), mengkonjugasikannya dengan turmeron (T-AgNP), serta mengevaluasi aktivitas antibakterinya terhadap Salmonella typhi melalui aktivasi fotodinamik dan sonodinamik. Sintesis AgNP dilakukan menggunakan ekstrak daun dewa sebagai bioreduktor dan penstabil, kemudian dikonjugasikan dengan turmeron. Karakterisasi meliputi UV-Vis, XRD, FESEM-EDX, FTIR, dan PSA. Aktivitas antibakteri diuji menggunakan metode difusi agar dengan perlakuan tanpa aktivasi, aktivasi fotodinamik menggunakan LED biru (460–475 nm), aktivasi sonodinamik menggunakan ultrasound terapeutik frekuensi 1 MHz, serta kombinasi keduanya. Hasil karakterisasi menunjukkan terbentuknya AgNP dengan puncak surface plasmon resonance (SPR) pada 460 nm, struktur kristal face-centered cubic (FCC), morfologi spherical–quasi spherical, serta perubahan gugus fungsi yang mengindikasikan keberhasilan konjugasi turmeron. Uji antibakteri menunjukkan AgNP memiliki aktivitas lebih tinggi dibandingkan T-AgNP tanpa aktivasi. Aktivasi fotodinamik dan sonodinamik meningkatkan diameter zona hambat, dengan peningkatan terbesar diperoleh pada kombinasi aktivasi sonodinamik dan fotodinamik yang menghasilkan zona hambat hingga 7 mm. Hasil penelitian menunjukkan bahwa aktivasi mampu meningkatkan efektivitas antibakteri AgNP hasil green synthesis.
================================================================================================================================
Bacterial infections accompanied by increasing resistance to conventional antibiotics have encouraged the development of alternative antibacterial agents based on nanotechnology. This study aimed to synthesize and characterize AgNP using a green synthesis approach with Gynura pseudochina leaf extract, conjugate them with turmerone (T-AgNP), and evaluate their antibacterial activity against Salmonella typhi under photodynamic and sonodynamic activation. AgNP were synthesized using the leaf extract as both reducing and stabilizing agents, followed by turmerone conjugation. The synthesized materials were characterized using UV–Vis spectroscopy, XRD, FESEM-EDX, FTIR, and PSA. Antibacterial activity was evaluated using the agar diffusion method under four conditions: without activation, photodynamic activation using a blue LED (460–475 nm), sonodynamic activation using a 1 MHz therapeutic ultrasound, and dual activation. Characterization results confirmed the successful formation of AgNP with a surface plasmon resonance (SPR) peak at 460 nm, a face-centered cubic (FCC) crystal structure, spherical to quasi-spherical morphology, and functional group shifts indicating successful turmerone conjugation. Antibacterial testing demonstrated that AgNP exhibited higher antibacterial activity than T-AgNP without activation. Both photodynamic and sonodynamic treatments enhanced the inhibition zone, with the highest antibacterial activity achieved under combined sonodynamic and photodynamic activation, producing an inhibition zone of up to 7 mm. These findings indicate that physical activation effectively enhances the antibacterial performance of green-synthesized AgNP.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Nanopartikel Perak, Sintesis Hijau, Daun Dewa, Turmeron, Fotodinamik, Ultrasound, Silver Nanoparticles, Green Synthesis, Gynura Pseudochina, Turmeron, Photodinamic, Ultrasound. |
| Subjects: | Q Science > QC Physics > QC451 Spectroscopy Q Science > QC Physics > QC457 Infrared technology. Q Science > QD Chemistry > QD716 Photocatalysis. |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45201-(S1) Undergraduate Thesis |
| Depositing User: | Farinda Atfa Kamila |
| Date Deposited: | 24 Jul 2026 07:48 |
| Last Modified: | 24 Jul 2026 07:48 |
| URI: | http://repository.its.ac.id/id/eprint/136255 |
Actions (login required)
 |
View Item |